Differential modulation of S-nitrosoglutathione reductase and reactive nitrogen species in wild and cultivated tomato genotypes during development and powdery mildew infection

Jahnová, Jana; Činčalová, Lucie; Sedlářová, Michaela; Jedelská, Tereza; Sekaninová, Jana; Mieslerová, B.; Luhová, Lenka; Barroso, Juan B.; Petřivalský, Marek

doi:10.1016/j.plaphy.2020.06.039

Cited by 7 publications

(5 citation statements)

References 85 publications

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“…Means significantly different from control are denoted by asterisks (ANOVA, *P < 0.05, ***P < 0.001) increased expression of tomato GSNOR enhanced pathogen protection 17 . However, our recent study showed that in Solanum spp.-Pseudoidium neolycopersici pathosystem reduced GSNOR activity and GSNO accumulation was related to a higher degree of tomato resistance to powdery mildew 24 . The observed discrepancies in modulations of plant GSNOR in response to pathogens might be related to diverse lifestyle and infection strategies among bacterial, fungal and oomycete phytopathogens.…”

Section: Discussionmentioning

confidence: 77%

“…Previously, we have uncovered specific roles of NO in tomato powdery mildew pathogenesis in studies on cultivated and wild tomato genotypes differing in their resistance 22,24,27 . Protein S-nitrosation (referred also to as protein S-nitrosylation) has emerged as a key NOdependent signalling pathway in plant responses to pathogen infection 31,32 .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, GSNOR was found down-regulated both on a local and systemic level in three tomatoe (Solanum spp.) genotypes infected with a biotrophic pathogen P. neolycopersici 24 .…”

Section: Introductionmentioning

confidence: 99%

“…Based on previous studies concerning NO roles in tomato powdery mildew pathogenesis 22,24,27 and detailed characterisation of tomato GSNOR in vitro 28 , we focused on the pivotal role of tomato GSNOR in interactions with hemibiotrophic oomycetes. Two tomato genotypes cultivated Solanum lycopersicum cv.…”

Section: Introductionmentioning

confidence: 99%

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Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

et al. 2021

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Regulation of protein function by reversible S-nitrosation, a post-translational modification based on the attachment of nitroso group to cysteine thiols, has emerged among key mechanisms of NO signalling in plant development and stress responses. S-nitrosoglutathione is regarded as the most abundant low-molecular-weight S-nitrosothiol in plants, where its intracellular concentrations are modulated by S-nitrosoglutathione reductase. We analysed modulations of S-nitrosothiols and protein S-nitrosation mediated by S-nitrosoglutathione reductase in cultivated Solanum lycopersicum (susceptible) and wild Solanum habrochaites (resistant genotype) up to 96 h post inoculation (hpi) by two hemibiotrophic oomycetes, Phytophthora infestans and Phytophthora parasitica. S-nitrosoglutathione reductase activity and protein level were decreased by P. infestans and P. parasitica infection in both genotypes, whereas protein S-nitrosothiols were increased by P. infestans infection, particularly at 72 hpi related to pathogen biotrophy–necrotrophy transition. Increased levels of S-nitrosothiols localised in both proximal and distal parts to the infection site, which suggests together with their localisation to vascular bundles a signalling role in systemic responses. S-nitrosation targets in plants infected with P. infestans identified by a proteomic analysis include namely antioxidant and defence proteins, together with important proteins of metabolic, regulatory and structural functions. Ascorbate peroxidase S-nitrosation was observed in both genotypes in parallel to increased enzyme activity and protein level during P. infestans pathogenesis, namely in the susceptible genotype. These results show important regulatory functions of protein S-nitrosation in concerting molecular mechanisms of plant resistance to hemibiotrophic pathogens.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

“…Recently, GSNOR was found down-regulated both on a local and systemic level in three tomatoe (Solanum spp.) genotypes infected with a biotrophic pathogen P. neolycopersici 24 .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

et al. 2021

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“…In plants, S-Nitrosylation is either catalyzed by thioredoxin or the GSNOR system, both of which strengthen the plant's immune response (Martínez-Medina et al, 2019a). Studies have also confirmed that plants displaying higher GSNOR activity are more resistant to powdery or downy mildew pathogens (Jahnova et al, 2020). In addition, treating potato plants with the inducers of SAR showed a steep increase in GSNOR activity and increased immunity against hemibiotrophic oomycete Phytophthora infestans (Jedelska et al, 2021).…”

Section: Sa and No: Interplay Between Biotic And Abiotic Stress Tolerance Biotic Stressmentioning

confidence: 99%

Salicylic Acid and Nitric Oxide: Insight Into the Transcriptional Regulation of Their Metabolism and Regulatory Functions in Plants

et al. 2021

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Salicylic acid (SA) and nitric oxide (NO) are key signaling molecules required to activate the plant's innate immunity against abiotic stresses and biotrophic attackers. Stress-induced signaling and accumulation of SA and NO triggers extensive transcriptional reprogramming of defense-related genes, induced biosynthesis of secondary metabolites and anti-microbial compounds, thereby protecting/steering plant growth and immunity. Transcriptional regulation of SA and NO signaling are crucial for fine-tuning important cellular and metabolic functions, thus making plant defense impervious against many pathogens. The development of an impenetrable immune response is often associated with an unavoidable trade-off in the form of active suppression of plant growth and reproduction. Therefore, we highlighted recent advancements and research to unravel transcriptional regulation of SA and NO signaling essential for fulfilling their role as defense signaling molecules. We also emphasized comprehensive knowledge related to transcriptional reprogramming of SA and NO signaling important in strengthening plant growth-immunity trade-off. We also highlighted the progress on SA and NO signaling playing an indispensable role in stimulating plant-microbe interaction to modulate crucial plant functions.

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The role of nitric oxide in systemic responses of plants

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Poór

2023

Nitric Oxide in Developing Plant Stress Resilience

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Differential modulation of S-nitrosoglutathione reductase and reactive nitrogen species in wild and cultivated tomato genotypes during development and powdery mildew infection

Cited by 7 publications

References 85 publications

Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp.

Salicylic Acid and Nitric Oxide: Insight Into the Transcriptional Regulation of Their Metabolism and Regulatory Functions in Plants

The role of nitric oxide in systemic responses of plants

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