Differential Modulation of Integrin Receptors and Extracellular Matrix Laminin by Transforming Growth Factor-β1 in Rat Alveolar Epithelial Cells

Kumar, Niranjan M.; Sigurdson, S.Lynn; Sheppard, Dean; Lwebuga‐Mukasa, Jamson S.

doi:10.1006/excr.1995.1389

Cited by 67 publications

(34 citation statements)

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“…To validate the dot blot immunoassay, HIEC were treated with TGF-␤ (1-10 ng/ml) under serum-free conditions for 72 h. It is known that TGF-␤1 promotes the secretion of extracellular matrix proteins such as laminin in rat and human cell lines (27,30). In our hands, TGF-␤ increases the expression of the two laminins produced by epithelial cells in a dose-dependent manner (Fig.…”

Section: Laminin Expression By Intestinal Epithelial Cells In Vitromentioning

confidence: 63%

Proinflammatory cytokines TNF-α and IFN-γ alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells

Francoeur¹,

Escaffit²,

Vachon³

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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Laminins are basement membrane molecules that mediate cell functions such as adhesion, proliferation, migration, and differentiation. In the normal small intestine, laminin-5 and -10 are mainly expressed at the base of villus cells. However, in Crohn's disease (CD), a major redistribution of these laminins to the crypt region of the inflamed ileal mucosa has been observed, suggesting a possible relationship between laminin expression and cytokine and/or growth factor production, which is also altered in CD. The aim of this study was to test the hypothesis that proinflammatory cytokines can modulate laminin expression by intestinal epithelial cells. The effect of TNF-α, IFN-γ, IL-1β, IL-6, and transforming growth factor (TGF)-β was analyzed on the expression of laminins in the normal human intestinal epithelial crypt (HIEC) cell line. When treated with a single cytokine, HIEC cells secreted small amounts of laminin-5 and -10. Only TNF-α and TGF-β induced a slight increase in the secretion of these laminins. However, in combination, TNF-α and IFN-γ synergistically stimulated the secretion of both laminin-5 and -10 in HIEC cells. Transcript analyses suggested that the upregulation of the two laminins might depend on distinct mechanisms. Interestingly, the TNF-α and IFN-γ combination was also found to significantly promote apoptosis. However, the effect of cytokines on the secretion of laminins was maintained even after completely blocking apoptosis by inhibiting caspase activities. These results demonstrate that laminin production is specifically modulated by the proinflammatory cytokines TNF-α and IFN-γ in intestinal epithelial cells under an apoptosis-independent mechanism.

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Section: Laminin Expression By Intestinal Epithelial Cells In Vitromentioning

confidence: 63%

Proinflammatory cytokines TNF-α and IFN-γ alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells

Francoeur¹,

Escaffit²,

Vachon³

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…The specific action exerted by TGF-b1 on b 1 integrin expression has not been explored in detail, and seems to vary according to the cell types under study [48]. However, a down-regulation of the a 3 by TGF-b1 has been reported in glomeruli from nephrotic rats [23], and rat alveolar epithelial cells cultured in the presence of this factor [49]. Our finding of a decreased expression of the a 3 in podocytes of hyperglycaemic animals, might thus be considered as an early effect of the high glucose levels and/or overexpression of TGF-b1 [46].…”

Section: Discussionmentioning

confidence: 99%

Alterations in the expression of the a 3 b 1 integrin in certain membrane domains of the glomerular epithelial cells (podocytes) in diabetes mellitus

Regoli

Bendayan

1997

Diabetologia

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“…Several studies (62)(63)(64) have shown that the ␣3␤1 integrin is decreased in the podocytes of humans and rats with diabetes. Furthermore, high glucose media in cultured rat or human podocytes decreases the expression of ␣3␤1 integrin (62,64); this downregulation is perhaps mediated by increased levels of TGF-␤1 (65,66).…”

Section: Podocyte Apoptosis And/or Detachment In Humans and Animal Momentioning

confidence: 97%

From the Periphery of the Glomerular Capillary Wall Toward the Center of Disease

2005

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Differential Modulation of Integrin Receptors and Extracellular Matrix Laminin by Transforming Growth Factor-β1 in Rat Alveolar Epithelial Cells

Cited by 67 publications

References 0 publications

Proinflammatory cytokines TNF-α and IFN-γ alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells

Proinflammatory cytokines TNF-α and IFN-γ alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells

Alterations in the expression of the a 3 b 1 integrin in certain membrane domains of the glomerular epithelial cells (podocytes) in diabetes mellitus

From the Periphery of the Glomerular Capillary Wall Toward the Center of Disease

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