Differential Modulation of Digestive Enzymes and Energy Reserves at Different Times after Feeding in Juveniles of the Marine Estuarine-Dependent Flatfish Paralichthys Orbignyanus (Valenciennes, 1839)

Albanesi, Camila P.; Méndez, Eugenia; Michiels, María Soledad; Radonic, Mariela; López, Andrea; Mañanes, Alejandra López

doi:10.2139/ssrn.4334343

Cited by 1 publication

(19 citation statements)

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“…Juveniles (mean length: 8.70 ± 0.50 cm, mean body weight: 9.37 ± 0.18 g) (INIDEP, Mar del Plata, Argentina) were maintained for 20 days, in six fiberglass circular tanks (100 L, 28 psu, pH 8.1, 22 ± 2 C, 12 h light/12 h dark) (Albanesi, Radonic, et al, 2021). Flounders were fed ad libitum 6 days a week with a balanced diet (Núcleo Alimentos MDQ SRL 41.8% carbohydrates, 46% proteins, 2.8% lipids).…”

Section: Fish Stock Holding Conditions and Husbandry Routinementioning

confidence: 99%

“…The α-amylase activity was determined colorimetrically by measuring the amount of maltose released (Biesiot & Capuzzo, 1990) with modifications (Albanesi, Radonic, et al, 2021). The reaction started with the addition of the corresponding sample to the reaction mixture containing the substrate starch (15 mg mL À1 ) in 50 mM phosphate buffer (pH 7.4) at 30 C. After 15 min of incubation, the reaction was stopped by adding 300 μL of the dinitrosalicylic acid reagent (DNS) (Miller, 1959), and the resultant mixture was maintained at 100 C for 10 min.…”

Section: Biochemical Assaysmentioning

confidence: 99%

“…Maltase and sucrase activities were assayed using a colorimetric method to determine the amount of glucose released from maltose and sucrose, respectively (Albanesi, Radonic, et al, 2021). The reaction was initiated by adding the sample to the reaction mixture containing the corresponding substrate (42 mM) in 0.1 M malate buffer (pH 6.4), and the mixture was incubated at 30 C for 10 min.…”

Section: Biochemical Assaysmentioning

confidence: 99%

“…Trypsin activity was determined using N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) (1.23 mM) as substrate (Albanesi, Radonic, et al, 2021). The reaction was initiated by adding BAPNA (final concentration, 1.23 mM) to a reaction mixture containing the sample in 50 mM Tris -HCl buffer (pH 9.0), 400 mM Cl 2 Ca at 45 C. After 15 min, the assay was stopped by adding 250 μL of 0.1 M KOH, and absorbance was determined at 405 nm.…”

Section: Biochemical Assaysmentioning

confidence: 99%

“…The determination of the activity of digestive enzymes at distinct times after feeding permits us to establish the postprandial temporal dynamics of central components of the metabolism of carbohydrates, proteins, and lipids and also to evaluate the potential effects of variable periods of food restriction on digestive parameters (Caruso et al, 2014;Pujante et al, 2018). The concomitant determination of the concentration of energy reserves in storage sites and morphological traits permits a further integrative understanding of the possible adjustments in digestive/metabolic parameters and body conditions (Albanesi et al, 2022;Albanesi, Radonic, et al, 2021;Caruso et al, 2014;Pujante et al, 2018). Although food restriction can generally have negative effects on fish metabolism and physiology (Choi et al, 2012;Yang et al, 2019), in various species it is beneficial in response to multiple stresses (Ensminger et al, 2021;Lu et al, 2019).…”

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confidence: 99%

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Differential modulation of digestive enzymes and energy reserves at different times after feeding in juveniles of the marine estuarine‐dependent flatfish Paralichthys orbignyanus (Valenciennes, 1839)

Albanesi,

Méndez,

Michiels

et al. 2023

Journal of Fish Biology

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Integrative studies are lacking on the responses of digestive enzymes and energy reserves in conjunction with morphological traits at distinct postprandial times in marine estuarine‐dependent flatfishes of ecological and economic importance, such as Paralichthys orbignyanus. We determined total weight (TW), hepato‐somatic index (IH), activities of digestive enzymes in the intestine, and the concentration of energy reserves in the liver and the muscle at 0, 24, 72, and 360 h after feeding in juveniles of P. orbignyanus. Amylase activity decreased at 72 h (about 30%). Maltase, sucrose, and lipase activities reached peak at 24 h (67%, 600%, and 35%, respectively). Trypsin and aminopeptidase‐N activities at 24 and 72 h, respectively, were lower than those at t = 0 (53% and 30%). A peak increase in the concentration of glycogen and triglycerides in the liver (24 h) (86% and 89%, respectively) occurred. In muscle, glycogen and triglyceride concentrations were unchanged at 24 h and higher at 72 and 360 h (100% and 60%). No changes were found in TW, IH, free glucose in the liver and muscle, and protein in the liver. The protein concentration in the muscle sharply increased at 24 and 360 h after feeding (60%). The results indicate a distinct and specific response of central components of carbohydrate, lipid, and protein metabolism that could be adjustments at the biochemical level upon periods of irregular feeding and even of long‐term food deprivation inside coastal lagoons or estuaries. The distinct responses of digestive enzymes in the intestine and energy reserves in the liver and muscle suggest the differential modulation of tissue‐specific anabolic and catabolic pathways that would allow the maintenance of physical conditions.

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Section: Fish Stock Holding Conditions and Husbandry Routinementioning

confidence: 99%