Differential modification of the C. elegans proteome in response to acute and chronic gamma radiation: Link with reproduction decline

Dubois, Cécile; Pophillat, Matthieu; Audebert, Stéphane; Fourquet, Patrick; Lecomte, Conrad; Dubourg, Nicolas; Galas, Simon; Camoin, Luc; Frelon, Sandrine

doi:10.1016/j.scitotenv.2019.04.039

Cited by 29 publications

(27 citation statements)

References 45 publications

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“…Analysis of global protein expression in this study showed the modulation of proteins involved in regulating biological processes such as lipid transport, DNA replication, development of germ cells, apoptosis, transport of ions, and the development of cuticles. These results confirmed that chronic irradiation induced different molecular mechanisms than acute irradiation [71].…”

Section: Effect On Agingsupporting

confidence: 79%

“…This was investigated in a study by Kuzmic et al, where they show an acceleration of the aging process by exposing C. elegans to chronic irradiation. Irrespective of the dose rate and dose delivered, chronic exposure reduced the level of oxidative protein damage (carbonylation), which is a biomarker of the aging process [70]; Dubois et al also experimented with the response of C. elegans to acute and chronic exposure [71]. After chronic exposure, 168 proteins were significantly changed in the experimental group, while 369 proteins were significantly changed following acute radiation exposure.…”

Section: Effect On Agingmentioning

confidence: 99%

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Review of Biological Effects of Acute and Chronic Radiation Exposure on Caenorhabditis elegans

Dhakal

Yosofvand

Yavari

et al. 2021

Cells

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Knowledge regarding complex radiation responses in biological systems can be enhanced using genetically amenable model organisms. In this manuscript, we reviewed the use of the nematode, Caenorhabditis elegans (C. elegans), as a model organism to investigate radiation’s biological effects. Diverse types of experiments were conducted on C. elegans, using acute and chronic exposure to different ionizing radiation types, and to assess various biological responses. These responses differed based on the type and dose of radiation and the chemical substances in which the worms were grown or maintained. A few studies compared responses to various radiation types and doses as well as other environmental exposures. Therefore, this paper focused on the effect of irradiation on C. elegans, based on the intensity of the radiation dose and the length of exposure and ways to decrease the effects of ionizing radiation. Moreover, we discussed several studies showing that dietary components such as vitamin A, polyunsaturated fatty acids, and polyphenol-rich food source may promote the resistance of C. elegans to ionizing radiation and increase their life span after irradiation.

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Section: Effect On Agingsupporting

confidence: 79%

Section: Effect On Agingmentioning

confidence: 99%

Review of Biological Effects of Acute and Chronic Radiation Exposure on Caenorhabditis elegans

Dhakal

Yosofvand

Yavari

et al. 2021

Cells

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“…Mass spectrometry analysis were done as already described previously 66 . Briefly, peptides obtained after trypsinisation were diluted in solvent A (0.1% v/v formic acid in 2% v/v acetonitrile) and concentrated using a pre-column (C18 PepMap100, 2 cm × 100 µm I.D, 100 Å pore size, 5 µm particle size, Dionex).…”

Section: Methodsmentioning

confidence: 99%

“…Data processing was performed using MaxQuant v1.6.1.0, computing relative intensities based on label-free quantification (LFQ) with the MaxLFQ algorithm. Mass spectra and differential protein expression analysis were performed as described before using Perseus v1.6.1.3 66 . Briefly precursors ions identification was performed using the Danio rerio UniProt protein database (12 th of February 2019 with 58994 entries) supplemented with 245 proteins commonly found as contaminants and a false positive rate of 1% for protein identification.…”

Section: Methodsmentioning

confidence: 99%

A systems biology approach reveals neuronal and muscle developmental defects after chronic exposure to ionising radiation in zebrafish

Houdigui

Adam-Guillermin

Loro

et al. 2019

Sci Rep

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Contamination of the environment after the Chernobyl and Fukushima Daiichi nuclear power plant (NPP) disasters led to the exposure of a large number of humans and wild animals to radioactive substances. However, the sub-lethal consequences induced by these absorbed radiological doses remain understudied and the long-term biological impacts largely unknown. We assessed the biological effects of chronic exposure to ionizing radiation (IR) on embryonic development by exposing zebrafish embryo from fertilization and up to 120 hours post-fertilization (hpf) at dose rates of 0.5 mGy/h, 5 mGy/h and 50 mGy/h, thereby encompassing the field of low dose rates defined at 6 mGy/h. Chronic exposure to IR altered larval behaviour in a light-dark locomotor test and affected cardiac activity at a dose rate as low as 0.5 mGy/h. The multi-omics analysis of transcriptome, proteome and transcription factor binding sites in the promoters of the deregulated genes, collectively points towards perturbations of neurogenesis, muscle development, and retinoic acid (RA) signaling after chronic exposure to IR. Whole-mount RNA in situ hybridization confirmed the impaired expression of the transcription factors her4.4 in the central nervous system and myogenin in the developing muscles of exposed embryos. At the organ level, the assessment of muscle histology by transmission electron microscopy (TEM) demonstrated myofibers disruption and altered neuromuscular junctions in exposed larvae at 5 mGy/h and 50 mGy/h. The integration of these multi-level data demonstrates that chronic exposure to low dose rates of IR has an impact on neuronal and muscle progenitor cells, that could lead to motility defects in free swimming larvae at 120 hpf. The mechanistic understanding of these effects allows us to propose a model where deregulation of RA signaling by chronic exposure to IR has pleiotropic effects on neurogenesis and muscle development.

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“…The Chernobyl nuclear accident on April 26 th 1986 was responsible for a large-scale release of radionuclides into the surrounding ecosystem, resulting in long-term exposure of the local and wider environment. Exposure to radioactivity in controlled, high dose laboratory studies has been shown to have effects on soil invertebrate species including earthworms (Hertel-Aas et al, 2007;Rusin et al, 2019;Sowmithra et al, 2015), springtails (Nakamori et al, 2008) and nematodes (Dubois et al, 2019;Lecomte-Pradines et al, 2017). Further, research on the long-term effects of ionising radiation in the surrounds of the former power station, now known as the Chernobyl Exclusion Zone or CEZ, have provided information on the physiological and ecological responses of individuals, populations and ecosystems to chronic radiation exposure (see Beresford et al, 2016;Geras'kin et al, 2008;Hinton et al, 2007;Lourenco et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic, epigenetic and microbiome characterisation of an earthworm species (Octolasion lacteum) along a radiation exposure gradient at Chernobyl

Newbold

Robinson

Rasnaca

et al. 2019

Environmental Pollution

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The effects of exposure to different levels of ionising radiation were assessed on the genetic, epigenetic and microbiome characteristics of the "hologenome" of earthworms collected at sites within the Chernobyl exclusion zone (CEZ). The earthworms Aporrectodea caliginosa (Savigny, 1826) and Octolasion lacteum (Örley, 1881) were the two species that were most frequently found at visited sites, however, only O. lacteum was present at sufficient number across different exposure levels to enable comparative hologenome analysis. The identification of morphotype O. lacteum as a probable single clade was established using a combination of mitochondrial (cytochrome oxidase I) and nuclear genome (Amplified Fragment Length Polymorphism (AFLP) using MspI loci). No clear site associated differences in population genetic structure was found between populations using the AFLP marker loci. Further, no relationship between ionising radiation exposure levels and the percentage of methylated loci or pattern of distribution of DNA methylation marks was found. Microbiome structure was clearly site dependent, with gut microbiome community structure and diversity being systematically associated with calculated site-specific earthworm dose rates. There was, however, also co-correlation between earthworm dose rates and other soil properties, notably soil pH; a property known to affect soil bacterial community structure. Such co-correlation means that it is not possible to attribute microbiome changes unequivocally to radionuclide exposure. A better understanding of the relationship between radionuclide exposure soil properties and their interactions on bacterial microbiome community response is, therefore, needed to establish whether these the observed microbiome changes are attributed directly to radiation exposure, other soil properties or to an interaction between multiple variables at sites within the CEZ. Capsule: Selected earthworm hologenome traits (e.g. gut microbiome) are potentially influenced by radiation exposure, but also confounding soil property variables

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Differential modification of the C. elegans proteome in response to acute and chronic gamma radiation: Link with reproduction decline

Cited by 29 publications

References 45 publications

Review of Biological Effects of Acute and Chronic Radiation Exposure on Caenorhabditis elegans

Review of Biological Effects of Acute and Chronic Radiation Exposure on Caenorhabditis elegans

A systems biology approach reveals neuronal and muscle developmental defects after chronic exposure to ionising radiation in zebrafish

Genetic, epigenetic and microbiome characterisation of an earthworm species (Octolasion lacteum) along a radiation exposure gradient at Chernobyl

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