Differential miRNA Expression Profiles in Cumulus and Mural Granulosa Cells from Human Pre-ovulatory Follicles

Andrei, Daniela; Nagy, Roland; Montfoort, Aafke P.A. van; Tietge, Uwe J. F.; Terpstra, Martijn; Kok, Klaas; Berg, Anke van den; Hoek, Annemieke; Kluiver, Joost; Donker, Rogier B.

doi:10.2174/2211536607666180912152618

Cited by 26 publications

(30 citation statements)

References 18 publications

(22 reference statements)

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“…were differentially expressed between mural FGCs and CCs (43). Top 10 most abundant miRNAs in mural GCs and CCs were miR Previous studies also demonstrated miR-146a-5p promoting apoptosis of mural GCs by directly targeting IRAK1 and TRAF6 (48) and miR-503-5p inhibiting proliferation by targeting CCND2 (49).…”

Section: Mirnas Regulate Development Of Mural Gcs and Cumulus Cells (mentioning

confidence: 92%

“…A c c e p t e d A r t i c l ehsa-miR-125b-5p, hsa-miR-1275, hsa-miR-129-5p and hsa-miR-129-2-3p) in CC were upregulated(43).Overall together, studies on cell communication, extracellular matrix and signaling pathways have demonstrated the differential expressions of miRNAs have relevance with physiological functions of CCs and mural GCs(45).MiRNAs regulate development of ovarian folliclesBoth follicular development (FD) and oocyte maturation are completed in the ovaries of female mammals. A highly complicated, spontaneous death phenomenon that is called as atresia takes place during the FD and maturation in the mammals.…”

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confidence: 99%

“…However, it has been undetermined which miRNA cluster(s) are associated with the each stage of FD(62,64,65). Furthermore, actual roles of these miRNA clusters in the FD, atresia and ovulation remain unclear(8,43,69).Fifteen different miRNAs were found during the growth and selection of dominant follicles(70).Six miRNAs, including miR-17, miR-18a, miR-19a, miR-20a, miR19b and miR-92a, are encoded by a single miR-17-92 transcript (71), and are expressed and processed together as a cluster (64). The miR-17-92 cluster was differentially expressed in granulosa cells from subordinate and dominant follicles at day 19 of the estrous cycle (72).…”

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confidence: 99%

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MicroRNAs regulate granulosa cells apoptosis and follicular development — A review

Gong

Yang

Bai

et al. 2020

Asian-Australas J Anim Sci

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Objective: MicroRNAs (miRNAs) are the most abundant small RNAs. Approximately annotated miRNAs genes have been found to be differentially expressed in ovarian follicles during the follicular development. Many miRNAs exert their regulatory effects on the apoptosis of follicular granulosa cells (FGCs) and follicular development (FD). However, accurate roles and mechanism of miRNAs regulating apoptosis of FGCs remain undetermined. Methods: In this review, we summarized the regulatory role of each miRNA or miRNA cluster on FGCs apoptosis and FD on the bases of 41 academic articles retrieved from PubMed and web of science and other databases. Results: Total of 30 miRNAs and 4 miRNAs clusters in 41 articles were reviewed and summarized in the present article. 29 documents indicated explicitly that 24 miRNAs and miRNAs clusters in 29 articles promoted or induced FGCs apoptosis through their distinctive target genes. The remaining 10 miRNAs and miRNAs of 12 articles inhibited FGCs apoptosis. were reported in all articles. MiRNAs exerted modulation actions by at least 77 signal pathways during FGCs apoptosis and FD. Conclusion: We concluded that miRNAs or miRNAs clusters could modulate the apoptosis of GCs (including follicular GCs, mural GCs and cumulus cells) by targeting their specific genes. A great majority of miRNAs show promoting role on apoptosis of FGCs in mammals. But the accurate mechanism of miRNAs and miRNA clusters has not been well understood. It is extremely necessary to ascertain clearly the role and mechanism of each miRNA or miRNA cluster in the future. Understanding precise functions and mechanisms of miRNAs in FGCs apoptosis and FD will be beneficial for finding new diagnostic and treatment strategy or scheme for infertility and ovarian diseases in humans and animals.

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Section: Mirnas Regulate Development Of Mural Gcs and Cumulus Cells (mentioning

confidence: 92%

mentioning

confidence: 99%

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confidence: 99%

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MicroRNAs regulate granulosa cells apoptosis and follicular development — A review

Gong

Yang

Bai

et al. 2020

Asian-Australas J Anim Sci

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“…There are two specialised cell types of GCs, with distinct functions, called mural GCs and cumulus cells [18,[24][25][26]. During folliculogenesis, from the transition of the preantral and antral follicle stage, mural GCs and cumulus cells are made from a common progenitor.…”

Section: Characteristics Of Granulosa Cellsmentioning

confidence: 99%

“…Furthermore, exosomes carry proteins and MicroRNAs (miRNAs) [5]. With the technique of small RNA sequencing, mural GCs and cumulus cells have been shown to secrete over 50 miRNAs differentially [25]. In folliculogenesis, oogenesis, tumorigenesis and tumour suppression, miRNAs are tightly involved in the transforming growth factor (TGF)-β signalling pathway [35].…”

Section: Characteristics Of Exosomes and Cell Stressmentioning

confidence: 99%

Ovarian follicular cells - living in the shadow of stemness cellular competence

Moncrieff

Mozdziak

Ješeta

et al. 2019

Medical Journal of Cell Biology

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Granulosa cells (GCs) are a major component found in ovarian follicular fluid among oocytes, theca cells, and ovarian surface epithelial (OSE) cells. GCs are steroidogenic and have morphological functions that are important for the development of the follicular follicle. The follicle protects the developing female egg. GCs are also essential for the maturation of the female germ cell. Stem cell properties have been found in luteinised GCs and in vivo cultures have the potential to differentiate to become cells found outside of the ovary. Both three-dimensional (3D) culturing and mouse embryonic fibroblast (MEF) medium have been used to help improve the culturing lifespan of GCs so that their profound proliferation and differentiation capabilities can be studied. Small RNAs called MicroRNAs (miRNAs) are released from exosomes originating from GCs, and they are involved in transforming growth factor (TGF)-β signalling, follicle-stimulating hormone, hormone-related miRNAs, and apoptosis-related pathway. Finding the miRNAs involved in these pathways, and the mechanisms controlling GCs are important to treating conditions like polycystic ovary syndrome (PCOS), and creating new drug therapies. Besides GCs, ovarian stem cells (OSCs) were discovered in the OSE, and they are believed to be derived from very small embryonic stem cells (VSELs). Transplanting blood mononuclear cells, cell Hormone Therapy (cHT) with bone marrow stem cell supplement and increasing vitamin C levels are all therapies currently being researched into that involve GCs to combat the effects of aging and infertility.Running title: Stemness of ovarian follicular cells

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Role of Bioinformatics in MicroRNA Analysis

Mani

2021

Advances in Bioinformatics

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Differential miRNA Expression Profiles in Cumulus and Mural Granulosa Cells from Human Pre-ovulatory Follicles

Abstract: We generated miRNA expression profiles for MGCs and CCs and identified several differentially expressed miRNAs.

Cited by 26 publications

References 18 publications

MicroRNAs regulate granulosa cells apoptosis and follicular development — A review

MicroRNAs regulate granulosa cells apoptosis and follicular development — A review

Ovarian follicular cells - living in the shadow of stemness cellular competence

Role of Bioinformatics in MicroRNA Analysis

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