Differential membrane targeting of the SERCA and PMCA calcium pumps: experiments with recombinant chimeras

Guerini, Danilo; Guidi, Fabrizio; Carafoli, Ernesto

doi:10.1096/fj.01-0362com

Cited by 10 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Evidence of a Calcium-pumping ATPase Needed for Excystation-In most eukaryotic cells, Ca 2ϩ pump activity due to SERCA is specifically inhibited by the sesquiterpene lactone, TG (36). The addition of 5 M TG (Calbiochem) to Indo-1 loaded trophozoites, cysts, or excysting cells produced an immediate and sustained rise in Indo-1 fluorescence, corresponding to an increase in free intracellular Ca 2ϩ (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The rapid and robust increase in giardial [Ca 2ϩ ] i in response to TG suggests that this storage system may have a large capacity to store Ca 2ϩ and that the Ca 2ϩ cycles into and out of the storage compartment rapidly. Thus, kinetically, the TG-sensitive Ca 2ϩ storage compartment in Giardia resembles the SERCA pump-leak system found in many mammalian cells (18,36). The ability of G. lamblia to release Ca 2ϩ into the cytoplasm in response to TG was evident throughout the life cycle.…”

Section: And This Work)mentioning

confidence: 88%

See 1 more Smart Citation

Calcium Signaling in Excystation of the Early Diverging Eukaryote, Giardia lamblia

Reiner

Hetsko

Meszaros

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

Excystation of Giardia lamblia, which initiates infection, is a poorly understood but dramatic differentiation induced by physiological signals from the host. Our data implicate a central role for calcium homeostasis in excystation. Agents that alter cytosolic Ca 2؉ levels (1,2-bis(2-aminophenoxy)ethane-N,N,N ,N -tetraacetic acidtetra(acetyloxymethyl) ester, a Ca 2؉ channel blocker, Ca 2؉ ionophores, and thapsigargin) strongly inhibit excystation. Treatment of Giardia with thapsigargin raised intracellular Ca 2؉ levels, and peak Ca 2؉ responses increased with each stage of excystation, consistent with the kinetics of inhibition. Fluorescent thapsigargin localized to a likely Ca 2؉ storage compartment in cysts. The ability to sequester ions in membranebounded compartments is a hallmark of the eukaryotic cell. These studies support the existence of a giardial thapsigargin-sensitive Ca 2؉ storage compartment resembling the sarcoplasmic/endoplasmic reticulum calcium ATPase pump-leak system and suggest that it is important in regulation of differentiation and appeared early in the evolution of eukaryotic cells. Calmodulin antagonists also blocked excystation. The divergent giardial calmodulin localized to the eight flagellar basal bodies/centrosomes, like protein kinase A. Inhibitor kinetics suggest that protein kinase A signaling triggers excystation, whereas calcium signaling is mainly required later, for parasite activation and emergence.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: And This Work)mentioning

confidence: 88%

Calcium Signaling in Excystation of the Early Diverging Eukaryote, Giardia lamblia

Reiner

Hetsko

Meszaros

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…SERCA2 encodes a predicted protein of 996 amino acids that is 84% identical to human SERCA2. Moreover, zebrafish and human SERCA2 are 75% identical in the first 28 amino acids, a critical region for protein retention in the SR (25). Zebrafish SERCA2 is expressed robustly in the cardiac precursors, throughout the forming heart, and in skeletal muscle (Fig.…”

Section: Resultsmentioning

confidence: 99%

Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts

Ebert

Hume

Warren

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

126

View full text Add to dashboard Cite

Calcium entry into myocytes drives contraction of the embryonic heart. To prepare for the next contraction, myocytes must extrude calcium from intracellular space via the Na ؉ ͞Ca 2؉ exchanger (NCX1) or sequester it into the sarcoplasmic reticulum, via the sarcoplasmic reticulum Ca 2؉ -ATPase2 (SERCA2). In mammals, defective calcium extrusion correlates with increased intracellular calcium levels and may be relevant to heart failure and sarcoplasmic dysfunction in adults. We report here that mutation of the cardiac-specific NCX1 (NCX1h) gene causes embryonic lethal cardiac arrhythmia in zebrafish tremblor (tre) embryos. The tre ventricle is nearly silent, whereas the atrium manifests a variety of arrhythmias including fibrillation. Calcium extrusion defects in tre mutants correlate with severe disruptions in sarcomere assembly, whereas mutations in the L-type calcium channel that abort calcium entry do not produce this phenotype. Knockdown of SERCA2 activity by morpholino-mediated translational inhibition or pharmacological inhibition causes embryonic lethality due to defects in cardiac contractility and morphology but, in contrast to tre mutation, does not produce arrhythmia. Analysis of intracellular calcium levels indicates that homozygous tre embryos develop calcium overload, which may contribute to the degeneration of cardiac function in this mutant. Thus, the inhibition of NCX1h versus SERCA2 activity differentially affects the pathophysiology of rhythm in the developing heart and suggests that relative levels of NCX1 and SERCA2 function are essential for normal development.heart development ͉ arrhythmia ͉ sodium calcium exchanger ͉ Ca 2ϩ -ATPase

show abstract

“…This is compatible with the behavior of a transmembrane protein that is free to laterally diffuse in the lipid bilayer. In fact, electron microscopy studies had provided evidence that SERCA particles are widely distributed in the longitudinal SR (6), and no evidence that SERCA pumps are associated with other cellular structures has been reported (31). Thus, the SERCA immunofluorescence signal observed in bands at the Z disk, and to a lesser extent to the M band, rather than reflecting a specific retention mechanism, may result from the higher density of SR membranes in these regions (9,24).…”

Section: Organization and Mobility Properties Of Longitudinal Sr Protmentioning

confidence: 99%

Assembly and dynamics of proteins of the longitudinal and junctional sarcoplasmic reticulum in skeletal muscle cells

Cusimano

Pampinella

Giacomello

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The sarcoplasmic reticulum (SR) of skeletal muscle cells is a complex network of tubules and cisternae that share a common lumen delimited by a single continuous membrane. The SR contains longitudinal and junctional domains characterized by distinctive patterns of protein localization, but how SR proteins reach and/or are retained at these sites is not known. Here, we report that the organization of longitudinal SR proteins is a slow process characterized by temporally distinct patterns of protein localization. In contrast, junctional SR proteins rapidly and synchronously assembled into clusters which, however, merged into mature triadic junctions only after completion of longitudinal SR protein organization. Fluorescence recovery after photobleaching experiments indicated that SR organization was accompanied by significant changes in the dynamic properties of longitudinal and junctional proteins. The decrease in mobility that accompanied organization of the longitudinal SR proteins ank1.5-GFP and GFP-InsP3R1 was abrogated by deletion of specific binding sites for myofibrillar or cytoskeletal proteins, respectively. Assembly of junctional SR domains was accompanied by a strong decrease in mobility of junctional proteins that in triadin appeared to be mediated by its intraluminal region. Together, the data suggest that the organization of specific SR domains results from a process of membrane reorganization accompanied by the establishment of multiple protein-protein interactions with intrinsic and extrinsic cues.calcium store ͉ excitation-contraction coupling ͉ muscle differentiation ͉ protein dynamics

show abstract

Differential membrane targeting of the SERCA and PMCA calcium pumps: experiments with recombinant chimeras

Cited by 10 publications

References 37 publications

Calcium Signaling in Excystation of the Early Diverging Eukaryote, Giardia lamblia

Calcium Signaling in Excystation of the Early Diverging Eukaryote, Giardia lamblia

Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts

Assembly and dynamics of proteins of the longitudinal and junctional sarcoplasmic reticulum in skeletal muscle cells

Contact Info

Product

Resources

About