Differential lineage-specific regulation of murine CD45 transcription by Oct-1 and PU.1

Kwon, Un K.; Yen, Pei-Hua; Collins, Tara J.; Wells, Richard A.

doi:10.1016/j.bbrc.2006.03.119

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…within the CD10 promoter fragment we have used, and PU.1 has been shown to bind to some AAGGAA sequences (50,51). One of these AAGGAA sites (PU-4) is found within the first region of the CD10 promoter (nucleotides 1-470) and five of these sites are clustered in the second region of the promoter (nucleotides 449 -717) (supplemental Fig.…”

Section: Discussionmentioning

confidence: 99%

NF-κB Down-regulates Expression of the B-lymphoma Marker CD10 through a miR-155/PU.1 Pathway

Thompson¹,

Herscovitch²,

Zhao

et al. 2011

Journal of Biological Chemistry

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The NF-B signaling pathway is misregulated in a variety of human diseases including many chronic inflammatory diseases and cancers. As such, an understanding of the molecular details of NF-B-dependent gene networks has implications for improved disease diagnoses and therapies. CD10, also known as the common acute lymphocytic leukemia antigen (CALLA) or neutral endopeptidase, is a cellsurface zinc metalloendopeptidase (1, 2). The ability of CD10 to cleave signal peptides at the cell surface can affect cell proliferation, differentiation, and migration (2-4). Expression of CD10 can be used as a diagnostic marker for a variety of cancers (5-9). Relevant to this study, CD10 is highly expressed in the germinal center B-cell (GCB) 6 molecular subtype of diffuse large B-cell lymphoma (DLBCL), whereas CD10 expression is low in the activated B-cell (ABC) subtype of DLBCL (8, 9). ABC DLBCLs also have a high NF-B gene expression profile and a poorer clinical prognosis as compared with GCB DLBCLs (10). As such, reduced expression of CD10 and high NF-B activity are both correlated with a less favorable DLBCL patient outcome (10 -12).The correlation between high NF-B activity and reduced CD10 expression has been observed in several other settings as well. We previously showed that overexpression of an activated mutant of the NF-B family transcription factor REL in the GCB-like B-lymphoma cell line BJAB leads to reduced expression of CD10 (13). Infection of cells with Epstein-Barr virus (EBV) or human cytomegalovirus, both inducers of NF-B, causes reduced expression of CD10 (14, 15). Taken together, such results suggested to us that NF-B or a target of NF-B is involved in repressing CD10 gene/protein expression in certain B-cell lymphomas.Little is known about the control of CD10 transcription. Sequence analysis of the CD10 promoter/enhancer region revealed the presence of three consensus binding sites for transcription factor PU.1 (16). PU.1 is a member of the Ets family of transcription factors and is required for proper Bcell development and differentiation (17). Additionally, increased PU.1 expression has been correlated with the GCB subtype of DLBCL (8). Therefore, we were interested in investigating whether PU.1 contributes to the regulation of CD10 expression in B-cell lymphoma.In this report, we provide evidence for a functional link between activation of NF-B and reduced expression of CD10. We show that activation of NF-B in the GCB-like DLBCL cell line BJAB leads to reduced CD10 expression. Our data are consistent with a pathway in which NF-B-induced up-regulation of micro-RNA miR-155 leads to down-regulation of PU.1 protein levels, and consequently, reduced levels of * This work was supported, in whole or in part, by National Institutes of Health Grant CA047763 and American Recovery and Reinvestment Act of 2009 supplement Grant CA047763021S3 (to T. D. G.). □ S The on-line version of this article (available at http://www.jbc.org) contains supplemental Table S1 and Fig. S1

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Section: Discussionmentioning

confidence: 99%

NF-κB Down-regulates Expression of the B-lymphoma Marker CD10 through a miR-155/PU.1 Pathway

Thompson¹,

Herscovitch²,

Zhao

et al. 2011

Journal of Biological Chemistry

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“…Members of this family recognize and bind to the octameric sequence ATGCAAAT to activate transcription of various genes [ 126 ]. Oct-1 and PU.1 is involved in the control of the murine CD45 (Ptprc) promoter activity [ 127 ]. Characterization of the promoter region of the Fibroleukin (FGL2) gene revealed that the cis -elements, Oct-1, Ets1 and Sp1/Sp/3, co-operatively regulate its transcription [ 128 , 129 ].…”

Section: Co-operation Of the Ggaa Motifs With Other Transcription Facmentioning

confidence: 99%

The possible functions of duplicated ets (GGAA) motifs located near transcription start sites of various human genes

Uchiumi

Miyazaki

Tanuma

2011

Cell. Mol. Life Sci.

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Transcription is one of the most fundamental nuclear functions and is an enzyme complex-mediated reaction that converts DNA sequences into mRNA. Analyzing DNA sequences of 5′-flanking regions of several human genes that respond to 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in HL-60 cells, we have identified that the ets (GGAA) motifs are duplicated, overlapped, or clustered within a 500-bp distance from the most 5′-upstream region of the cDNA. Multiple protein factors including Ets family proteins are known to recognize and bind to the GGAA containing sequences. In addition, it has been reported that the ets motifs play important roles in regulation of various promoters. Here, we propose a molecular mechanism, defined by the presence of duplication and multiplication of the GGAA motifs, that is responsible for the initiation of transcription of several genes and for the recruitment of binding proteins to the transcription start site (TSS) of TATA-less promoters.

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“…Ο CD15 αποτελεί το αντιγόνο Lewis X, έναν υδατάνθρακα που απαντάται σε κακοήθη νεοπλασματικά κύτταρα214 . Ο CD33 χαρακτηρίζει πρόδρομα κύτταρα της μυελικής σειράς, ενώ η έκφραση των CD34 και CD45 προσδιορίζει τα πολυδύναμα βλαστικά αιμοποιητικά κύτταρα (Haematopoietic Stem Cells-HSC), από τα οποία προκύπτουν οι κυτταρικές σειρές του αίματος215,216 . Με βάση τον ανοσοφαινότυπο που προσδιορίστηκε με τη μέθοδο της κυτταρομετρίας ροής ταυτοποιήθηκε ο πληθυσμός των αρχέγονων μεσεγχυματικών κυττάρων ο οποίος και υποβλήθηκε κατόπιν στην επίδραση της 5-aza σύμφωνα με το σχεδιασμό του ερευνητικού πρωτοκόλλου.…”

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Μελέτη Της Μετατροπής Των Αρχέγονων Αιμοποιητικών Κυττάρων Σε Μυοκαρδιακά Κύτταρα Εντός Συστήματος Υγρής Καλλιέργειας Μακράς Διάρκειας

Αντωνίτσης¹

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Σκοπός της παρούσας μελέτης αποτελεί η διερεύνηση της ικανότητας των αρχέγονων μεσεγχυματικών κυττάρων του μυελού των οστών να μετατρέπονται σε μυοκαρδιακά κύτταρα του μυελού των οστών να μετατρέπονται σε μυοκαρδιακά κύτταρα σε σύστημα υγρής καλλιέργειας. Δείγματα μυελού ελήφθησαν από 30 ασθενείς οι οποίοι υποβλήθηκαν σε χειρουργική επέμβαση ανοικτής καρδιάς. Τα μεσεγχυματικά βλαστικά κύτταρα απομονώθηκαν και τοποθετήθηκαν σε καλλιέργεια. Μετά τη δεύτερη ανακαλλιέργεια έγινε επίδραση 10μ.Μ 5-αζακυτιδίνης. Ο ανοσοφαινότυπος προσδιορίστηκε με κυτταρομετρία ροής. Η μελέτη της μορφολογίας διενεργήθηκε με το ανάστροφο καθώς και με το ηλεκτρονικό μικροσκόπιο. Πραγματοποιήθηκε ανοσοϊστοχημικός έλεγχος της βιμεντίνης και της βαριάς αλύσου της μυοσίνης. Μελετήθηκε παράλληλα η έκφραση των γονιδίων της α-καρδιακής ακτίνης και της β-βαριάς αλύσου της μυοσίνης και της τροπονίνης-Τ. Στα κύτταρα που υποβλήθηκαν στην επίδραση ανιχνεύτηκαν πολυάριθμα ινίδια, τα οποία ήταν θετικά με ανοσοϊστοχημεία στη βαριά άλυσο μυοσίνης. Παράλληλα διαπιστώθηκε έκφραση των υπό μελέτη γονιδίων. Αντίθετα, σε κύτταρα πολύ δεν υποβλήθηκαν σε καλλιέργεια, δε διαπιστώθηκε έκφραση μυοκαρδιακών δεικτών.

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Differential lineage-specific regulation of murine CD45 transcription by Oct-1 and PU.1

Cited by 6 publications

References 31 publications

NF-κB Down-regulates Expression of the B-lymphoma Marker CD10 through a miR-155/PU.1 Pathway

NF-κB Down-regulates Expression of the B-lymphoma Marker CD10 through a miR-155/PU.1 Pathway

The possible functions of duplicated ets (GGAA) motifs located near transcription start sites of various human genes

Μελέτη Της Μετατροπής Των Αρχέγονων Αιμοποιητικών Κυττάρων Σε Μυοκαρδιακά Κύτταρα Εντός Συστήματος Υγρής Καλλιέργειας Μακράς Διάρκειας

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