Differential light-scattering of granal and agranal chloroplasts and their fragments

Faludi-Dániel, Ágnes; Bialek, G E; Horváth, Gábor; Sz-Rózsa, Z; Gregory, R. P. F.

doi:10.1042/bj1740647

Cited by 10 publications

(6 citation statements)

References 12 publications

(13 reference statements)

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“…In green sectors, the light absorption by chlorophyll reduced overall scattering although chloroplast grana caused light scattering around 682 nm (Fig. 6; Faludi‐Daniel et al . 1978).…”

Section: Discussionmentioning

confidence: 99%

“…As the chloroplasts move between the low-and high-light arrangements, the same surface of the chloroplast remains attached to the plasma membrane, thus resulting in the re-orientation of the chloroplast and the grana. Light is scattered by grana (Faludi-Daniel et al 1978) so chloroplast movements have the potential to alter light scattering in leaves. In the majority of species tested, light scattering decreased slightly in leaves following high-light treatment (Table 2).…”

Section: Discussionmentioning

confidence: 99%

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Changes in leaf optical properties associated with light‐dependent chloroplast movements

Davis

Caylor

Whippo

et al. 2011

Plant Cell & Environment

101

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We surveyed 24 plant species to examine how leaf anatomy influenced chloroplast movement and how the optical properties of leaves change with chloroplast position. All species examined exhibited light-dependent chloroplast movements but the associated changes in leaf absorptance varied considerably in magnitude. Chloroplast movementdependent changes in leaf absorptance were greatest in shade species, in which absorptance changes of >10% were observed between high-and low-light treatments. Using the Kubelka-Munk theory, we found that changes in the absorption (k) and chlorophyll a absorption efficiency (k*) associated with chloroplast movement correlated with cell diameter, such that the narrower, more columnar cells found in sun leaves restricted the ability of chloroplasts to move. The broader, more spherical cells of shade leaves allowed greater chloroplast rearrangements and in lowlight conditions allowed efficient light capture. Across the species tested, light-dependent chloroplast movements modulated leaf optical properties and light absorption efficiency by manipulating the package (sieve or flattening) effect but not the detour (path lengthening) effect.

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“…In green sectors, the light absorption by chlorophyll reduced overall scattering although chloroplast grana caused light scattering around 682 nm (Fig. 6; Faludi‐Daniel et al . 1978).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Changes in leaf optical properties associated with light‐dependent chloroplast movements

Davis

Caylor

Whippo

et al. 2011

Plant Cell & Environment

101

View full text Add to dashboard Cite

show abstract

“…An involvement of light scattering in this CD signal was first recognized by Philipson and Sauer (1973) and investigated in more detail by Gregory and Raps (1974) and Faludi-Daniel et al (1978). The data, however, do not allow firm statements to be made concerning the significance of the light-scattering component in defining the overall CD signal.…”

mentioning

confidence: 92%

Macroorganization of chlorophyll a/b light-harvesting complex in thylakoids and aggregates: information from circular differential scattering

Garab¹,

Faludi-Dániel²,

Sutherland³

et al. 1988

Biochemistry

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Circular dichroism (CD) and magnetic circular dichroism (MCD) spectra were recorded for spinach thylakoids and for isolated, aggregated chlorophyll a / b light-harvesting pigment-protein complex, in random and magnetically aligned states of orientation at room and low temperatures. The shape and magnitude of the C D signal of most bands strongly depended on the orientation of the thylakoid membranes or the aggregated pigment-protein complex. In both thylakoids and aggregated light-harvesting complexes, however, the MCD spectra of the two different orientations were almost identical. Random and magnetically aligned samples exhibited anomalous, large C D signals outside the bands of pigment absorbance. Lack of similarity between the corresponding M C D and C D spectra showed that the large C D signals are not produced as a distortion of CD of absorbance by light scattering. Instead, these anomalous spectral features are believed to originate in differential selective scattering of circularly polarized light. Our results lead to the conclusion that the light-harvesting pigment-protein complex in thylakoid grana forms a helical macroarrav with dimensions commensurate with the wavelengths of the anomalous circular dichroism signals. A hypothesis is put forward suggesting a role for C i r c u l a r dichroism (CD)' is a powerful technique, yielding structural information on biological systems. CD of absorbance (CDA), an intrinsic property of chiral molecules, may also arise from short-or long-range coupling between chromophores of

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“…During the rotation of the chloroplast caused by linearly polarized high light intensity designed to present a smaller interface to the incoming light, the SHG increased considerably compared with the intergranal regions. Faludi-Daniel et al 1978, refer to the structural distinctness of the chloroplast regions. Sarafis, 1998, reviewed and showed the range of chloroplast structure in higher plants in both deep shade plant leaves and dry resurrection plant leaves indicating a large variation in granal size which is expected to be shown by future second harmonic studies of these chloroplasts.…”

Section: Discussionmentioning

confidence: 99%

“…The collecting efficiency of SHG signals is highly dependent on the numerical aperture (N.A.) of the objective Han et al, 2005;Pantic-Tanner and Eden, 1999;Faludi-Daniel et al, 1978;Sarafis, 1998). Additional infrared beam block filters (HQ700SP-2p 58398) were placed in front of each PMT to ensure that illumination light was effectively suppressed and only TPEF and SHG signals were recorded.…”

Section: Laser Sources and Imaging Systemmentioning

confidence: 99%

Second Harmonic Generation Signal from Biological Materials Using Multi-Functional Two-Photon Laser Scanning Microscopy

Reshak¹

2011

Laser Scanning, Theory and Applications

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Differential light-scattering of granal and agranal chloroplasts and their fragments

Cited by 10 publications

References 12 publications

Changes in leaf optical properties associated with light‐dependent chloroplast movements

Changes in leaf optical properties associated with light‐dependent chloroplast movements

Macroorganization of chlorophyll a/b light-harvesting complex in thylakoids and aggregates: information from circular differential scattering

Second Harmonic Generation Signal from Biological Materials Using Multi-Functional Two-Photon Laser Scanning Microscopy

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