Differential Lectin Binding to Microvascular Endothelial Glycoconjugates during Normal Angiogenesis in the Chick Chorioallantoic Membrane

Henry, Charmaine B. S.; DeFouw, David O.

doi:10.1006/mvre.1995.1016

Cited by 15 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The negatively charged glycocalyx also creates both a charge and a size barrier for protein diffusion to the endothelial cell surface and is vital for endothelial barrier function (Florian et al, 2003;Vink and Duling, 2000). Selective degradation of the adult glycocalyx has been shown to increase vascular permeability (Henry and DeFouw, 1995), cause attenuated nitrous oxide availability (Mochizuki et al, 2003), and increase adhesion of leukocytes and platelets to the endothelium (Constantinescu et al, 2003). Thinning of the glycocalyx has been linked to diseases such as diabetes mellitus, where dysfunction of the glycocalyx is believed to contribute to an increase in vascular permeability (Nieuwdorp et al, 2006a).…”

Section: Introductionmentioning

confidence: 98%

“…Lectins are proteins that bind to specific disaccharides in glycosaminoglycan chains (Barker et al, 2004;Florian et al, 2003;Mulivor and Lipowsky, 2004). Lectin binding affinities change during early vascular development (Henry and DeFouw, 1995) and therefore, there is indirect evidence that the composition of the glycocalyx is variable during development. Beyond this, not much is known about the presence of the glycocalyx in the embryo.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The glycocalyx is present as soon as blood flow is initiated and is required for normal vascular development

Henderson-Toth

Jahnsen

Jamarani

et al. 2012

Developmental Biology

View full text Add to dashboard Cite

The glycocalyx, and the thicker endothelial surface layer (ESL), are necessary both for endothelial barrier function and for sensing mechanical forces in the adult. The goal of this study is to use a combination of imaging techniques to establish when the glycocalyx and endothelial surface layer form during embryonic development and to determine the biological significance of the glycocalyx layer during vascular development in quail embryos. Using transmission electron microscopy, we show that the glycocalyx layer is present as soon as blood flow starts (14 somites). The early endothelial glycocalyx (14 somites) lacks the distinct hair-like morphology that is present later in development (17 and 25 somites). The average thickness does not change significantly (14 somites, 182 nm ± 33 nm; 17 somites, 218 ± 30 nm; 25 somites, 212 ± 32 nm). The trapping of circulating fluorescent albumin was used to evaluate the development of the ESL. Trapped fluorescent albumin was first observed at 25 somites. In order to assess a functional role for the glycocalyx during development, we selectively degraded luminal glycosaminoglycans. Degradation of hyaluronan compromised endothelial barrier function and prevented vascular remodeling. Degradation of heparan sulfate down regulated the expression of shear-sensitive genes but does not inhibit vascular remodeling. Our findings show that the glycocalyx layer is present as soon as blood flow starts (14 somites). Selective degradations of major glycocalyx components were shown to inhibit normal vascular development, examined through morphology, vascular barrier function, and gene expression.

show abstract

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The glycocalyx is present as soon as blood flow is initiated and is required for normal vascular development

Henderson-Toth

Jahnsen

Jamarani

et al. 2012

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…These authors have shown that GSA-I binds to capillaries in muscles of a number of mammalian species; more capillaries were detected by this method than by any other. In contrast to mammalian species, lectin staining of endothelium of blood vessels of chick and quail, two species widely used in experimental embryology, has so far only been infrequently used (Henry and DeFouw 1995;Nico et al 1998). We therefore tested a panel of selected lectins for their binding specificity to endothelium in prenatal and adult muscles of chick and quail.…”

Section: Introductionmentioning

confidence: 99%

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Naňka

Peumans

Damme

et al. 2001

Anatomy and Embryology

View full text Add to dashboard Cite

The lectin binding pattern of muscular microvessels in chick, quail and chick/quail chimeras was analysed. Paraffin wax sections of muscles from embryonic and adult animals were used. The biotin-labelled lectins were detected by avidin-alkaline phosphatase complex. The following lectins bound to muscular microvessels including arterioles, capillaries and venules of both species: SNA-I (Sambucus nigra agglutinin), MAA (Maackia amurensis agglutinin), AIA (Artocarpus integrifolia agglutinin), VAA-I, VAA-II and VAA-III (Viscum album agglutinin I-III), WGA (wheat germ agglutinin), LEA (Lycopersicon esculentum agglutinin). Endomysium and basement membranes of muscle fibres were also stained to a variable extent and intensity. Only SNA-I stained almost exclusively the endothelium of blood vessels. WFA (Wisteria floribunda agglutinin) bound to the quail endothelium only. MPA (Maclura pomifera agglutinin) marked vessels in adult muscles of chick and quail, but embryonic vessels were stained in quail only. Our results show that lectin histochemistry is a useful tool for visualisation of microvasculature in avian species. In particular, WFA and MPA can be used to determine the origin of endothelia in chick/quail chimeras.

show abstract

“…These (mostly) plant-derived proteins bind to specific sugar moieties. A wide variety of lectins is commercially available and can be used to investigate (endothelial) glycocalyx composition in different models [15,16,17,18]. When applying lectins to the EG, one should take into account both the specificity of the lectin and the abundance of its target in the EG.…”

Section: Introductionmentioning

confidence: 99%

Endothelial Glycocalyx Structure in the Intact Carotid Artery: A Two-Photon Laser Scanning Microscopy Study

Reitsma¹,

Egbrink

Vink

et al. 2011

J Vasc Res

View full text Add to dashboard Cite

Background: The endothelial glycocalyx (EG) is the carbohydrate-rich luminal lining of endothelial cells that mediates permeability and blood cell-vessel wall interactions. To establish an atheroprotective role of the EG, adequate imaging and quantification of its properties in intact, viable, atherogenesis-prone arteries is needed. Methods: Carotid arteries of C57Bl6/J mice (n = 22) were isolated including the bifurcation, mounted in a perfusion chamber, and perfused with fluorescent lectin wheat germ agglutinin-fluorescein isothiocyanate. The EG was visualized through the vessel wall using two-photon laser scanning microscopy. An image quantification protocol was developed to assess EG thickness, which was sensitive to hyaluronidase-induced changes. Results: In the lesion-protected common carotid artery, EG thickness was found to be 2.3 ± 0.1 µm (mean ± SEM), while the surface area devoid of (wheat germ agglutinin-sensitive) EG was 8.9 ± 4.2%. Data from the external carotid artery were similar (2.5 ± 0.1 µm; 9.1 ± 5.0%). In the atherogenesis-prone internal carotid artery the EG-devoid surface area was significantly higher (27.4 ± 5.5%, p < 0.05); thickness at the remaining areas was 2.5 ± 0.1 µm. Conclusion: The EG can be adequately imaged and quantified using two-photon laser scanning microscopy in intact, viable mounted carotid arteries. Spatial EG differences could underlie atherogenesis.

show abstract

Differential Lectin Binding to Microvascular Endothelial Glycoconjugates during Normal Angiogenesis in the Chick Chorioallantoic Membrane

Cited by 15 publications

References 0 publications

The glycocalyx is present as soon as blood flow is initiated and is required for normal vascular development

The glycocalyx is present as soon as blood flow is initiated and is required for normal vascular development

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Endothelial Glycocalyx Structure in the Intact Carotid Artery: A Two-Photon Laser Scanning Microscopy Study

Contact Info

Product

Resources

About