Differential labeling of the pinealocytes and pineal interstitial cells by a series of monoclonal antibodies to human pineal body

Nakazato, Yoichi; Hirato, Junko; Sasaki, Atsushi; Yokoo, Hideaki; Arai, Hanako; Yamane, Yuko; Sawai, Jyunki

doi:10.1046/j.0919-6544.2001.00423.x

Cited by 6 publications

(9 citation statements)

References 18 publications

(18 reference statements)

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“…All the antibodies (PP1, PP5, PP6) have been shown to be fairly specific for pinealocytes in normal human brain. 6 Positive reactivity of those antibodies is frequently seen in PPTs, and the antibody PP1 showed no positivity or positivity for only a small number of cells in pineoblastomas. 7 In the present case, almost half of the tumor cells were stained with PP1.…”

Section: Discussionmentioning

confidence: 98%

“…The immunoreactivity for a variety of neuronal markers (synaptophysin, NFP, NSE, MAP2, β‐tubulin III) and some pinealocyte‐associated antibodies (PP1, PP5, PP6) supports the diagnosis of PPT in the present case. All the antibodies (PP1, PP5, PP6) have been shown to be fairly specific for pinealocytes in normal human brain 6 . Positive reactivity of those antibodies is frequently seen in PPTs, and the antibody PP1 showed no positivity or positivity for only a small number of cells in pineoblastomas 7 .…”

Section: Discussionmentioning

confidence: 98%

“…Immunohistochemistry was performed using the biotin‐streptavidin immunoperoxidase method (Histofine kit, Nichirei, Tokyo, Japan). Specificity, sources and dilutions of the antibodies used were as follows: glial fibrillary acidic protein (GFAP, polyclonal, our own, 4 1:10 000), S‐100 protein (polyclonal, our own, 5 1:20 000), neurofilament protein (NFP, monoclonal NF‐M + H, Zymed, South San Francisco, USA, 1:5), synaptophysin (monoclonal SY38, Dako, Glostrup, Denmark, 1:10), chromogranin A (CGA, polyclonal, Dakopatts, Glostrup, Denmark, 1:1000), neuron‐specific enolase (NSE, monoclonal, Dako, 1:200), human paired helical filaments (PHF)‐tau (monoclonal AT8, Innogenetics, Gent, Belgium, 1:1000), pinealocyte‐associated antibodies PP1, PP5, PP6 (monoclonal, our own, 6,7 1:10, 1:800, 1:200, respectively), alpha‐fetoprotein (AFP, polyclonal, Dako, 1:2000), human chorionic gonadotropin (HCG, polyclonal, Dako, 1:300), MAP2 (monoclonal HM‐2, Sigma, St. Louis, MO, USA, 1:100), antiβ‐tubulin III (monoclonal, Tuj1, Covance, Richmond, CA, USA, 1:1500) and anti‐Ki67 (monoclonal, MIB‐1, Immunotech, Marseille, France, 1:50).…”

Section: Case Studymentioning

confidence: 99%

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Pineal parenchymal tumor of intermediate differentiation with cytologic pleomorphism

Sasaki¹,

Horiguchi

Nakazato³

2006

Neuropathology

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We report a case of pineal parenchymal tumor in a 33-year-old man incidentally detected by radiological examination. The MRI showed an unhomogeneously enhanced, small tumor (approximately 1 cm in size) in the pineal region. A tumor specimen was obtained at endoscopic biopsy. Routine histology showed a highly cellular tumor characterized by a predominance of small cells showing high nuclear : cytoplasmic ratio and moderate nuclear atypia, pleomorphism including giant cells and an absence of pineocytomatous rosettes. Mitotic figures were rare (approximately 1 per 10 high-power fields). Tumor necrosis was not evident. Immunohistochemically, the neoplastic cells showed positivity for neural markers (neurofilament protein, synaptophysin) and pinealocyte-associated antibodies (PP1, PP5, PP6), but not for glial fibrillary acidic protein or S-100. The MIB-1 labeling index was relatively high (6.3%). Ultrastructurally, there was some evidence of pinealocytic differentiation, such as vesicle-crowned rodlets (synaptic ribbons) and paired twisted filaments in neoplastic cells. Thus, the tumor was confirmed as a pineal parenchymal tumor of intermediate differentiation by histology, immunohistochemistry and electron microscopy. This case indicates that marked cytologic pleomorphism can occur in pineal parenchymal tumors of intermediate differentiation.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Case Studymentioning

confidence: 99%

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Pineal parenchymal tumor of intermediate differentiation with cytologic pleomorphism

Sasaki¹,

Horiguchi

Nakazato³

2006

Neuropathology

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“…Ten monoclonal antibodies against pineal cells were established in our laboratory. 15 Sections of 3 µm thickness were cut from formalin-fixed and paraffin-embedded tissues.The sections were dewaxed, rehydrated, treated with 0.3% H 2 O 2 in methanol for 30 min to inhibit endogenous peroxidase activity, rinsed in phosphate buffered saline (PBS) and incubated with 10% normal goat or rabbit serum for 30 min to block non-specific antibody binding. The sections were then overlaid with optimally diluted antibodies in a moist chamber, and incubated overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical characterization of pineal parenchymal tumors using novel monoclonal antibodies to the pineal body

2002

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To characterize the immunohistochemical nature of pineal parenchymal tumors (PPT), we examined nine cases of normal pineal bodies and 23 cases of PPT using several neuronal and glial antibodies and 10 novel monoclonal antibodies raised against human pineal tissue. The PPT were classified into four pineocytoma, five pineal parenchymal tumor of intermediate differentiation (Int-PPT), and 14 pineoblastoma. The pinealocytes, parenchymal cells of the pineal body, were labeled with five, neuronal and seven pineal monoclonal (from PP1 to PP7) antibodies in the normal pineal bodies. The subjects ranged from 3 to 85 years old, 12 female and eight male subjects were studied. Antibodies to glial cells PI1, PI2 and PX1, stained interstitial cells of the pineal body. Many of the PPT showed positive immunostaining for pinealocyte-associated antigens and neuronal markers. The intensity of immunostaining showed some association with the degree of differentiation of the tumor, but there was a considerable variety of staining from case to case. The pineocytomas are more immunopositive than are the Int-PPT or pineoblastoma for neuronal and pinealocyte-associated antibodies. In particular the neurofilament protein (NFP)68 kDa, PP1 and PP6 showed significant differences of reactivity between pineocytoma, Int-PPT and pineoblastoma, when compared in groups showing extensive positive staining (positive staining in almost all areas of the tumor). By using three representative antibodies, anti-NFP68kDa, PP1 and PP6, we were able to make a clear distinction between pineoblastoma, Int-PPT and pineocytoma. Glial fibrillary acidic protein (GFAP), PI1 and PI2 antibodies only occasionally showed a small number of positive cells in the tumor, and thus we considered these cells to be non-neoplastic interstitial cells or reactive astrocytes entrapped in the tumor. Our data suggest that the glial differentiation of PPT may occur, but that it seems to be a very rare event.

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“…From these histological and immuohistochemical results, we made a final diagnosis of PPTID. 4 In the current WHO classification, PPTID includes pineocytoma with anaplasia and mixed pineocytoma and pineoblastoma. In the former case, tumor cells morphologically exhibit malignant features, but the biological and clinical behavior is similar to benign pineocytoma.…”

Section: Discussionmentioning

confidence: 99%

Cytologic feature by squash preparation of pineal parenchyma tumor of intermediate differentiation

Shimada

Nakamura

Kuga

et al. 2008

Diagnostic Cytopathology

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Pineal parenchyma tumor of intermediate differentiation (PPTID) is a very rare intracranial tumor, and pathological investigation limited to immunohistological and ultrastructural analyses have been published to date. Although intraoperative cytology is one of the important approaches for initial diagnosis in brain tumors, no or little studies on cellular morphology of PPTID have been demonstrated due to its rarity. We report here cytological features of PPTID obtained from stereotactic surgical specimens in a case of 27-year-old female manifested by dizziness and diplopia. Brain MRI revealed an unhomogeneously enhanced, large-sized tumor (56 x 52 x 60 mm) mainly located in the pineal region expanding from the midbrain to superior portion of the cerebellum and the fourth ventricle. Squash cytology showed increased nucleocytoplasmic ratio, hyperchromatic nuclei, and small rosette-like cell cluster but cellular pleomorphism was mild to moderate and necrotic background was not observed. Histology showed high cellularity, moderate nuclear atypia, and small rosette formation but neither bizarre tumor cells nor necrosis was present. Mitotic counts were very low (less than 1 per 10 high-power fields) and the MIB-1 labeling index was relatively high (10.1%). Tumor cells were immunohistochemically positive for neural markers such as synaptophysin, neurospecific enolase but not for glial fibrillary acidic protein or S-100. In some parts, cells were strongly reactive for neurofilament protein. Taken together, we made a final diagnosis of PPTID. This is the first presentation of cytological analysis by squash preparation that gives an important clue to accurate diagnosis of pineal parenchymal tumor and to understand its malignant potential.

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Differential labeling of the pinealocytes and pineal interstitial cells by a series of monoclonal antibodies to human pineal body

Cited by 6 publications

References 18 publications

Pineal parenchymal tumor of intermediate differentiation with cytologic pleomorphism

Pineal parenchymal tumor of intermediate differentiation with cytologic pleomorphism

Immunohistochemical characterization of pineal parenchymal tumors using novel monoclonal antibodies to the pineal body

Cytologic feature by squash preparation of pineal parenchyma tumor of intermediate differentiation

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