Differential kinetics at PK/PEPCK branch point in the cestode, Raillietina echinobothrida

Das, Bidyadhar; Ramnath,; Dutta, Asim Kumar; Tandon, Veena

doi:10.1016/j.exppara.2015.03.023

Cited by 6 publications

(8 citation statements)

References 46 publications

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“…It is made In cestodes, glucose oxidation leads either to the formation of lactate, or to the formation of oxaloacetate, and then malate, which is converted into succinate and acetate in mitochondria (CO 2 fixation pathway; malate dismutation pathway) (Smyth and McManus, 2007;Ritler et al, 2019). This cleavage occurs at the level of phosphoenolpyruvate, for which pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) compete (Das et al, 2015) (Fig. 4).…”

Section: Discussionmentioning

confidence: 99%

Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host

Borvinskaya

Kochneva

Drozdova

et al. 2021

Preprint

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The protein composition (proteome) of cestode Schistocephalus solidus was measured in an experiment simulating the transition of the parasite from a cold-blooded to a warm-blooded host. Infective S. solidus plerocercoids obtained from the three-spined stickleback Gasterosteus aculeatus were heated at 40 °C for 1 hour or cultured in vitro at 40 °C and 22 °C for 48 hours. In short-term experiment, the content of only one tegument protein was evidenced to decrease after heating. After long-term heating, which triggered parasite sexual maturation, an increase in the content of ribosomal proteins, translation initiation factors and enzymes of the amino acid biosynthesis pathway was observed. The synthesis of certain gene products for carbohydrate metabolism, including glycolysis/gluconeogenesis, was found to be regulated in parasite by temperature.

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Section: Discussionmentioning

confidence: 99%

Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host

Borvinskaya

Kochneva

Drozdova

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In cestodes, glucose oxidation leads either to the formation of lactate, or to the formation of oxaloacetate, and then malate, which is converted into succinate and acetate in mitochondria (CO 2 fixation pathway; malate dismutation pathway) (Smyth and McManus, 2007;Ritler et al, 2019). This cleavage occurs at the level of phosphoenolpyruvate, for which pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) compete (Das et al, 2015) (Fig. 4).…”

Section: Discussion Proteomic Changes In S Solidis Are Mostly Energy-relatedmentioning

confidence: 99%

Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host

et al. 2021

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The protein composition of the cestode Schistocephalus solidus was measured in an experiment simulating the trophic transmission of the parasite from a cold-blooded to a warm-blooded host. The first hour of host colonisation was studied in a model experiment, in which sticklebacks Gasterosteus aculeatus infected with S. solidus were heated at 40°C for 1 h. As a result, a decrease in the content of one tegument protein was detected in the plerocercoids of S. solidus. Sexual maturation of the parasites was initiated in an experiment where S. solidus larvae were taken from fish and cultured in vitro at 40°C for 48 h. Temperature-independent changes in the parasite proteome were investigated by incubating plerocercoids at 22°C for 48 h in culture medium. Analysis of the proteome allowed us to distinguish the temperature-induced genes of S. solidus, as well as to specify the molecular markers of the plerocercoid and adult worms. The main conclusion of the study is that the key enzymes of long-term metabolic changes (glycogen consumption, protein production, etc.) in parasites during colonisation of a warm-blooded host are induced by temperature.

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“…Further, Ki of these compounds for PK and PEPCK from the cestode parasite has been determined. Ki for genistein is 0.26 and 0.17 lM for PK and PEPCK enzymes from the parasite, respectively; whereas, Ki for daidzein is 0.19 and 0.29 lM, respectively (Das et al 2015).…”

Section: Effect Of Genistein On Carbohydrate Metabolismmentioning

confidence: 94%

Genistein: is the multifarious botanical a natural anthelmintic too?

Tandon

Das

2018

J Parasit Dis

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Genistein (4',5,7-trihydroxyisoflavone) is naturally present in plants of the soy family and is known to have various pharmacological activities, such as anti-cancer, anti-diabetic, anti-oxidant, etc. The phytoestrogen is one of the major isoflavones found in some medicinal plants having anthelmintic properties. This review describes the putative role of genistein as an anthelmintic, which has been tested on some helminth parasites in vitro. Genistein has been shown to cause paralysis and alterations in the tegument and tegumental enzymes (acid phosphatase, alkaline phosphatase, adenosine triphosphatase, and 5'-nucleotidase) of helminth parasites. Alterations in the activities of several enzymes associated with the coordination system (specifically non-specific esterases, acetylcholine esterase, and nitric oxide synthase), and changes in the concentration of nitric oxide, cGMP, free amino acid pool, and tissue ammonia are observed in helminth parasites treated with genistein. The phytoestrogen also affects the carbohydrate metabolism by altering the activities of key enzymes involved in glycogen- and glucose-metabolism of a cestode parasite. Considering the significance of phosphoenolpyruvate carboxykinase (PEPCK) in glycolysis of the cestode parasite, i of the phytoestrogen for PEPCK in the parasite has been determined, and molecular docking of genistein into the active site of the enzyme has also been described. The potential beneficial role of genistein as a natural alternative in management of helminth parasites needs to be further explored, particularly considering its in vivo efficacy and pharmacokinetics.

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Differential kinetics at PK/PEPCK branch point in the cestode, Raillietina echinobothrida

Cited by 6 publications

References 46 publications

Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host

Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host

Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host

Genistein: is the multifarious botanical a natural anthelmintic too?

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