Differential Interference Contrast Microscopy (DIC)

Shribak, Michael

doi:10.1016/b978-0-12-415871-9.00002-8

Cited by 4 publications

(8 citation statements)

References 32 publications

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“…In this section, a brief review of our previously published works about the quantitative orientation-independent differential interference contrast (OI-DIC) microscopy [6][7][8][21][22][23] with updates is presented for completeness. The optical scheme of the OI-DIC assembly, which allows changing the shear direction, is shown in Fig.…”

Section: Optical Set-up Of the Orientation-independent Dic Microscopementioning

confidence: 99%

“…Earlier, Shribak described two simple methods for determining the shear angle by measuring the retardance variation of a single DIC prism in the transmitted or in the reflected light and tabulating the shear distance produced by standard Olympus DIC prisms with various objective lenses. 6,7 The shear distance is a product of the focal distance of the objective lens and the shear angle of the DIC prism.…”

Section: Mapping the Optical Path Length Gradient Vectormentioning

confidence: 99%

“…The DIC image is produced by interference of the light field coming from the object with a laterally displaced copy of itself. [1][2][3][4][5][6] The DIC microscope can employ a high numerical aperture (NA) objective and condenser lenses and, therefore, provides good lateral resolution as well as good axial discrimination. The shear distance is usually smaller than the Airy disk radius, 7 and it does not affect the lateral resolution substantially.…”

Section: Introductionmentioning

confidence: 99%

“…18 This is double the shear of a standard high-resolution Olympus Nomarski DIC prism U-DICTH. 6 In addition, the microscope suffers from light energy loss. It only uses the first diffraction orders.…”

Section: Introductionmentioning

confidence: 99%

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Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy

Shribak

Larkin²,

Biggs

2017

J. Biomed. Opt

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We describe the principles of using orientation-independent differential interference contrast (OI-DIC) microscopy for mapping optical path length (OPL). Computation of the scalar two-dimensional OPL map is based on an experimentally received map of the OPL gradient vector field. Two methods of contrast enhancement for the OPL image, which reveal hardly visible structures and organelles, are presented. The results obtained can be used for reconstruction of a volume image. We have confirmed that a standard research grade light microscope equipped with the OI-DIC and 100 × / 1.3 NA objective lens, which was not specially selected for minimum wavefront and polarization aberrations, provides OPL noise level of ? 0.5 ?? nm and lateral resolution if ? 300 ?? nm at a wavelength of 546 nm. The new technology is the next step in the development of the DIC microscopy. It can replace standard DIC prisms on existing commercial microscope systems without modification. This will allow biological researchers that already have microscopy setups to expand the performance of their systems.

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Section: Optical Set-up Of the Orientation-independent Dic Microscopementioning

confidence: 99%

Section: Mapping the Optical Path Length Gradient Vectormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy

Shribak

Larkin²,

Biggs

2017

J. Biomed. Opt

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“…The differential interference contrast (DIC) microscope is the most suitable instrument for imaging transparent animals (Shribak, 2012). However the contrast in conventional DIC imaging depends on the specimen orientation.…”

Section: Introductionmentioning

confidence: 99%

An orientation‐independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model

Malamy

Shribak

2018

Journal of Microscopy

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Epithelial cell dynamics can be difficult to study in intact animals or tissues. Here we use the medusa form of the hydrozoan Clytia hemisphaerica, which is covered with a monolayer of epithelial cells, to test the efficacy of an orientation-independent differential interference contrast (OI-DIC) microscope for in vivo imaging of wound healing. OI-DIC provides an unprecedented resolution phase image of epithelial cells closing a wound in a live, non-transgenic animal model. In particular, the OI-DIC microscope equipped with a 40×/0.75NA objective lens and using the illumination light with wavelength 546 nm demonstrated a resolution of 460 nm. The repair of individual cells, the adhesion of cells to close a gap, and the concomitant contraction of these cells during closure is clearly visualized.

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Differential interference contrast microscopy with adjustable plastic Sanderson prisms

Rathi¹,

Zoubek²,

Zagarese³

et al. 2020

Appl. Opt.

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Differential interference contrast (DIC) microscopy is a technique to image spatially dependent gradients in optical path lengths. Contrast is produced through the splitting of polarized light with quartz Wollaston prisms. Here we demonstrate that light splitting for DIC microscopy can also be achieved with Sanderson prisms consisting of polycarbonate bars under a bending load. Comparable image contrast while imaging cultured cells was achieved with this alternative technique. These results demonstrate an inexpensive and easily adjustable alternative to traditional quartz Wollaston prisms.

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Differential Interference Contrast Microscopy (DIC)

Cited by 4 publications

References 32 publications

Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy

Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy

An orientation‐independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model

Differential interference contrast microscopy with adjustable plastic Sanderson prisms

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