Differential interactions of the antimicrobial peptide, RQ18, with phospholipids and cholesterol modulate its selectivity for microorganism membranes

Almeida, Claudiane Vilharroel; Oliveira, Caio Fernando Ramalho de; Santos, Edson Lucas dos; Santos, Helder Freitas dos; Júnior, Edson Crusca; Marchetto, Reinaldo; Cruz, Letícia Alves da; Ferreira, Alda Maria Teixeira; Gomes, Valdirene Moreira; Taveira, Gabriel Bonan; Costa, Bruna O.; Franco, Octávio Luiz; Cardoso, Marlon Henrique; Macedo, Maria Lı́gia Rodrigues

doi:10.1016/j.bbagen.2021.129937

Cited by 12 publications

(11 citation statements)

References 78 publications

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“…To investigate the time of action of the Ca-MAP1 peptide, a membrane permeability assay with the dye Sytox Green was performed with E. coli KPC bacteria treated with the peptide at a concentration 30 times higher than the minimum inhibitory concentration [ 19 , 20 ]. The analyses show that in 15 min 84% of bacterial membrane permeabilization already occurs, and in 20 min Ca-MAP1 causes 98% permeabilization, as shown in Figure 3 .…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin

Oliveira

Boleti

Silva

et al. 2022

Toxins

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The importance of neuroinflammation in neurology is becoming increasingly apparent. In addition to neuroinflammatory diseases such as multiple sclerosis, the role of neuroinflammation has been identified in many non-inflammatory neurological disorders such as stroke, epilepsy, and cancer. The immune response within the brain involves the presence of CNS resident cells; mainly glial cells, such as microglia, the CNS resident macrophages. We evaluated the peptide Ca-MAP1 bioinspired on the C. albicans immature cytolytic toxin candidalysin to develop a less hemolytic peptide with anti-neuroinflammatory, antibacterial, and cytotoxic activity against tumor cells. In silico and in vitro studies were performed at various concentrations. Ca-MAP1 exhibits low hemolytic activity at lower concentrations and was not cytotoxic to MRC-5 and BV-2 cells. Ca-MAP1 showed activity against Acinetobacter baumannii, Escherichia coli ATCC, E. coli KPC, Klebsiella pneumoniae ATCC, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC. Furthermore, Ca-MAP1 exhibits anti-neuroinflammatory activity in the BV-2 microglia model, with 93.78% inhibition of nitrate production at 18.1 µM. Ca-MAP1 presents cytotoxic activity against tumor cell line NCI-H292 at 36.3 μM, with an IC50 of 38.4 µM. Ca-MAP1 demonstrates results that qualify it to be evaluated in the next steps to promote the control of infections and provide an alternative antitumor therapy.

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Section: Resultsmentioning

confidence: 99%

“…Membrane permeability was investigated as described by Mohanram and Bhattacharjya (2016) [ 20 ] and modifications proposed by Almeida et al (2021) [ 19 ]. In the assay, a suspension of E. coli KPC + 001812446, grown in MH broth for 18 h at 37 °C, was prepared with OD 600 nm at 0.5 in 10 mM sodium phosphate buffer, pH 7.0.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin

Oliveira

Boleti

Silva

et al. 2022

Toxins

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“…The antimicrobial activity of peptides was evaluated by analysis the growth of E. coli (ATCC 43895), S. aureus (ATCC 14458) and C. albicans (ATCC 90028), in a microdilution test [7]. The MIC values were considered as the lowest concentration of peptide able to inhibit at least 90% of the strains growth, within 24 hours for the bacteria and 48 hours for the fungus.…”

Section: Resultsmentioning

confidence: 99%

Peptide Dimerization Effect on Bacterial Topoisomerases Activity

Roch¹,

Angelo²,

Crusca³

et al. 2022

Proceedings of the 36th European and the 12th International Peptide Symposium

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“…AMPs emerged as potential candidates for the treatment of microbial infections [ 40 ]. Therefore, investigating their activity in conditions similar to clinical application, in addition to antimicrobial and hemolytic trials, is crucial for greater knowledge about the structure, design, and activity.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa

Silva

Guindo

Oliveira

et al. 2023

Toxins

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In recent years, antimicrobial peptides isolated from amphibian toxins have gained attention as new multifunctional drugs interacting with different molecular targets. We aimed to rationally design a new peptide from temporin-PTa. Hp-MAP3 (NH2-LLKKVLALLKKVL-COOH), net charge (+4), hydrophobicity (0.69), the content of hydrophobic residues (69%), and hydrophobic moment (0.73). For the construction of the analog peptide, the physicochemical characteristics were reorganized into hydrophilic and hydrophobic residues with the addition of lysines and leucines. The minimum inhibitory concentration was 2.7 to 43 μM against the growth of Gram-negative and positive bacteria, and the potential for biofilm eradication was 173.2 μM. Within 20 min, the peptide Hp-MAP3 (10.8 μM) prompted 100% of the damage to E. coli cells. At 43.3 μM, eliminated 100% of S. aureus within 5 min. The effects against yeast species of the Candida genus ranged from 5.4 to 86.6 μM. Hp-MAP3 presents cytotoxic activity against tumor HeLa at a concentration of 21.6 μM with an IC50 of 10.4 µM. Furthermore, the peptide showed hemolytic activity against murine erythrocytes. Structural studies carried out by circular dichroism showed that Hp-MAP3, while in the presence of 50% trifluoroethanol or SDS, an α-helix secondary structure. Finally, Amphipathic Hp-MAP3 building an important model for the design of new multifunctional molecules.

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Differential interactions of the antimicrobial peptide, RQ18, with phospholipids and cholesterol modulate its selectivity for microorganism membranes

Cited by 12 publications

References 78 publications

Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin

Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin

Peptide Dimerization Effect on Bacterial Topoisomerases Activity

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa

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