Differential interaction of<i>Phytophthora infestans</i>on tubers of potato cultivars with different levels of blight resistance

Flier, W.G.; Turkensteen, L. J.; Bosch, G.B.M. van den; Vereijken, P.H.; Mulder, A.

doi:10.1046/j.1365-3059.2001.00574.x

Cited by 35 publications

(42 citation statements)

References 34 publications

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“…Expression of tuber resistance depends on the tissue inoculated (Jwieryjski and Zimnoch-Guzowska 2001). The tuber skin and the cambial layer just underneath the tuber skin can be important barriers for infection (Toxopeus 1958(Toxopeus , 1961, but the periderm, cortex and medulla can also be involved in tuber resistance (Flier et al 2001). Recently, Park et al (2005c) showed that the genes Rpi-abpt and R3a are foliage-speciWc, while R1 or R1-like is eVective both in tubers and foliage, conWrming previous Wndings of Roer and Toxopeus (1961), who also investigated the eVects of R1 and R3 on tuber resistance.…”

Section: Discussionsupporting

confidence: 53%

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

et al. 2006

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Despite the long history of breeding potatoes resistant to Phytophthora infestans, this oomycete is still economically the most important pathogen of potato worldwide. The correlation of high levels of resistance to late blight with a long vegetation period is one of the bottlenecks for progress in breeding resistant cultivars of various maturity types. Solanum phureja was identiWed as a source of eVective late blight resistance, which was transferred to the cultivated gene pool by interspeciWc crosses with dihaploids of Solanum tuberosum. A novel major resistance locus, Rpiphu1, derived most likely from S. phureja and conferring broad-spectrum resistance to late blight, was mapped to potato chromosome IX, 6.4 cM proximal to the marker GP94. Rpi-phu1 was highly eVective in detached leaXet, tuber slice and whole tuber tests during 5 years of quantitative phenotypic assessment. The resistance did not show signiWcant correlation with vegetation period length. Our Wndings provide a well-characterized new source of resistance for breeding early and resistant-to-P. infestans potatoes.

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Section: Discussionsupporting

confidence: 53%

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

et al. 2006

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“…The tuber skin and the layer just below the tuber skin (inoculated in whole tuber tests) form two diVerent, important barriers for the late blight infection (Toxopeus 1958(Toxopeus , 1961. However, cortex and medulla (inoculated in slice tests) can also be involved in resistance mechanism (Flier et al 2001). Three resistance components: leaXet, slice and whole tuber resistance showed normal distribution in population 98-21 ( Fig.…”

Section: Discussionmentioning

confidence: 98%

Tagging QTLs for late blight resistance and plant maturity from diploid wild relatives in a cultivated potato (Solanum tuberosum) background

et al. 2007

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Phytophthora infestans causes an economically important disease of potato called late blight. The epidemic is controlled chemically but resistant potatoes can become an environment-friendly and Wnancially justiWed alternative solution. The use of diploid Solanum tuberosum derived from European tetraploid cultivars enabled the introgression of novel genes encoding foliage resistance and tuber resistance from other species into the modern cultivated potato gene pool. This study evaluated the resistance of the obtained hybrids, its quality, expression in leaXets and tubers and its relation to the length of vegetation period. We also identiWed genetic loci involved in late blight resistance and the length of vegetation period. A family of 156 individuals segregating for resistance to late blight was assessed by three laboratory methods: detached leaXet, tuber slice and whole tuber test, repeatedly over 5 years. Length of vegetation period was estimated by a Weld test over 2 years. The phenotypic distributions of all traits were close to normal. Using sequence-speciWc PCR markers of known chromosomal position on the potato genetic map, six quantitative trait loci (QTLs) for resistance and length of vegetation period were identiWed. The most signiWcant and robust QTL were located on chromosomes III (explaining 17.3% of variance observed in whole tuber tests), IV (15.5% of variance observed in slice tests), X (15.6% of variance observed in leaXet tests) and V (19.9% of variance observed in length of vegetation period). Genetic characterization of these novel resistance sources can be valuable for potato breeders and the knowledge that the most prominent QTLs for resistance and vegetation period length do not overlap in this material is promising with respect to breeding early potatoes resistant to P. infestans.

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“…Three major components are known to contribute to late blight resistance in tubers; 1) a physical barrier consisting of several layers of phloem cells, known the periderm; 2) the outer cortical cell layers that retard the growth of lesions and can completely block hyphal growth; and 3) medulla storage tissues which can reduce hyphal growth and sporulation of P. infestans (Flier,Turkensteen, & Mulder, 1998;Flier, Turkensteen,Van den Bosch, Vereijken, & Mulder, 2001;Pathak & Clarke, 1987). Furthermore, immature tubers are more susceptible to tuber blight than mature tubers (Grinberger, Kadish, & Cohen, 1995), which means that assayed tubers should be of similar age.…”

Section: Discussionmentioning

confidence: 99%

Determination of Resistance to Phytophthora infestans on Potato Plants in Field, Laboratory and Greenhouse Conditions

Sharma¹,

Forbes²,

Manandhar³

et al. 2013

JAS

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An experiment was conducted to determine the host resistance of potato against Phytophthora infestans for twenty-five potato genotypes in 2010 and 2011 at Khumaltar, Lalitpur, Nepal using four assays: three for foliage resistance (field, whole-plant and detached leaf) and one for tuber resistance (tuber slice). An isolate of P. infestans collected from Lalitpur (LPR-1) was used for inoculation at a concentration of 3 x 10 3 sporangia ml -1 in all assays. Infected foliage area in the field and whole-plant assays, lesion size on detached leaves, and colony growth on tuber slice were all individually converted to 0-9 interval scale for susceptibility. Field assessment, considering the most robust measure of resistance, was used as benchmark for comparing the other assays. Sixteen of the genotypes had very little disease in the field (scale value <1) indicating they were probably expressing race-specific resistance, which has historically been short lived. Susceptibility levels measured in the whole-pant assay were highly correlated (r = 0.90) with converted field scale values, although the correlation was lower for the detached leaf assay (r = 0.63) and least for tuber-slice assay (r = 0.46). Low correlation in the detached leaf assay was assumed to represent lower resolution of the single-cycle assay. Low correlation in the tuber assay may have also reflected genetic differences as foliage and tuber blight resistance are not always correlated. Genotypes with extreme resistance in the field were frequently identified as having partial resistance in the other assays, which could mistakenly be interpreted as more durable field resistance. The consequences for selecting durable resistance are discussed.

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Differential interaction ofPhytophthora infestanson tubers of potato cultivars with different levels of blight resistance

Cited by 35 publications

References 34 publications

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

Tagging QTLs for late blight resistance and plant maturity from diploid wild relatives in a cultivated potato (Solanum tuberosum) background

Determination of Resistance to Phytophthora infestans on Potato Plants in Field, Laboratory and Greenhouse Conditions

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