Differential inhibition of rat and human hepatic cytochrome P450 by Andrographis paniculata extract and andrographolide

Beneficial effects of anAndrographis paniculataextract and andrographolide on cognitive functions in streptozotocin-induced diabetic rats

“…Andrographolide and diverse other extractable secondary metabolites of A. paniculata possess bactericidal, fungicidal and antiviral activities (Sareer et al 2012), and oral bioavailability of andrographolide (as judged by its observed blood levels after oral intake) is low (Panossian et al 2000;Ye et al 2011). Andrographolide modulates physiological functions of diverse drug metabolising enzymes in liver and other peripheral organs as well (Pekthong et al 2008), and binds covalently with cellular metabolites and enzymes (Xia et al 2004). Andrographolide-lipoic acid conjugate possesses andrographolide like and longer lasting antidiabetic activity in mice (Zhang et al 2009).…”

Biological effects ofByrsocarpus coccineus in vitro

“…The fluorogenic substrates benzyloxyresorufin (BR) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC), selective for CYP2B1/2 and CYP3A4, respectively (Pekthong et al, 2008;Huber et al, 2008;Stresser et al, 2000), were synthesized from the reactions of resorufin or trifluoromethylcoumarin with benzyl iodide (Prough et al, 1978) and their identities were verified by 1 H NMR. BR and BFC were purified to >99% pure as evaluated by HPLC.…”

“…In African countries and developing countries of other continents of the world, self-medication and combination therapy using western medicines and traditional herbal medicines are frequent (Agbonon et al, 2010), based on personal conviction of synergistic or additive pharmacological effect (Pekthong et al, 2008). Patients often do not report herbal and alternative medicine use to physicians (Shakeel et al, 2010), but there are a number of examples where interactions between herbal medicines and drugs can be clinically significant.…”

Effect of Andrographolide Co-Administration on Pharmacokinetics of Meloxicam in Rats

“…However, no significant effect of andrographolide on pharmacokinetics of warfarin (Hovhannisyan et al, 2006) and midazolam (Wongnawa et al, 2012) was observed. Alteration in pharmacokinetic of meloxicam may be due to metabolism of meloxicam caused by major extent through CYP2C9 and, to a much lesser extent, CYP3A4 (Schmid et al, 1995;Chesné et al, 1998) and andrographolide was also found to modulate expression of CYP2C9 and CYP3A4 microsomal enzyme (Pekthong et al, 2008;Pekthong et al, 2009).…”

“…Moreover, it is reported that andrographolide modulate expression of several cytochromes P450 isoenzymes like CYP2C9, CYP3A4, CYP2C6/11, CYP1A1/2 and CYP3A1/2. (Chesné et al, 1998;Schmidet al, 1995;Pekthong et al, 2008; The pharmacokinetic of meloxicam (5 mg/kg) and effect of co-administration of andrographolide (50 mg/kg) on pharmacokinetics of meloxicam were studied following intramuscular administration in rats. Drug concentration in plasma was determined using High Performance Liquid Chromatography (HPLC).…”