Differential induction of CYP1A1 and CYP1B1 by benzo[a]pyrene in oral squamous cell carcinoma cell lines and by tobacco smoking in oral mucosa

Angela, C.; Appleton, Kathryn M.; Henriod, Joel B.; Krayer, Joe W.; Marlow, Nicole M.; Bandyopadhyay, Dipankar; Sigmon, Ryan C.; Kurtz, David T.

doi:10.1016/j.oraloncology.2009.05.562

Cited by 33 publications

(27 citation statements)

References 32 publications

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“…Elevated CYP1A1 and CYP1B1 mRNA expression has been shown in extrahepatic tissues from smokers and in human cell lines treated with cigarette smoke condensate or the cigarette smoke component, benzo[a]pyrene (Buchthal et al, 1995;Chi et al, 2009;Hakkola et al, 1997;Iwanari et al, 2002;Nagaraj et al, 2006;Thum et al, 2006). In the current study, while no statistically significant differences were evident, a trend toward induction was observed for CYP1A1 (in PFC and AMG) and CYP1B1 (in AMG) in samples from smokers, with two AS individuals showing particularly high CYP1A1 expression levels.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of cytochromes P450, ABC transporters and their principal transcription factors in the amygdala and prefrontal cortex of alcoholics, smokers and drug-free controls by qRT-PCR

et al. 2015

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1. Ethanol consumption and smoking alter the expression of certain drug-metabolizing enzymes and transporters, potentially influencing the tissue-specific effects of xenobiotics. 2. Amygdala (AMG) and prefrontal cortex (PFC) are brain regions that modulate the effects of alcohol and smoking, yet little is known about the expression of cytochrome P450 enzymes (P450s) and ATP-binding cassette (ABC) transporters in these tissues. 3. Here, we describe the first study on the expression of 19 P450s, their redox partners, three ABC transporters and four related transcription factors in the AMG and PFC of smokers and alcoholics by quantitative RT-PCR. 4. CYP1A1, CYP1B1, CYP2B6, CYP2C8, CYP2C18, CYP2D6, CYP2E1, CYP2J2, CYP2S1, CYP2U1, CYP4X1, CYP46, adrenodoxin and NADPH-P450 reductase, ABCB1, ABCG2, ABCA1, and transcription factors aryl hydrocarbon receptor AhR and proliferator-activated receptor α were quantified in both areas. CYP2A6, CYP2C9, CYP2C19, CYP3A4, CYP3A5, adrenodoxin reductase and the nuclear receptors pregnane X receptor and constitutive androstane receptor were detected but below the limit of quantification. CYP1A2 and CYP2W1 were not detected. 5. Adrenodoxin expression was elevated in all case groups over controls, and smokers showed a trend toward higher CYP1A1 and CYP1B1 expression. 6. Our study shows that most xenobiotic-metabolizing P450s and associated redox partners, transporters and transcription factors are expressed in human AMG and PFC.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling of cytochromes P450, ABC transporters and their principal transcription factors in the amygdala and prefrontal cortex of alcoholics, smokers and drug-free controls by qRT-PCR

et al. 2015

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“…In addition, CYP2A6 and CYP2A13 are involved in the metabolic activation of tobacco-specific nitrosamines into reactive compounds that lead to liver and lungs toxicities [44]. Other tobacco constituents, such as polyarylhydrocarbons (PAH) are mainly activated by CYP1A1 and CYP1B1 [45, 46] that leads to the formation of recative metabolites, which has been shown to be associated with oral toxicity [46]. Furthermore, a study comprising 600 patients has demonstrated that CYP1A1 is a risk factor in oral cancer among smokers [45].…”

Section: Mechanism Of Tobacco Smoking Mediated Toxicity: Role Of Cypmentioning

confidence: 99%

Tobacco smoking effect on HIV-1 pathogenesis: role of cytochrome P450 isozymes

Ande

McArthur

Kumar

et al. 2013

Expert Opinion on Drug Metabolism & Toxicology

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Introduction Tobacco smoking is highly prevalent among the HIV-1-infected population. In addition to diminished immune response, smoking has been shown to increase HIV-1 replication and decrease response to antiretroviral therapy, perhaps through drug–drug interaction. However, the mechanism by which tobacco/nicotine increases HIV-1 replication and mediates drug–drug interaction is poorly understood. Areas covered In this review, the authors discuss the effects of smoking on HIV-1 pathogenesis. Since they propose a role for the cytochrome P450 (CYP) pathway in smoking-mediated HIV-1 pathogenesis, the authors briefly converse the role of CYP enzymes in tobacco-mediated oxidative stress and toxicity. Finally, the authors focus on the role of CYP enzymes, especially CYP2A6, in tobacco/nicotine metabolism and oxidative stress in HIV-1 model systems monocytes/macrophages, lymphocytes, astrocytes and neurons, which may be responsible for HIV-1 pathogenesis. Expert opinion Recent findings suggest that CYP-mediated oxidative stress is a novel pathway that may be involved in smoking-mediated HIV-1 pathogenesis, including HIV-1 replication and drug–drug interaction. Thus, CYP and CYP-associated oxidative stress pathways may be potential targets to develop novel pharmaceuticals for HIV-1-infected smokers. Since HIV-1/TB co-infections are common, future study involving interactions between antiretroviral and antituberculosis drugs that involve CYP pathways would also help treat HIV-1/TB co-infected smokers effectively.

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“…For example, CYP1A1 mRNA levels are very high in the lung cells of smokers but typically undetectable in nonsmokers (15). CYP1A1 protein is also higher in smokers compared with nonsmokers (lung median levels of 16 versus 6.5 pmol/mg microsomal protein, respectively) (16), as well as in other tissues (17,18).…”

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confidence: 99%

Human Cytochrome P450 1A1 Structure and Utility in Understanding Drug and Xenobiotic Metabolism

Walsh

Szklarz

Scott

2013

Journal of Biological Chemistry

245

243

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Background: Human cytochrome P450 1A1 (CYP1A1) activates procarcinogens, but the basis of their binding is unknown. Results: A 2.6 Å structure with the inhibitor ␣-naphthoflavone and docking simulations suggest key active site features. Conclusion: CYP1A1 has a planar active site that restricts ligand orientations.Significance: This provides a useful framework for understanding CYP1A1 interactions with a variety of substrates and inhibitors.

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Differential induction of CYP1A1 and CYP1B1 by benzo[a]pyrene in oral squamous cell carcinoma cell lines and by tobacco smoking in oral mucosa

Cited by 33 publications

References 32 publications

Gene expression profiling of cytochromes P450, ABC transporters and their principal transcription factors in the amygdala and prefrontal cortex of alcoholics, smokers and drug-free controls by qRT-PCR

Gene expression profiling of cytochromes P450, ABC transporters and their principal transcription factors in the amygdala and prefrontal cortex of alcoholics, smokers and drug-free controls by qRT-PCR

Tobacco smoking effect on HIV-1 pathogenesis: role of cytochrome P450 isozymes

Human Cytochrome P450 1A1 Structure and Utility in Understanding Drug and Xenobiotic Metabolism

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