Differential induction of C6 glioma apoptosis and autophagy by 3β-hydroxysteroid-indolamine conjugates

Panada, Jan; Klopava, Valeriya; Kulahava, Tatsiana; Koran, Siarhei; Faletrov, Yaroslav; Frolova, Nina; Fomina, Elena; Shkumatov, Vladimir

doi:10.1016/j.steroids.2023.109326

Steroids

2023

DOI: 10.1016/j.steroids.2023.109326

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Differential induction of C6 glioma apoptosis and autophagy by 3β-hydroxysteroid-indolamine conjugates

Jan Panada,

Valeriya Klopava,

Tatsiana Kulahava

et al.

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2024

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Cited by 4 publications

References 49 publications

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Isobavachalcone induces apoptosis of colorectal cancer cells mediated by interaction with survivin: Experimental and theoretical analyses

Zhang,

Yi,

Chen

2024

Arabian Journal of Chemistry

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Isobavachalcone induces apoptosis of colorectal cancer cells mediated by interaction with survivin: Experimental and theoretical analyses

Zhang,

Yi,

Chen

2024

Arabian Journal of Chemistry

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Influence of seasonality and habitat on chemical composition, cytotoxicity and antimicrobial properties of the Libidibia ferrea

Santana,

Tanajura Mendes,

de Godoi Pereira

et al. 2024

Heliyon

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Suppressing the side reactions of pregnenolone acetylation in transgenic yeast using competitive inhibition and optimal vector‒host system

Faletrov,

Frolova,

Mauersberger

et al. 2024

Vescì Akademìì navuk Belarusì. Seryâ biâlagičnyh navuk

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The rational design of the vector‒host system and the conditions for its use is the key to its most effective use as a biocatalyst for obtaining valuable products or for testing potential bioregulators. In this work, we will consider examples of such solutions for some yeasts from the literature over the past 5 years and our experience in optimizing the reactions of 17α-hydroxylation of pregnanes with recombinant strains of the yeast S. cerevisiae and Y. lipolytica. Thus, it was known that pregnenolone and its product pregnenolone and the products of its transformation by cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17) ‒ 17 hydroxypregnenolone, dehydroepiandrosterone ‒ undergo 3-O-acetylation by the Atf2p enzyme of the yeast Saccharomyces cerevisiae. We have found that adding isoamyl or amyl alcohol to the cultivation medium significantly reduced the formation of 3-O-acetylated products by both uninduced and induced cells of transgenic yeast S. cerevisiae GRF18 YEp5117α expressing P450c17 under the control of the GAL10 promoter. In this case, the Atf2p substrate competition model was applied. A more effective solution was to use a different host microorganism and an expression promoter. The analysis of the genome of the yeast Y. lipolytica using the BLAST program showed the absence of potential analogues of Atf2p, which was confirmed by the absence of pregnenolone acetylation products. Selecting the optimal host organism is an alternative to using a strain with a deleted gene.

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Differential induction of C6 glioma apoptosis and autophagy by 3β-hydroxysteroid-indolamine conjugates

Cited by 4 publications

References 49 publications

Isobavachalcone induces apoptosis of colorectal cancer cells mediated by interaction with survivin: Experimental and theoretical analyses

Isobavachalcone induces apoptosis of colorectal cancer cells mediated by interaction with survivin: Experimental and theoretical analyses

Influence of seasonality and habitat on chemical composition, cytotoxicity and antimicrobial properties of the Libidibia ferrea

Suppressing the side reactions of pregnenolone acetylation in transgenic yeast using competitive inhibition and optimal vector‒host system

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