Differential induction of apoptosis by type A and B trichothecenes in Jurkat T-lymphocytes

Nasri, Thanakarn; Bosch, Remko R.; Voorde, Sandra ten; Fink‐Gremmels, Johanna

doi:10.1016/j.tiv.2006.01.003

Cited by 47 publications

(25 citation statements)

References 45 publications

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“…Although several studies show that T-2 can cause necrosis at extremely high concentrations (475 ng/ml) (Nasri et al, 2006), our data show that the concentrations we used induced necrosis at a negligible rate (mostly in less than 2% of blastomeres).…”

Section: Acta Veterinaria Hungarica 64 2016contrasting

confidence: 77%

T-2 mycotoxin slows down the development of mouse blastocysts, decreases their blastomere number and increases chromatin damage

Somoskői

Kovács

Cseh

2016

Acta Veterinaria Hungarica

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The mycotoxin T-2 has many harmful effects on mammalian cells and reproductive functions. In the present study, the in vitro effect of T-2 toxin on mouse blastocysts was examined. Embryos were cultured in media supplemented with 0.5, 0.75 and 1 ng/ml T-2. Different exposure times were applied [96 h (treatment I) or 24 h following 72 h in toxin-free media (treatment II)]. Blastomere number, nuclear chromatin status and blastocoel formation were investigated in blastocysts. Our data show that the effect of T-2 toxin may vary depending on the stage of the embryo at the start of exposure. At 96 h of exposure, the blastocysts had blastomeres with normal chromatin quality but their developmental potential was decreased. After 24 h of exposure applied following a 72-h culture, blastomeres had a higher level of chromatin damage, although their developmental potential was the same as in the control embryos. In both cases, decreased mitotic rate was found, which resulted in decreased blastomere number even at low toxin concentration.

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Section: Acta Veterinaria Hungarica 64 2016contrasting

confidence: 77%

T-2 mycotoxin slows down the development of mouse blastocysts, decreases their blastomere number and increases chromatin damage

Somoskői

Kovács

Cseh

2016

Acta Veterinaria Hungarica

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“…Nasri et al (2006) have shown that in Jurkat T-lymphocytes NIV and DON induce citotoxicity (the IC50 value for mitochondrial activity was 0.4 µM), and the mechanism is mainly via apoptosis. In fact, they observed phosphatidylserine externalization, mitochondrial release of…”

Section: Cytotoxicitymentioning

confidence: 99%

Scientific information on mycotoxins and natural plant toxicants

Battilani

Costa

Dossena

et al. 2009

EFSA Supporting Publications

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“…Cells positively stained with Annexin V and PI (+/+) are considered to be in the late stages of apoptosis. Annexin V positive but PI negative cells (+/-) indicate cells in the early stages of apoptosis, while cells not stained with Annexin V but PI positive (-/+) are considered necrotic [22]. The method for this assay was adapted from Nasri et al, with some modification [22].…”

Section: F Flow Cytometric Analysismentioning

confidence: 99%

“…Annexin V positive but PI negative cells (+/-) indicate cells in the early stages of apoptosis, while cells not stained with Annexin V but PI positive (-/+) are considered necrotic [22]. The method for this assay was adapted from Nasri et al, with some modification [22]. Briefly, Jurkat cells at 3×10 5 cells/ml were treated with the seed extracts at 1×IC 50 and 2×IC 50 concentrations (400 µg/ml and 800 µg/ml for Rianthong; 300 µg/ml and 600 µg/ml for Jumbo; and 200 µg/ml and 400 µg/ml for Taiwan) at different incubation times (12 and …”

Section: F Flow Cytometric Analysismentioning

confidence: 99%

Apoptosis Induction Effect of Three Jujube Cultivars in HepG2 and Jurkat Cell Lines

Taechakulwanijya¹,

Weerapreeyakul²,

Barusrux³

et al. 2013

IJBBB

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Abstract-The potential cancer preventive effects of water and ethanolic extracts of three new jujube cultivars were examined in HepG2 and Jurkat cancer cell lines. Apoptosis induction was detected from alteration of nuclei morphology via DAPI staining, and apoptosis death mode was detected via Annexin V-FITC and propidium iodide fluorescence flow cytometry. Results showed that ethanolic jujube seed extracts exhibited antiproliferation only in the Jurkat cell line. Cultivar Taiwan exerted high antiproliferation effect (IC 50 =232.4±7.8 µg/ml) > Jumbo (IC 50 =312.0±18.3 µg/ml) > Rianthong (IC 50 =401.6±9.9 µg/ml), respectively (p<0.05). Melphalan (a positive control) exhibited significant antiproliferation in both HepG2 (IC 50 =37.5±3.9 µg/ml), and Jurkat cell lines (IC 50 =119.1±10.4 µg/ml) but also had cytotoxic effect on the normal Vero cell line (IC 50 =75.0±2.3 µg/ml). Cancer is the severe chronic disease that is found worldwide with increasingly high rate of morbidity and mortality [16]. Therefore, it is of interest to search for cancer preventive agent from natural source. One preferable pharmacodynamic endpoint for cancer treatment is via inducing apoptotic cell death [17]. Hence, apoptosis induction is the primary goal of chemotherapy. Apoptosis is the cell mechanism that balances between cancer cell proliferation and damage irreparable cell including damage DNA. Then, dead cells are phagocytosed by macrophages. The advantage of this death mode does not lead to inflammation in neighboring cells as same as necrosis [17].According to the reports of biological activity of jujubes and new cultivars of Thai jujubes have been cross-bred, this study was aimed to investigate the apoptosis induction effect of the jujube seed extract of three jujube cultivars (i.e., Rianthong, Jumbo and Taiwan) against the human hepatocellular carcinoma HepG2 and human leukemic Jurkat cell lines compared to the normal African green monkey kidney Vero cells. II. MATERIALS AND METHODS A. Plant Materials and ReagentsThe jujube fruits cultivars Rianthong (R), Taiwan (T) and Jumbo (J) were purchased from local markets in Khon Kaen, Thailand in 2012. The Jumbo and Taiwan cultivars are the result of a cross between Z. mauritiana and Z. jujuba, but cultivar (Rianthong) remains unidentified. The cell culture media and fetal bovine serum were purchased from GIBCO, Invitrogen Corporation (USA.). Neutral red dye was purchased from Sigma Chemicals Co. (USA), and melphalan (mel; an apoptosis induction positive control) was bought from Sigma-Aldrich Chemie GmbH (Germany). Apoptotic detection kit was bought from eBiosciences (USA). Apoptosis Induction Effect of Three Jujube Cultivars inHepG2 and Jurkat Cell Lines Natthanan Taechakulwanijya, Natthida Weerapreeyakul, Sahapat Barusrux, and Sirithorn SiriamornpunInternational Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 3, No. 6, November 2013

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Differential induction of apoptosis by type A and B trichothecenes in Jurkat T-lymphocytes

Cited by 47 publications

References 45 publications

T-2 mycotoxin slows down the development of mouse blastocysts, decreases their blastomere number and increases chromatin damage

T-2 mycotoxin slows down the development of mouse blastocysts, decreases their blastomere number and increases chromatin damage

Scientific information on mycotoxins and natural plant toxicants

Apoptosis Induction Effect of Three Jujube Cultivars in HepG2 and Jurkat Cell Lines

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