Differential induction and suppression of the potato innate immune system in response to Alfalfa mosaic virus infection

Abdelkhalek, Ahmed; Al-Askar, Abdulaziz A.; Hafez, Elsayed E.

doi:10.1016/j.pmpp.2020.101485

Cited by 24 publications

(33 citation statements)

References 63 publications

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“…These results suggested that the biologically synthesized ZnO NPs via M. spicata may mediate the activation of the induced systemic resistance (ISR) and can play a major role in tomato SAR against TMV infection. Many researchers reported that increased resistance against virulent pathogens was associated with an increased in PAL and PR-1 expression and elevated endogenous SA accumulation levels inside plant tissues [65][66][67][68][69]. The obtained results are in agreement with Cai et al [32] who reported that upon TMV infection, chemically prepared ZnO NPs increased the expression level of the tobacco PR-1 gene that resulted in the initiation of the SA signaling pathway.…”

Section: Transcriptional Levels Of Defense-related Genessupporting

confidence: 86%

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Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

2020

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Globally, plant viral infection is one of the most difficult challenges of food security, where considerable losses in crop production occur. Nanoparticles are an effective control agent against numerous plant pathogens. However, there is limited knowledge concerning their effects against viral infection. In the present study, the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous leaf extract of Mentha spicata was achieved. X-ray diffraction patterns confirmed the crystalline nature of the prepared ZnO NPs. Dynamic light scattering and scanning electron microscopy analyses revealed that the resultant ZnO NPs were spherical in shape with a particle size ranged from 11 to 88 nm. Fourier transmission infrared spectroscopy detected different functional groups, capping and stability agents, and showed Zn-O bond within wavenumber of 487 cm−1. Under greenhouse conditions, the antiviral activity of biological synthesized ZnO NPs (100 µg/mL) against Tobacco mosaic virus (TMV) was evaluated. The double foliar application of the prepared ZnO NPs, 24 h before and 24 h after TMV-inoculation, was the most effective treatment that showed a 90.21% reduction of viral accumulation level and disease severity. Additionally, the transcriptional levels of PAL, PR-1 (salicylic acid marker gene), CHS, and POD genes were induced and up-regulated in all ZnO NPs treated plants. Notably, the results exhibited that aqueous extract of Mentha spicata was an effective reducing agent for the green synthesis of ZnO NPs, which showed significant antiviral activity. Finally, the detected protective and curative activity of ZnO NPs against TMV can encourage us to recommend its application for plant viral disease management. To our knowledge, this is the first study describing the antiviral activity of the green synthesized ZnO NPs.

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Section: Transcriptional Levels Of Defense-related Genessupporting

confidence: 86%

“…Flavonoids, secondary metabolites, compounds play important roles in plant resistance against pathogenic microorganisms, including viruses [69][70][71]. Once infection occurs, flavonoids transport to the infection sites, induce hypersensitivity reactions and activate programmed cell death [72,73].…”

Section: Transcriptional Levels Of Defense-related Genesmentioning

confidence: 99%

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

2020

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“…Source of the viral isolate. Alfalfa mosaic virus Kh1 isolate (Acc# MN099289) used in this study was previously isolated from infected potato plants 52 and maintained continuously on Nicotiana glutinosa plants under greenhouse conditions. www.nature.com/scientificreports/ Source of bacterial isolate, biochemical tests characterization and culture filtrate preparation.…”

Section: Discussionmentioning

confidence: 99%

“…The down-regulation of both genes, PAL and C4H , in infected potato tissues (T2) reflects the suppressor activity of AMV. Many researchers reported that the viral infection was associated with down-regulation or decreasing in PAL and C4H activity 25 , 51 , 52 . Besides its role in plant defense, PAL involved in the biosynthesis of salicylic acid (SA) that plays an essential role in the induction of plant systemic resistance pathogens 50 , 53 – 55 .…”

Section: Discussionmentioning

confidence: 99%

“…Different primer sets specific for polyphenolic-related genes were synthesized according to previous studies ( Table 5). The housekeeping gene EF1-α (Table 5) was used as a reference gene in order to normalization of the transcript expression levels 52 . Each sample in all reactions was run in triplicate using Rotor-Gene 6000 (QIAGEN, ABI System, USA) with the SYBR Green PCR Master Mix (Fermentas, USA) and performed according to previously reported 100 .…”

Section: Greenhouse Experimental Designmentioning

confidence: 99%

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Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Abdelkhalek

Al-Askar

Behiry

2020

Sci Rep

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Alfalfa mosaic virus (AMV) is a worldwide distributed virus that has a very wide host range and causes significant crop losses of many economically important crops, including potato (Solanum tuberosum L.). In this study, the antiviral activity of Bacillus licheniformis strain POT1 against AMV on potato plants was evaluated. The dual foliar application of culture filtrate (CF), 24 h before and after AMV-inoculation, was the most effective treatment that showed 86.79% reduction of the viral accumulation level and improvement of different growth parameters. Moreover, HPLC analysis showed that a 20 polyphenolic compound was accumulated with a total amount of 7,218.86 and 1606.49 mg/kg in POT1-treated and non-treated plants, respectively. Additionally, the transcriptional analysis of thirteen genes controlling the phenylpropanoid, chlorogenic acid and flavonoid biosynthetic pathways revealed that most of the studied genes were induced after POT1 treatments. The stronger expression level of F3H, the key enzyme in flavonoid biosynthesis in plants, (588.133-fold) and AN2, anthocyanin 2 transcription factor, (97.005-fold) suggested that the accumulation flavonoid, especially anthocyanin, might play significant roles in plant defense against viral infection. Gas chromatography-mass spectrometry (GC-MS) analysis showed that pyrrolo[1,2-a]pyrazine-1,4-dione is the major compound in CF ethyl acetate extract, that is suggesting it acts as elicitor molecules for induction of systemic acquired resistance in potato plants. To our knowledge, this is the first study of biological control of AMV mediated by PGPR in potato plants.

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Foliar Application of Rhizobium leguminosarum bv. viciae Strain 33504-Borg201 Promotes Faba Bean Growth and Enhances Systemic Resistance Against Bean Yellow Mosaic Virus Infection

Yassin,

Aseel,

Khalil

et al. 2024

Curr Microbiol

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Differential induction and suppression of the potato innate immune system in response to Alfalfa mosaic virus infection

Cited by 24 publications

References 63 publications

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

Foliar Application of Rhizobium leguminosarum bv. viciae Strain 33504-Borg201 Promotes Faba Bean Growth and Enhances Systemic Resistance Against Bean Yellow Mosaic Virus Infection

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