Differential impact of heat stress on the expression of chloroplast-encoded genes

Данилова, М. Н.; Kudryakova, N. V.; Андреева, А. А.; Дорошенко, А. С.; Pojidaeva, E. S.; Kusnetsov, V. V.

doi:10.1016/j.plaphy.2018.05.023

Cited by 30 publications

(16 citation statements)

References 40 publications

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“…It is worth noting the enhanced expression of NEP genes in salt-stressed seedlings, which did not result in a significant up-regulation of plastid genes solely transcribed by NEP. This result somehow resembles the effect of heat stress on PEP and NEP expression, as reported by Danilova et al [50]. These authors proposed heat stress causing PEP deficiency, which, in turn, would result in a compensatory increase in NEP expression at the mRNA level.…”

Section: Discussionsupporting

confidence: 87%

“…GUN1 might be an important component of the NEP-dependent compensatory mechanism, because Tadini et al [63] recently found that GUN1 physically interacts with RPOTp and promotes its activity when PEP function is impaired. Our results, and those of Danilova et al [50], suggest that such a mechanism would also act in wild-type plants to help them cope with adverse environmental stimuli, like salt and heat stress. Notwithstanding, further research is required to better understand the mechanisms involved in reprogramming nuclear and plastid gene expressions in order to adapt chloroplast function and plant activity to salinity and other abiotic stresses.…”

Section: Discussionsupporting

confidence: 77%

“…Our results showed that the transcript levels of NEP genes significantly increased in response to salinity, albeit to different extents, because the mRNAs of genes RPOTmp and RPOTp respectively accumulated nearly three-and twofold more than those of RPOTm. In line with this, Danilova et al [50] reported a higher level of expression of the RPOTmp vs. RPOTp genes under heat stress in Arabidopsis. Interestingly, NEP up-regulation by salt stress was not found in genes mTERF5 or mTERF6, which suggests a differential effect of salinity on the expression of the nuclear components of the plastid gene expression machinery.…”

Section: Discussionmentioning

confidence: 59%

“…Reduced PEP activity by salt would account for the significant down-regulation of PEP-dependent PSII gene psbA, but it did not noticeably affect the expression of genes PSI psaA and psaB, also transcribed by PEP. A differential effect on the expression of plastid genes PSI and PSII has been reported in response to heat stress, which might be due to the different mechanisms governing the expression of these PS genes [50].…”

Section: Discussionmentioning

confidence: 99%

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Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance

Lidón-Soto

Núñez‐Delegido

Pastor-Martínez

et al. 2020

Plants

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Plastid gene expression (PGE) must adequately respond to changes in both development and environmental cues. The transcriptional machinery of plastids in land plants is far more complex than that of prokaryotes. Two types of DNA-dependent RNA polymerases transcribe the plastid genome: a multimeric plastid-encoded polymerase (PEP), and a monomeric nuclear-encoded polymerase (NEP). A single NEP in monocots (RPOTp, RNA polymerase of the T3/T7 phage-type) and two NEPs in dicots (plastid-targeted RPOTp, and plastid- and mitochondrial-targeted RPOTmp) have been hitherto identified. To unravel the role of PGE in plant responses to abiotic stress, we investigated if Arabidopsis RPOTp could function in plant salt tolerance. To this end, we studied the sensitivity of T-DNA mutants scabra3-2 (sca3-2) and sca3-3, defective in the RPOTp gene, to salinity, osmotic stress and the phytohormone abscisic acid (ABA) required for plants to adapt to abiotic stress. sca3 mutants were hypersensitive to NaCl, mannitol and ABA during germination and seedling establishment. Later in development, sca3 plants displayed reduced sensitivity to salt stress. A gene ontology (GO) analysis of the nuclear genes differentially expressed in the sca3-2 mutant (301) revealed that many significantly enriched GO terms were related to chloroplast function, and also to the response to several abiotic stresses. By quantitative RT-PCR (qRT-PCR), we found that genes LHCB1 (LIGHT-HARVESTING CHLOROPHYLL a/b-BINDING1) and AOX1A (ALTERNATIVE OXIDASE 1A) were respectively down- and up-regulated in the Columbia-0 (Col-0) salt-stressed plants, which suggests the activation of plastid and mitochondria-to-nucleus retrograde signaling. The transcript levels of genes RPOTp, RPOTmp and RPOTm significantly increased in these salt-stressed seedlings, but this enhanced expression did not lead to the up-regulation of the plastid genes solely transcribed by NEP. Similar to salinity, carotenoid inhibitor norflurazon (NF) also enhanced the RPOTp transcript levels in Col-0 seedlings. This shows that besides salinity, inhibition of chloroplast biogenesis also induces RPOTp expression. Unlike salt and NF, the NEP genes were significantly down-regulated in the Col-0 seedlings grown in ABA-supplemented media. Together, our findings demonstrate that RPOTp functions in abiotic stress tolerance, and RPOTp is likely regulated positively by plastid-to-nucleus retrograde signaling, which is triggered when chloroplast functionality is perturbed by environmental stresses, e.g., salinity or NF. This suggests the existence of a compensatory mechanism, elicited by impaired chloroplast function. To our knowledge, this is the first study to suggest the role of a nuclear-encoded plastid-RNA polymerase in salt stress tolerance in plants.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance

Lidón-Soto

Núñez‐Delegido

Pastor-Martínez

et al. 2020

Plants

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“…Hence, the capacity to repair photodamaged PSII strongly depends on the ability to generate new D1 protein. Chloroplasts can adjust the transcriptional efficiency of psbA during photoinhibition under adverse environmental conditions such as high light and temperature [ 66 , 67 , 68 ]. During chloroplast evolution, several nucleus-encoded proteins have developed the ability to regulate psbA transcription in order to repair photodamaged PSII under adverse environmental conditions.…”

Section: Chloroplast Gene Expression and Environmental Stressmentioning

confidence: 99%

The Role of Chloroplast Gene Expression in Plant Responses to Environmental Stress

Zhang

et al. 2020

IJMS

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Chloroplasts are plant organelles that carry out photosynthesis, produce various metabolites, and sense changes in the external environment. Given their endosymbiotic origin, chloroplasts have retained independent genomes and gene-expression machinery. Most genes from the prokaryotic ancestors of chloroplasts were transferred into the nucleus over the course of evolution. However, the importance of chloroplast gene expression in environmental stress responses have recently become more apparent. Here, we discuss the emerging roles of the distinct chloroplast gene expression processes in plant responses to environmental stresses. For example, the transcription and translation of psbA play an important role in high-light stress responses. A better understanding of the connection between chloroplast gene expression and environmental stress responses is crucial for breeding stress-tolerant crops better able to cope with the rapidly changing environment.

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A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid

Zhang,

Wang

et al. 2023

Advanced Science

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Here, a sucrose non‐fermenting‐1‐related protein kinase alpha subunit (TaSnRK1α‐1A) is identified as associated with cold stress through integration of genome‐wide association study, bulked segregant RNA sequencing, and virus‐induced gene silencing. It is confirmed that TaSnRK1α positively regulates cold tolerance by transgenes and ethyl methanesulfonate (EMS) mutants. A plastid‐lipid‐associated protein 6, chloroplastic‐like (TaPAP6L‐2B) strongly interacting with TaSnRK1α‐1A is screened. Molecular chaperone DJ‐1 family protein (TaDJ‐1‐7B) possibly bridged the interaction of TaSnRK1α‐1A and TaPAP6L‐2B. It is further revealed that TaSnRK1α‐1A phosphorylated TaPAP6L‐2B. Subsequently, a superior haplotype TaPAP6L‐2B30S/38S is identified and confirmed that both R30S and G38S are important phosphorylation sites that influence TaPAP6L‐2B in cold tolerance. Overexpression (OE) and EMS‐mutant lines verified TaPAP6L positively modulating cold tolerance. Furthermore, transcriptome sequencing revealed that TaPAP6L‐2B‐OE lines significantly increased jasmonic acid (JA) content, possibly by improving precursor α‐linolenic acid contributing to JA synthesis and by repressing JAR1 degrading JA. Exogenous JA significantly improved the cold tolerance of wheat plants. In summary, TaSnRK1α profoundly regulated cold stress, possibly through phosphorylating TaPAP6L to increase endogenous JA content of wheat plants.

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Differential impact of heat stress on the expression of chloroplast-encoded genes

Cited by 30 publications

References 40 publications

Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance

Arabidopsis Plastid-RNA Polymerase RPOTp Is Involved in Abiotic Stress Tolerance

The Role of Chloroplast Gene Expression in Plant Responses to Environmental Stress

A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid

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