Differential Immune Response of Lactobacillus plantarum 286 Against Salmonella Typhimurium Infection in Conventional and Germ-Free Mice

Santos, Tizá Teles; Ornellas, Roberta Maria Dos Santos; Acúrcio, Leonardo Borges; Sandes, S.H.C.; Costa, Andréa Miúra da; Uetanabaro, Ana Paula Trovatti; Nicoli, Jacques Robert; Vinderola, Gabriel

doi:10.1007/5584_2020_544

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Manuel, Elena, Carolina, and Gabriela (2017) showed that probiotic supplementation ( L. casei and L. paracasei ) improved the intestinal villi's length and cellularity, increasing the phagocytic activity of peritoneal and spleen macrophages and the humoral response and protecting against Salmonella infection. Santos et al (2020) found that the administration of L. plantarum reduced the tissue damage caused by S. typhimurium , and Hirano et al (2017) showed that both live and heat‐killed L. plantarum had an inhibitory effect on the gut colonization of S. typhimurium and its invasion into liver and spleen tissues. In another study, chickens challenged with S. enteritidis receiving a diet containing B. coagulans showed a higher villus–crypt ratio and increased goblet cell count (Zhen et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of oral administration of mixed probiotic spores of Bacillus subtilis and Bacillus coagulans on gut microbiota changes and intestinal and liver damage of rats infected with Salmonella Typhimurium

Mazkour

Shekarforoush

Basiri

et al. 2022

Journal of Food Safety

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Although the intestinal lumen is a significant way to enter pathogens such as Salmonella, they face a dense microbiota in the gut lumen. This community confers health benefits. Probiotics enhance the colonization resistance and direct inhibition of pathogens, which are essential in reducing the incidence and duration of gastroenteritis and histopathological complications of enteric pathogens. In this study, the spores of two probiotic bacteria, Bacillus subtilis and Bacillus coagulans, were fed to rats for 3 weeks through their daily water intake. After that, Salmonella typhimurium was gavaged to the rats. On days 1, 3, 5, and 7 after gavaging, intestinal microbiota were evaluated, and on days 3, 5, and 7 after gavaging, gut and liver histopathological changes were also evaluated. Salmonella caused no changes in the spore and aerobic bacteria population compared to the control group, but it increased anaerobic and coliform bacteria and reduced lactic acid bacteria (LAB). But B. subtilis and B. coagulans reduced anaerobic and coliform bacteria and decreased the LAB population following Salmonella infection. Also, the histopathological evaluation showed that these probiotics attenuated the shortening and fusion of villi, mononuclear cell infiltration, submucosal edema, and epithelial necrosis in the intestine, hepatocyte degeneration, and mononuclear cell infiltration in the liver, which occurred due to Salmonella infection. B. subtilis and B. coagulans help the maintenance of the gut microflora population and reduce the histopathological effects when exposed to Salmonella, which causes the reduction of these pathogen's effects and early recovery.

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Section: Discussionmentioning

confidence: 99%

Protective effects of oral administration of mixed probiotic spores of Bacillus subtilis and Bacillus coagulans on gut microbiota changes and intestinal and liver damage of rats infected with Salmonella Typhimurium

Mazkour

Shekarforoush

Basiri

et al. 2022

Journal of Food Safety

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“…S. typhimurium infection promotes the expression of pro-in ammatory cytokines [ 30] . L. plantarum exert a bene cial role in relieving in ammation caused by S. typhimurium [ 31] . For example, L. plantarum ZLP001 reduced TNF-α and IL-6 signi cantly [ 32] .…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum regulated intestinal microbial community and cytokines to inhibit Salmonella typhimurium infection

Liu

Sun

Liao

et al. 2022

Preprint

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Lactic acid bacteria (LAB) are recognized as food-grade safe microorganisms and have many beneficial effects. LAB could maintain the host intestinal homeostasis, and regulate intestinal microbial community to exert antibacterial effects. In this study, Lactobacillus plantarum (L. plantarum) strain isolated from pig intestine was orally administered to C57BL/6 mice, and mice were then infected with Salmonella typhimurium. The protective effects of L. plantarum were evaluated by monitoring body weight loss, survival rates, bacterial loads in tissue, cecal histopathology analysis, and cytokine secretion. 16S rRNA gene sequencing was also utilized to detect the dynamics of the cecal microbial community in C57BL/6 mice. We found that L. plantarum could significantly reduce the body weight loss and improve the survival rates. Counting of bacterial loads displayed that L. plantarum decreased Salmonella transplanting in spleen or liver. Intestinal histopathology showed that it alleviated the intestinal injury caused by Salmonella, inhibited the secretion of pro-inflammatory cytokines and promoted anti-inflammatory cytokines. In addition, L. plantarum also significantly ameliorated the intestinal gut microbiome disturbance caused by Salmonella and restored the balance among dominant gut microbiome. It displayed an obvious increase of beneficial bacteria including Lactobacillus and Bacteroidetes, and reduction of pathogenic bacteria like Proteobacteria. In conclusion, L. plantarum could regulate microbial community to inhibit Salmonella typhimurium infection.

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“…A particular global concern is the emerge of multidrug-resistant by different Salmonella serotypes that difficult the treatment of individuals affected with severe disease ( 38 , 39 ). Several studies have documented the protective effect of probiotics preventing Salmonella infections ( 13 – 15 , 22 , 40 ). S. Typhimurium infection was associated with disruption of tight junction and increased epithelial barrier permeability ( 41 , 42 ).…”

Section: Discussionmentioning

confidence: 99%

Probiotic Bacteria and Their Cell Walls Induce Th1-Type Immunity Against Salmonella Typhimurium Challenge

et al. 2021

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Probiotics have been associated with a variety of health benefits. They can act as adjuvant to enhance specific immune response. Bacterial cell wall (CW) molecules are key structures that interact with host receptors promoting probiotic effects. The adjuvant capacity underlying this sub-cellular fraction purified from Lactobacillus casei CRL431 and L. paracasei CNCMI-1518 remains to be characterized. We interrogated the molecular and cellular events after oral feeding with probiotic-derived CW in addition to heat-inactivated Salmonella Typhimurium and their subsequent protective capacity against S. Typhimurium challenge. Intact probiotic bacteria were orally administered for comparison. We find that previous oral feeding with probiotics or their sub-cellular fraction reduce bacterial burden in spleen and liver after Salmonella challenge. Antibody responses after pathogen challenge were negligible, characterized by not major changes in the antibody-mediated phagocytic activity, and in the levels of total and Salmonella-specific intestinal sIgA and serum IgG, respectively. Conversely, the beneficial effect of probiotic-derived CW after S. Typhimurium challenge were ascribed to a Th1-type cell-mediated immunity which was characterized by augmentation of the delayed-type hypersensitivity response. The cell-mediated immunity associated with the oral feeding with probiotic-derived CW was accompanied with a Th1-cell polarizing cytokines, distinguished by increase IFN-γ/IL-4 ratio. Similar results were observed with the intact probiotics. Our study identified molecular events associated with the oral administration of sub-cellular structures derived from probiotics and their adjuvant capacity to exert immune modulatory function.

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Differential Immune Response of Lactobacillus plantarum 286 Against Salmonella Typhimurium Infection in Conventional and Germ-Free Mice

Cited by 7 publications

References 29 publications

Protective effects of oral administration of mixed probiotic spores of Bacillus subtilis and Bacillus coagulans on gut microbiota changes and intestinal and liver damage of rats infected with Salmonella Typhimurium

Protective effects of oral administration of mixed probiotic spores of Bacillus subtilis and Bacillus coagulans on gut microbiota changes and intestinal and liver damage of rats infected with Salmonella Typhimurium

Lactobacillus plantarum regulated intestinal microbial community and cytokines to inhibit Salmonella typhimurium infection

Probiotic Bacteria and Their Cell Walls Induce Th1-Type Immunity Against Salmonella Typhimurium Challenge

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