Differential
            <i>Listeria monocytogenes</i>
            Strain Survival and Growth in Katiki, a Traditional Greek Soft Cheese, at Different Storage Temperatures

Kagkli, Dafni Maria; Iliopoulos, Vassilios; Stergiou, Virginia; Lazaridou, Anna; Nychas, George‐John E.

doi:10.1128/aem.01571-08

Cited by 39 publications

(26 citation statements)

References 27 publications

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“…This was something quite expected, given the well-known greater capability of L. monocytogenes to adapt to survive and grow under a wider range of environmental conditions than S. enterica (18). In order to discriminate between the three different L. monocytogenes strains with regard to their ability to contribute to the formation and maintenance of the mixed culture biofilm communities (mono-/dual species) as well as to their antimicrobial recalcitrance, a promising PFGE approach, already used by our team (15,34), was followed. In this study, PFGE analysis revealed interesting information regarding strain occurrence following the different treatments (control, BC, NaClO, PA, and HP-PA) (Fig.…”

Section: Discussionmentioning

confidence: 99%

Differential Biofilm Formation and Chemical Disinfection Resistance of Sessile Cells of Listeria monocytogenes Strains under Monospecies and Dual-Species (with Salmonella enterica) Conditions

Kostaki

Chorianopoulos

Braxou

et al. 2012

Appl Environ Microbiol

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145

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ABSTRACTThis study aimed to investigate the possible influence of bacterial intra- and interspecies interactions on the ability ofListeria monocytogenesandSalmonella entericato develop mixed-culture biofilms on an abiotic substratum, as well as on the subsequent resistance of sessile cells to chemical disinfection. Initially, three strains from each species were selected and left to attach and form biofilms on stainless steel (SS) coupons incubated at 15°C for 144 h, in periodically renewable tryptone soy broth (TSB), under either monoculture or mixed-culture (mono-/dual-species) conditions. Following biofilm formation, mixed-culture sessile communities were subjected to 6-min disinfection treatments with (i) benzalkonium chloride (50 ppm), (ii) sodium hypochlorite (10 ppm), (iii) peracetic acid (10 ppm), and (iv) a mixture of hydrogen peroxide (5 ppm) and peracetic acid (5 ppm). Results revealed that both species reached similar biofilm counts (ca. 105CFU cm−2) and that, in general, interspecies interactions did not have any significant effect either on the biofilm-forming ability (as this was assessed by agar plating enumeration of the mechanically detached biofilm bacteria) or on the antimicrobial resistance of each individual species. Interestingly, pulsed-field gel electrophoresis (PFGE) analysis clearly showed that the threeL. monocytogenesstrains did not contribute at the same level either to the formation of mixed-culture sessile communities (mono-/dual species) or to their antimicrobial recalcitrance. Additionally, the simultaneous existence inside the biofilm structure ofS. entericacells seemed to influence the occurrence and resistance pattern ofL. monocytogenesstrains. In sum, this study highlights the impact of microbial interactions taking place inside a mixed-culture sessile community on both its population dynamics and disinfection resistance.

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Section: Discussionmentioning

confidence: 99%

Differential Biofilm Formation and Chemical Disinfection Resistance of Sessile Cells of Listeria monocytogenes Strains under Monospecies and Dual-Species (with Salmonella enterica) Conditions

Kostaki

Chorianopoulos

Braxou

et al. 2012

Appl Environ Microbiol

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145

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“…La listeriosis no invasiva se manifiesta por síntomas de gastroenteritis (1,5,28,43), como diarrea, fiebre, cefalea y mialgias, y su periodo de incubación es corto (1,4,13,15,19,21,28,(43)(44)(45) Los alimentos considerados de alto riesgo para contraer listeriosis son los listos para el consumo (1,5,(9)(10)(11)16,17,28,(30)(31)(32)34,47,50,(63)(64)(65)(66). Ésta es una categoría amplia y heterogénea de alimentos (incluye bebidas) que no van a tener ningún proceso de cocción antes de su consumo, o que aparentemente son aptos para el consumo sin cocción (5,16), y varían de unos países a otros en relación con los hábitos alimentarios, la disponibilidad de la cadena de frío y la temperatura máxima en el punto de venta (1,5).…”

Section: Los Centers For Disease Control and Preventionunclassified

“…Con la clasificación alimentaria expuesta anteriormente, el criterio establecido en los Estados Unidos para aceptar como inocuos los alimentos listos para el consumo en los que puede crecer L. monocytogenes, es de cero tolerancia (10,16,47,48,65,68,69). Europa acepta niveles hasta de 100 unidades formadoras de colonias (UFC) para algunos alimentos que no estén destinados a poblaciones vulnerables (47).…”

Section: Los Centers For Disease Control and Preventionunclassified

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Presencia de Listeria monocytogenes en alimentos listos para el consumo, procedentes de plazas de mercado y delicatessen de supermercados de cadena, Bogotá, D.C, 2002-2008

Munoz¹,

Vargas

Otero

et al. 2011

biomedica

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Introducción. El aumento de las enfermedades transmitidas por alimentos se ha constituido en un problema de salud pública. La listeriosis, cuyo agente etiológico es Listeria monocytogenes, es considerada de gran relevancia entre las ellas y los alimentos listos para el consumo de ventas al por menor son de gran riesgo. Objetivo. Identificar la presencia de L. monocytogenes en alimentos listos para el consumo, procedentes de plazas de mercado y delicatessen de Bogotá. Materiales y métodos. Se llevó a cabo un estudio descriptivo transversal con componente analítico, en el cual se analizaron 600 alimentos, 300 de delicatessen y 300 de plazas de mercado. Se utilizaron metodologías de referencia de presencia o ausencia de L. monocytogenes en 25 g o mililitros de alimento. Resultados. De las 600 muestras analizadas, 68 fueron positivas para L. monocytogenes (11,3 %), 26 (38,25 %) procedieron de delicatessen, 42 (61,76 %) de plazas de mercado. El serotipo aislado con mayor frecuencia fue 4b en 53 (78 %) aislamientos. Los quesos frescos y los quesos madurados mostraron mayor contaminación de L. monocytogenes que el resto de alimentos del estudio (p=0,0009/0,0003). Conclusión. Los resultados indican que estos alimentos son vehículos de transmisión del microorganismo, convirtiéndolos en potenciales alimentos de alto riesgo; deben ser vigilados y controlados por la autoridad competente. Se requieren programas para implementar la normativa sobre vigilancia, reducción y control de este microorganismo con miras hacia la prevención de las enfermedades transmitidas por alimentos.Palabras clave: Listeria monocytogenes, enfermedades transmitidas por alimentos, listeriosis, salud pública. Presence of Listeria monocytogenes in ready-to-eat foods available in open markets, delicatessens and supermarkets, Bogotá, 2002-2008Introduction. The increase in diseases transmitted by foods has become a public health problem. Listeria monocytogenes, the etiological agent of listeriosis or typical food poisoning, is considered to be among the most important food borne illnesses. High risk foods for L.monocytogenes have been recognized as foods already prepared for consumption and made available in retail outlets. Objective. The presence of L. monocytogenes was identified in prepared foods found in open markets and delicatessens in the city of Bogotá. Material and methods. A transverse descriptive study over a 7-year period, with an analytic component, in which 600 foods were analyzed--300 from delicatessens and 300 from open market places. Reference methods were used to determine the presence or absence of L. monocytogenes in 25 grams or milliliters a food sample. Results. Of the 600 samples, 68 were positive for L. monocytogenes (11.3%). Twenty-six (38.2%) were taken from delicatessens and 42 (61.8%) from open market places. The serotype most frequently isolated was 4b, with 53 (78.0%) isolations. Fresh cheeses and matured cheeses showed greater contamination with L. monocytogenes than the other foods (p<0.001 in both cases). Conclusions.The r...

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“…In severe cases, this pathogen has been shown to enter the central nervous system (CNS). Usually, Listeria monocytogenes is ingested from contaminated food, particularly seafood, soft cheeses, and meat, being that this organism grows well at a wide range of temperatures [3]. From a cellular perspective, this pathogen has developed an array of molecular mechanisms by which it is able to invade a host cell, thrive and replicate before migrating to subsequent cells all while evading the immune system of the host organism [4].…”

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confidence: 99%

Listeria monocytogenes Infects the Zebrafish Central Nervous System; A Useful Model to Analyze the Neuroimmune Response

et al. 2014

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Listeria monocytogenes is a facultative, intracellular, gram-positive bacterium that causes listeriosis in immunocompromised patients, causing death in 20%-30% of infected individuals [1,2]. In severe cases, this pathogen has been shown to enter the central nervous system (CNS). Usually, Listeria monocytogenes is ingested from contaminated food, particularly seafood, soft cheeses, and meat, being that this organism grows well at a wide range of temperatures [3]. From a cellular perspective, this pathogen has developed an array of molecular mechanisms by which it is able to invade a host cell, thrive and replicate before migrating to subsequent cells all while evading the immune system of the host organism [4]. In short, the infection begins when the bacterium is phagocytized by macrophages, where rather than being destroyed, using listeriolysin O, it escapes the m vesicle and survives in the host macrophage. From here, the bacterium proliferates and spreads to other cells in the organism.Our work set out to investigate if zebrafish could be used as a model organism to study pathogenesis of Listeria monocytogenes specifically in the CNS. Zebrafish have become more widely accepted as models in which to study bacterial pathogenesis [5]. There have been few studies which have investigated Listeria pathogenesis both in larval zebrafish [6] and in adult zebrafish [7]. Both studies have shown that zebrafish are, in general, more resistant to L. monocytogenes infection than other mammalian models used. However, neither study investigated the ability of the organism to infect the zebrafish CNS. The goal was to establish and characterize a model where the CNS of adult zebrafish was infected with Listeria monocytogenes. To accomplish this, L. monocytogenes was injected into the vitreous humor of the zebrafish eye. The fish were sacrificed on a time course and their brain was removed and prepared for either microscopy, bacterial enumeration or molecular detection. We hypothesized that the Listeria monocytogenes could enter the cells of the retina and move into the CNS by way of the optic nerve.Approximately 50% of the injected fish survived 24 hours. This increased to around 75% in later experiments, suggesting that there is a learning curve for the investigator performing the experiment. At 24, 48, 96 hours and 7 days after the injection, fish were sacrificed under anesthesia and their brains were plated on Oxford agar. Initially, fish that died prior to the first 24 hours were not positive for the presence of L. monocytogenes. Brains of fish sacrificed later than 24 hours were all positive for L. monocytogenes.At the light microscopic level, cells of the periventricular grey zone (PVGZ) were seen to be abnormally shaped and unhealthy in appearance. Transmission electron microscopy revealed the presence of Listeria in the optic tectum of the zebrafish brain. Specifically residing in and among the cells of the PVGZ. This region of the brain is the direct input of the optic nerve and the major visual processing cortex of the...

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Differential Listeria monocytogenes Strain Survival and Growth in Katiki, a Traditional Greek Soft Cheese, at Different Storage Temperatures

Cited by 39 publications

References 27 publications

Differential Biofilm Formation and Chemical Disinfection Resistance of Sessile Cells of Listeria monocytogenes Strains under Monospecies and Dual-Species (with Salmonella enterica) Conditions

Differential Biofilm Formation and Chemical Disinfection Resistance of Sessile Cells of Listeria monocytogenes Strains under Monospecies and Dual-Species (with Salmonella enterica) Conditions

Presencia de Listeria monocytogenes en alimentos listos para el consumo, procedentes de plazas de mercado y delicatessen de supermercados de cadena, Bogotá, D.C, 2002-2008

Listeria monocytogenes Infects the Zebrafish Central Nervous System; A Useful Model to Analyze the Neuroimmune Response

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