Differential heat-shock protein synthesis and response to stress in three avirulent and virulent listeria species

Morange, M.; Hévin, Brigitte; Fauve, Robert M.

doi:10.1016/s0923-2494(93)80050-9

Cited by 9 publications

(8 citation statements)

References 35 publications

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“…Proteome analysis of heat stress responses in L. monocytogenes cells revealed increased production of GroEL and DnaK, which is consistent with the role of these highly conserved Hsps, in removal of altered proteins in L. monocytogenes cells exposed to heat stress (Sokolovic et al, 1990;Morange et al, 1993). Another heat stress response proteome study identified the general stress response protein Ferritin (also referred as ferritin like proteins, Flp) to be the predominant heat stress-induced protein in this bacterium (Hebraud and Guzzo, 2000).…”

Section: Sighsupporting

confidence: 67%

The Contribution of Transcriptomic and Proteomic Analysis in Elucidating Stress Adaptation Responses ofListeria monocytogenes

Soni

Nannapaneni

Tasara

2011

Foodborne Pathogens and Disease

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The foodborne transmission of Listeria monocytogenes requires physiological adaptation to various conditions, including the cold, osmotic, heat, acid, alkaline, and oxidative stresses, associated with food hygiene, processing, and preservation measures. We review the current knowledge on the molecular stress adaptation responses in L. monocytogenes cells as revealed through transcriptome, proteome, genetic, and physiological analysis. The adaptation of L. monocytogenes to stress exposure is achieved through global expression changes in a large number of cellular components. In addition, the cross-protection of L. monocytogenes exposed to different stress environments might be conferred through various cellular machineries that seem to be commonly activated by the different stresses. To assist in designing L. monocytogenes mitigation strategies for ready-to-eat food products, further experiments are warranted to specifically evaluate the effects of food composition, additives, preservatives, and processing technologies on the modulation of L. monocytogenes cellular components in response to specific stresses.

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Section: Sighsupporting

confidence: 67%

The Contribution of Transcriptomic and Proteomic Analysis in Elucidating Stress Adaptation Responses ofListeria monocytogenes

Soni

Nannapaneni

Tasara

2011

Foodborne Pathogens and Disease

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“…Morange et al [27] and Kushwaha and Muriana [19] further reported that the virulence (i.e., invasiveness) of L. monocytogenes was dependent upon incubation temperature and the strong adherence phenotype in L. monocytogenes , respectively, and possibly suggesting a correlation between virulence and adherence factors. In regards to food processing, higher temperatures resulting in greater levels of adherence could correlate to a greater degree of equipment surface contamination and food product contamination.…”

Section: Resultsmentioning

confidence: 99%

RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

Tiong

Muriana

2016

Pathogens

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L. monocytogenes adherence to food-associated abiotic surfaces and the development of biofilms as one of the underlying reasons for the contamination of ready-to-eat products is well known. The over-expression of internalins that improves adherence has been noted in cells growing as attached cells or at elevated incubation temperatures. However, the role of other internalin-independent surface proteins as adhesins has been uncharacterized to date. Using two strains each of weakly- and strongly-adherent L. monocytogenes as platforms for temperature-dependent adherence assays and targeted mRNA analyses, these observations (i.e., sessile- and/or temperature-dependent gene expression) were further investigated. Microplate fluorescence assays of both surface-adherent strains exhibited significant (P < 0.05) adherence at higher incubation temperature (42 °C). Of the 15 genes selected for RT-qPCR, at least ten gene transcripts recovered from cells (weakly-adherent strain CW35, strongly-adherent strain 99-38) subject to various growth conditions were over expressed [planktonic/30 °C (10), sessile/30 °C (12), planktonic/42 °C (10)] compared to their internal control (16SrRNA transcripts). Of four genes overexpressed in all three conditions tested, three and one were implicated as virulence factors and unknown function, respectively. PCR analysis of six unexpressed genes revealed that CW35 possessed an altered genome. The results suggest the presence of other internalin-independent adhesins (induced by growth temperature and/or substratum) and that a group of suspect protein members are worthy of further analysis for their potential role as surface adhesins. Analysis of the molecular basis of adherence properties of isolates of L. monocytogenes from food-associated facilities may help identify sanitation regimens to prevent cell attachment and biofilm formation on abiotic surfaces that could play a role in reducing foodborne illness resulting from Listeria biofilms.

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“…Limited information is available in the published literature on antibodies used against Listeria chaperones (17,31). We successfully detected L. monocytogenes GroEL, GroES, and DnaJ with commercial antibodies developed for the corresponding Escherichia coli chaperones.…”

Section: Discussionmentioning

confidence: 99%

Pulsed Electric Field Alters Molecular Chaperone Expression and Sensitizes Listeria monocytogenes to Heat

Lado

Bomser

Dunne

et al. 2004

Appl Environ Microbiol

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Pulsed electric field (PEF)-resistant and PEF-sensitive Listeria monocytogenes strains were sublethally treated with electric pulses at 15 kV/cm for 29 s and held at 25°C for 5 to 30 min prior to protein extraction. The levels of the molecular chaperones GroEL, GroES, and DnaJ were determined by immunoblotting. After 10 to 20 min after sublethal PEF treatment, a transient decrease in molecular chaperone expression was observed in the PEF-sensitive strain (Scott A). The levels of GroEL and DnaJ increased back to the basal expression level within 30 min. A substantial decrease in GroES expression persisted for at least 30 min after PEF treatment. Chaperone expression was suppressed after PEF treatment to a smaller extent in the PEFresistant (OSY-8578) than in the PEF-sensitive strain, and no clear expression pattern was identified in OSY-8578. Inactivation of Scott A and OSY-8578 in phosphate buffer was compared when lethal PEF (27.5 kV/cm, 144 s) and heat (55°C, 10 min) were applied in sequence. When PEF and heat treatments were applied separately, the populations of L. monocytogenes Scott A and OSY-8578 decreased 0.5 to 0.6 log CFU/ml. Cells treated first with PEF and incubated at 25°C for 10 min showed substantial sensitivity to subsequent heat treatment; the decrease in counts for Scott A and OSY-8578 was 6.1 and 2.8 log CFU/ml, respectively. The sequence and time lapse between the two treatments were crucial for achieving high inactivation rates. It is concluded that PEF sensitized L. monocytogenes to heat and that maximum heat sensitization occurred when chaperone expression was at a minimum level.

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Differential heat-shock protein synthesis and response to stress in three avirulent and virulent listeria species

Cited by 9 publications

References 35 publications

The Contribution of Transcriptomic and Proteomic Analysis in Elucidating Stress Adaptation Responses ofListeria monocytogenes

The Contribution of Transcriptomic and Proteomic Analysis in Elucidating Stress Adaptation Responses ofListeria monocytogenes

RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

Pulsed Electric Field Alters Molecular Chaperone Expression and Sensitizes Listeria monocytogenes to Heat

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