Differential growth on sutures of tendon cells derived from torn human rotator cuff

Hakimi, Osnat; Chaudhury, Salma; Murphy, Richard; Carr, Andrew

doi:10.1002/jbm.b.31993

Cited by 8 publications

(14 citation statements)

References 42 publications

(47 reference statements)

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“…Minimal tissue reaction and good ability to contain cells and fluids were the preliminary requisites when selecting a material for the creation of the patch. In a previous study, we have highlighted polydioxanone (PDO) as a candidate material for tendon repair because it was particularly compatible with tendon-derived cells in vitro (Hakimi et al, 2012). Moreover, it has an excellent history as an implant material even at large quantities of material, with acceptable host response and no severe inflammation (Curtis et al, 2005).…”

Section: Biocompatibility and Cell Attachment And Migrationmentioning

confidence: 99%

“…Moreover, it has an excellent history as an implant material even at large quantities of material, with acceptable host response and no severe inflammation (Curtis et al, 2005). However, despite excellent biocompatibility, PDO sutures (PDSII) displayed relatively low cell attachment in vitro (Hakimi et al, 2012). One challenge was therefore to design a PDO-based scaffold that will retain its biocompatibility but encourage cell attachment.…”

Section: Biocompatibility and Cell Attachment And Migrationmentioning

confidence: 99%

See 1 more Smart Citation

An electrospun polydioxanone patch for the localisation of biological therapies during tendon repair

Hakimi

Murphy²,

Stachewicz³

et al. 2012

eCM

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Rotator cuff tendon pathology is thought to account for 30-70 % of all shoulder pain. For cases that have failed conservative treatment, surgical re-attachment of the tendon to the bone with a non-absorbable suture is a common option. However, the failure rate of these repairs is high, estimated at up to 75 %. Studies have shown that in late disease stages the tendon itself is extremely degenerate, with reduced cell numbers and poor matrix organisation. Thus, it has been suggested that adding biological factors such as platelet rich plasma (PRP) and mesenchymal stem cells could improve healing. However, the articular capsule of the glenohumeral joint and the subacromial bursa are large spaces, and injecting beneficial factors into these sites does not ensure localisation to the area of tendon damage. Thus, the aim of this study was to develop a biocompatible patch for improving the healing rates of rotator cuff repairs. The patch will create a confinement around the repair area and will be used to guide injections to the vicinity of the surgical repair. Here, we characterised and tested a preliminary prototype of the patch utilising in vitro tools and primary tendon-derived cells, showing exceptional biocompatibility despite rapid degradation, improved cell attachment and that cells could migrate across the patch towards a chemoattractant. Finally, we showed the feasibility of detecting the patch using ultrasound and injecting liquid into the confinement ex vivo. There is a potential for using this scaffold in the surgical repair of interfaces such as the tendon insertion in the rotator cuff, in conjunction with beneficial factors.

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Section: Biocompatibility and Cell Attachment And Migrationmentioning

confidence: 99%

Section: Biocompatibility and Cell Attachment And Migrationmentioning

confidence: 99%

An electrospun polydioxanone patch for the localisation of biological therapies during tendon repair

Hakimi

Murphy²,

Stachewicz³

et al. 2012

eCM

View full text Add to dashboard Cite

show abstract

“…In addition, the probable negative effects of polyglactin 910 degradation products on regenerating tissues were demonstrated [10], with stubborn infl ammatory reaction [11].…”

Section: Resultsmentioning

confidence: 98%

Local Tissue Reaction to Implantation of Biodegradable Suture Materials

et al. 2014

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Morphological changes in tissues adjacent to the implant were studied 1, 2, 6, and 12 months after implantation of biodegradable suture materials (catgut thread, DemeTECH polyfilament thread, and Surgilactin monofilament thread) into subcutaneous fat of rats. Tissue reaction to implantation of different suture materials developed as usual in response to a wound process and to a foreign body. By the end of month 1. the stage of traumatic perifocal inflammation was replaced in all groups by the proliferative phase with formation of new vessels and connective tissue. A trend to reduction of the productive inflammation activity from month 1 to month 12 was noted. Complete degeneration of the material after 12 months with complete recovery of the histoarchitecture of the adjacent tissues (restitution) was detected only after implantation of DemeTECH polyfilament thread.

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“…) and PDO (Hakimi et al . ,b) have exhibited excellent cellular response and biocompatibility. Further, in vitro evaluations have also demonstrated that electrospun materials are likely to cause a lower immune response in vivo compared to the same materials processed into plain sheets (Smith et al .…”

Section: New Generation Of Rotator Cuff Patches: Synthetic Degradablmentioning

confidence: 99%

“…High concentration of lactic and glycolic acid have been found to be toxic to both tendon‐derived cells and osteoblasts, and non‐toxic concentrations decreased cell proliferation and increased differentiation in osteoblasts (Hakimi et al . ,b; Meyer et al . ).…”

Section: New Generation Of Rotator Cuff Patches: Synthetic Degradablmentioning

confidence: 99%

Synthetic and degradable patches: an emerging solution for rotator cuff repair

Hakimi

Mouthuy

Carr

2013

Int J Experimental Path

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SUMMARYThe use of rotator cuff augmentation has increased dramatically over the last 10 years in response to the high rate of failure observed after non-augmented surgery. However, although augmentations have been shown to reduce shoulder pain, there is no consensus or clear guideline as to what is the safest or most efficacious material. Current augmentations, either available commercially or in development, can be classified into three categories: non-degradable structures, extra cellular matrix (ECM)-based patches and degradable synthetic scaffolds. Non-degradable structures have excellent mechanical properties, but can cause problems of infection and loss of integrity in the long-term. ECM-based patches usually demonstrate excellent biological properties in vitro, but studies have highlighted complications in vivo due to poor mechanical support and to infection or inflammation. Degradable synthetic scaffolds represent the new generation of implants. It is proposed that a combination of good mechanical properties, active promotion of biological healing, low infection risk and bio-absorption are the ideal characteristics of an augmentation material. Among the materials with these features, those processed by electrospinning have shown great promis. However, their clinical effectiveness has yet to be proven and well conducted clinical trials are urgently required.

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Differential growth on sutures of tendon cells derived from torn human rotator cuff

Cited by 8 publications

References 42 publications

An electrospun polydioxanone patch for the localisation of biological therapies during tendon repair

An electrospun polydioxanone patch for the localisation of biological therapies during tendon repair

Local Tissue Reaction to Implantation of Biodegradable Suture Materials

Synthetic and degradable patches: an emerging solution for rotator cuff repair

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