Differential gonadotropin releasing hormone (GnRH) expression, autoregulation and effects in two models of rat luteinized ovarian cells

Sorianello, Eleonora; Fernández, Marina Olga; Catalano, Paolo N.; Mongiat, Lucas A.; Somoza, Gustavo M.; Libertun, Carlos; Lux‐Lantos, Victoria

doi:10.1016/j.lfs.2005.03.018

Cited by 6 publications

(5 citation statements)

References 52 publications

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“…Briefly, tissues were homogenized in 200 μl ice-cold 0.1 N HCl, the homogenate was centrifuged at 13,000 × g at 4 C for 30 min, and the supernatant was recovered. Samples were stored at −20 C until assayed for GnRH by RIA [40]. GnRH assay sensitivity was 1.5 pg.…”

Section: Methodsmentioning

confidence: 99%

Lack of Functional GABAB Receptors Alters Kiss1, Gnrh1 and Gad1 mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4

Giorgio

Catalano²,

López³

et al. 2013

Neuroendocrinology

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Background/Aims: Adult mice lacking functional GABA_B receptors (GABA_B1KO) show altered Gnrh1 and Gad1 expressions in the preoptic area-anterior hypothalamus (POA-AH) and females display disruption of cyclicity and fertility. Here we addressed whether sexual differentiation of the brain and the proper wiring of the GnRH and kisspeptin systems were already disturbed in postnatal day 4 (PND4) GABA_B1KO mice. Methods: PND4 wild-type (WT) and GABA_B1KO mice of both sexes were sacrificed; tissues were collected to determine mRNA expression (qPCR), amino acids (HPLC), and hormones (RIA and/or IHC). Results: GnRH neuron number (IHC) did not differ among groups in olfactory bulbs or OVLT-POA. Gnrh1 mRNA (qPCR) in POA-AH was similar among groups. Gnrh1 mRNA in medial basal hypothalamus (MBH) was similar in WTs but was increased in GABA_B1KO females compared to GABA_B1KO males. Hypothalamic GnRH (RIA) was sexually different in WTs (males > females), but this sex difference was lost in GABA_B1KOs; the same pattern was observed when analyzing only the MBH, but not in the POA-AH. Arcuate nucleus Kiss1 mRNA (micropunch-qPCR) was higher in WT females than in WT males and GABA_B1KO females. Gad1 mRNA in MBH was increased in GABA_B1KO females compared to GABA_B1KO males. Serum LH and gonadal estradiol content were also increased in GABA_B1KOs. Conclusion: We demonstrate that GABA_BRs participate in the sexual differentiation of the ARC/MBH, because sex differences in several reproductive genes, such as Gad1, Kiss1 and Gnrh1, are critically disturbed in GABA_B1KO mice at PND4, probably altering the organization and development of neural circuits governing the reproductive axis.

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Section: Methodsmentioning

confidence: 99%

Lack of Functional GABAB Receptors Alters Kiss1, Gnrh1 and Gad1 mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4

Giorgio

Catalano²,

López³

et al. 2013

Neuroendocrinology

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“…The homogenate was centrifuged for 30 min at 13,000 g at 4°C and the supernatant was recovered; the procedure was repeated. Samples were stored at Ϫ20°C until assayed for GnRH by RIA, as previously described (87). GnRH assay sensitivity was 1.5 pg, and intra-and interassay coefficients of variation were 7.1 and 11.6%, respectively.…”

Section: Gnrh Content Determinationmentioning

confidence: 99%

“…Briefly, whole hypothalami were incubated in 1.5-ml microfuge tubes containing 250 l of Krebs-Ringer bicarbonate buffer with 4.5 mg/ml glucose and 16 mM HEPES at 37°C for 6 h. After 30 min of preincubation, the medium from each tube was collected at 8.5-min intervals and replaced with fresh medium. GnRH in the incubation media was measured by RIA as previously described (87). The number of animals per group was 6 -7.…”

Section: Gnrh Pulsatility Studiesmentioning

confidence: 99%

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Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Catalano

Giorgio

Bonaventura

et al. 2010

American Journal of Physiology-Endocrinology and Metabolism

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GABA, the main inhibitory neurotransmitter, acts through GABAA/C and GABAB receptors (GABABRs); it is critical for gonadotropin regulation. We studied whether the lack of functional GABABRs in GABAB1 knockout (GABAB1KO) mice affected the gonadotropin axis physiology. Adult male and female GABAB1KO and wild-type (WT) mice were killed to collect blood and tissue samples. Gonadotropin-releasing hormone (GnRH) content in whole hypothalami (HT), olfactory bulbs (OB), and frontoparietal cortexes (CT) were determined (RIA). GnRH expression by quantitative real-time PCR (qRT-PCR) was evaluated in preoptic area-anterior hypothalamus (POA-AH), medial basal-posterior hypothalamus (MBH-PH), OB, and CT. Pulsatile GnRH secretion from hypothalamic explants was measured by RIA. GABA, glutamate, and taurine contents in HT and CT were determined by HPLC. Glutamic acid decarboxylase-67 (GAD-67) mRNA was measured by qRT-PCR in POA-AH, MBH-PH, and CT. Gonadotropin content, serum levels, and secretion from adenohypophyseal cell cultures (ACC) were measured by RIA. GnRH mRNA expression was increased in POA-AH of WT males compared with females; this pattern of expression was inversed in GABAB1KO mice. MBH-PH, OB, and CT did not follow this pattern. In GABAB1KO females, GnRH pulse frequency was increased and GABA and glutamate contents were augmented. POA-AH GAD-67 mRNA showed the same expression pattern as GnRH mRNA in this area. Gonadotropin pituitary contents and serum levels showed no differences between genotypes. Increased basal LH secretion and decreased GnRH-stimulated gonadotropin response were observed in GABAB1KO female ACCs. These results support the hypothesis that the absence of functional GABABRs alters GnRH physiology and critically affects sexual dimorphic expression of GnRH and GAD-67 in POA-AH.

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“…Luteoma genellikle gebelikle ilişkilidir ve doğumdan sonra geriler 7 . Gebelik luteoması gebelik sırasında bir tümör gibi davranabilen ovaryum genişlemesi ile karakterize nonneoplastik bir lezyondur ve gebelik sırasında koriyonik gonodotropin etkisi altında lüteinleşen stromal hücrelerin normal ovaryum parankimi ile yer değiştirmesinden veya yüksek HCG düzeyine yanıt olarak ovaryumun stromasından ya da internal teka hücelerinden gelişir 1,10 . Bu olayda hayvanın gebe kaldığına ve doğum yaptığına dair herhangi bir klinik ve makroskobik bulguya rastlanamadı.…”

Section: Discussionunclassified

Bir Köpekte Tek Taraflı Luteoma Olgusu

Yılmaz

Özyiğit

Akkoç

2017

Uludağ Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu olguda, Terrier ırkı, dişi, 13 yaşlı köpeğin sol ovaryumunda nadir olarak şekillenen luteomanın makroskobik ve mikroskobik özellikleri tanımlanmıştır. Klinik olarak bir haftadan beri süren iştahsızlık ve kusma bulguları ile Veteriner Fakültesi Kliniklerine getirilen hayvanın ultrasonografik muayenesinde batın içerisinde 13x11 cm boyutlarında tamamen hipoekoik ve yer yer normal dokulara göre farklı ekojenitede homojen yapı gösteren kitleye rastlandı. Yapılan operasyonda tümöral kitle ile beraber ovaryum ve uterus, makroskobik ve histopatolojik inceleme için patoloji laboratuvarına gönderildi. Makroskobik muayenesinde kitlenin 13x 11x 8 cm boyutlarında, orta sert kıvamlı, kesit yüzü krem renkte ve yer yer kanama alanları içerdiği görüldü. Histopatolojik incelemede, sol ovaryumun tamamen tümöral doku ile kaplanmış olduğu ve normal ovaryum yapısına ait hiçbir histolojik doku içermediği dikkati çekti. Tümörün hücre sınırları belirgin, eozinofilik sitoplazmalı, sitop-lazmasında yağ vakuolleri olan, açık veziküler çekirdekli, belirgin çekirdekçiğe sahip, yer yer ince bağ doku kordonları ile birbirinden ayrılan poligonal hücre kümelerinden oluştuğu dikkati çekti. Tümöral dokuda yer yer mitotik figürlere rastlandı. Bazı alanlarda nekroz ve kanama görüldü. Bu olayda, makroskobik ve histopatolojik inceleme sonucunda sol ovaryumda nadir görülen luteoma olgusu tanımlanmıştır.Abstract: In this case, ovarial luteoma in the left ovary in a 13-year-old Terrier was defined both macroscopically and microscopically. The dog had a history of vomiting and anorexia for a week. In palpation, sensitivity observed on the left abdominal region of the animal. Ultrasonographically a hypoechoic, round mass with homogeny architecture, 13 x 11 cm in size, located in the left abdominal region was found. The both ovary with tumoural mass and uterus were taken by operation and masses were referred to pathology laboratory for histopathological examination. Macroscopically, the left ovarial mass, 13 x 11 x 8 cm in size, was moderately firm, round, multilobulated and encapsulated and the cut surface had a yellowish appearance. There were focal areas of hemorrhages and necrosis. In microscopical examination, neoplastic cells were arranged in irregular shaped solid sheet. The nuclei were small, round to oval and had coarsely stippled chromatin. The amount of cytoplasm varied from scant to moderate and was faintly eosinophilic and finely granular or vacuolated. Luteinized cells had prominent eosinophilic cytoplasm and pale staining nuclei. Few mitotic figures were seen in the sections. The left ovary was completely invaded by neoplastic cells and the normal histological architecture was not observed. However in the right ovary and uterus was not found pathological lesion by histological examination. In the light of the macroscopical and microscopical findings the tumour was found compatible with ovarial luteo-ma.

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Differential gonadotropin releasing hormone (GnRH) expression, autoregulation and effects in two models of rat luteinized ovarian cells

Cited by 6 publications

References 52 publications

Lack of Functional GABA<sub>B</sub> Receptors Alters <b><i>Kiss1</i></b>, <b><i>Gnrh1 </i></b>and <b><i>Gad1</i></b> mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4

Lack of Functional GABA<sub>B</sub> Receptors Alters <b><i>Kiss1</i></b>, <b><i>Gnrh1 </i></b>and <b><i>Gad1</i></b> mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4

Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Bir Köpekte Tek Taraflı Luteoma Olgusu

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Differential gonadotropin releasing hormone (GnRH) expression, autoregulation and effects in two models of rat luteinized ovarian cells

Cited by 6 publications

References 52 publications

Lack of Functional GABA<sub>B</sub> Receptors Alters <b><i>Kiss1</i></b>, <b><i>Gnrh1 </i></b>and <b><i>Gad1</i></b> mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4

Lack of Functional GABA<sub>B</sub> Receptors Alters <b><i>Kiss1</i></b>, <b><i>Gnrh1 </i></b>and <b><i>Gad1</i></b> mRNA Expression in the Medial Basal Hypothalamus at Postnatal Day 4

Lack of functional GABAB receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Bir Köpekte Tek Taraflı Luteoma Olgusu

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Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain