Differential glucocorticoid effects on proliferation and invasion of human trophoblast cell lines

Mandl, Manuela; Ghaffari-Tabrizi, Nassim; Haas, J.; Nöhammer, G; Desoyé, Gernot

doi:10.1530/rep.1.00976

Cited by 54 publications

(37 citation statements)

References 65 publications

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“…In another study, BeWo and JEG-3 choriocarcinoma cell lines used as models for human trophoblast were cultured with another synthetic glucocorticoid triamcinolone acetonide (TA). TA altered the number of viable and dead cells as well as cyclin B1 expression levels shown by Western blotting and to a lesser extent, invasion of BeWo and JEG-3 cell lines determined by Matrigel invasion assay and by measuring the secretion (ELISA) of matrix-metalloproteinases (MMP-2, MMP-9) [97].…”

Section: Labyrinth Zonementioning

confidence: 96%

The Effects of Glucocorticoids on Fetal and Placental Development

Korgun¹,

Ozmen²,

Unek³

et al. 2012

Glucocorticoids - New Recognition of Our Familiar Friend

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Section: Labyrinth Zonementioning

confidence: 96%

The Effects of Glucocorticoids on Fetal and Placental Development

Korgun¹,

Ozmen²,

Unek³

et al. 2012

Glucocorticoids - New Recognition of Our Familiar Friend

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“…Therefore, it may be possible that the progranulin synthesized mainly in the syncytiotrophoblast in vivo stimulates the cell proliferation of the proliferating extravillous cytotrophoblast cells in a paracrine manner [20]. The reason why the effect was not observed in Jeg-3 cells is obscure, but this clearly shows the different behavior of these two cell lines, which has been observed also by other investigators [21][22][23].…”

Section: Discussionmentioning

confidence: 51%

Expression Pattern of Progranulin in the Human Placenta and Its Effect on Cell Proliferation in the Choriocarcinoma Cell Line BeWo

Stubert

Richter

Gerber

et al. 2011

Journal of Reproduction and Development

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Abstract. Expression of the glycoprotein progranulin has been recently identified in rodent trophoblast cells during early embryonic development. The aim of our study was to describe the expression pattern of progranulin in human placental tissue specimens by immunostaining. We further analyzed the influence of progranulin on invasion and migration of isolated first trimester villous trophoblast cells. The effect of progranulin on cell proliferation was investigated using the human choriocarcinoma derived cell lines BeWo and Jeg-3. Cells were tested with recombinant human progranulin at various concentrations (0.1, 0.2 and 1.0 μg/ml). The strongest expression of progranulin was observed in the villous trophoblast cells, particularly in the syncytiotrophoblast. The intensity of staining in these cells was higher in the first trimester than in the third trimester. In contrast, the staining of the extravillous trophoblast cells and of the villous and decidual stroma was only weak. Using an ELISA technique, we also detected progranulin in amniotic fluid of the early second trimester. Isolated human first trimester trophoblast cells also expressed and secreted progranulin. Progranulin significantly stimulated the cell proliferation of BeWo cells, but it did not influence the amount of trophoblast cell migration and invasion in vitro. Furthermore, it did not promote the cell proliferation of Jeg-3 cells. Our results suggested that progranulin, although it is mainly synthesized and secreted by villous trophoblast cells, may not primarily act on the villous trophoblast cells in a paracrine or autocrine manner. The observed effect of progranulin on cell proliferation in BeWo cells may indicate a growth stimulating effect also on the small part of proliferating extravillous trophoblast cells during placental development.

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“…The glucocorticoid re ceptor has been identified in primary trophoblast cells, as well as in the extravillous trophoblast cell line HTR-8/SVneo (20,21). It has been shown that one of the cell lines used in this study, JEG-3, expresses GR, while JAr cells were reported to lack the GR (22,23). However, an independent study has shown that some JAr cells contain low levels of GR, and therefore could be sensitive to glucocorticoid treatment (24).…”

Section: Discussionmentioning

confidence: 66%

Choriocarcinoma cell line Response to Dexamethasone

Bojić‐Trbojević¹,

Kolundžić²,

Petronijević³

et al. 2010

Journal of Medical Biochemistry

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Kratak sadr`aj: Horiokarcinomske }elijske linije JAr i JEG-3 su model sistemi za ispitivanje transformisanog trofo blasta. Obe }elijske linije eksprimiraju galektin-1, ~ija je pove}ana ekspresija pokazana u horiokarcinomima u odno su na normalni trofoblast trudno}e. U ovom radu ispitivan je efekat sintetskog glukokortikoida deksametazona na JAr i JEG-3 }elijske linije, upotrebom MTT testa, ELISA testa na }elijama i testovima adhezije i migracije. Broj `ivih }elija, kao indikator proliferacije }elija, zna ~ajno je pove}an nakon tretmana 0,1 i 1 nmol/L deksameta zonom, a proliferacija JEG-3 je zna~ajno pove}ana u ~itavom opsegu upotrebljenih koncentracija deksameta zo na (0,1-100 nmol/L). Galektin-1 je u JAr moduliran deksametazonom, koji blago, ali zna~ajno smanjuje pro duk ciju galektina-1 u niskim koncentracijama (0,1 i 1 nmol/L). Kod JEG-3 }elija, produkcija galektina-1 je zna~ajno smanjena samo nakon tretmana 100 nmol/L deksameta zonom. U prisustvu laktoze kao inhibitornog {e}era za galektin-1 adhezija JEG-3 }elija je zna~ajno pove}ana, dok deksa metazon uz to smanjuje i migraciju JEG-3 }elija. Rezultati dobijeni u ovom radu po kazuju da deksametazon uti~e na proliferaciju i u manjoj meri na galektin-1 kod JAr i JEG-3 }elija, na na~in spe ci fi~an za }elijsku liniju. Dobijeni podaci ukazuju na to da bi tret man glukokortikoidima in vivo mogao imati uticaja na }elije horiokarcinoma. Summary: Choriocarcinoma cell lines JAr and JEG-3 are model systems for the study of transformed trophoblast. Both cell lines were shown to produce galectin-1, expression of which was increased in choriocarcinoma when compared to the normal trophoblast of pregnancy. In this study the effects of synthetic glucocorticoid dexamethasone were inves ti gated in both JAr and JEG-3 cell lines by the MTT test, cell based ELISA, and the cell adhesion and migration tests. Viable cell number/cell proliferation of JAr cells was signi ficantly increased after treatment with 0.1 and 1 nmol/L of dexamethasone, while proliferation of JEG-3 cells was signi ficantly increased after treatment in the whole concentration range of dexamethasone (0.1-100 nmol/L). Galectin-1 in JAr cells was modulated by dexamethasone, which mildly, but significantly decreased production at low concentrations (0.1 and 1 nmol/L). In JEG-3 cells pro duction of galectin-1 was significantly decreased only after treat ment with 100 nmol/L of dexamethasone. Cell adhe sion of JEG-3 was significantly increased in the presence of lacto se, an inhibitory sugar for gal-1, while dexamethasone indu ced decrease of JEG-3 cell migration. These findings have shown that dexamethasone may affect proliferation, gal-1 production and cell migration, in a cell line specific manner. These data suggest that glucocorticoid treatment in vivo might have the potential to affect cell functions in cho riocarcinoma.

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Differential glucocorticoid effects on proliferation and invasion of human trophoblast cell lines

Cited by 54 publications

References 65 publications

The Effects of Glucocorticoids on Fetal and Placental Development

The Effects of Glucocorticoids on Fetal and Placental Development

Expression Pattern of Progranulin in the Human Placenta and Its Effect on Cell Proliferation in the Choriocarcinoma Cell Line BeWo

Choriocarcinoma cell line Response to Dexamethasone

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