Differential gene expression patterns in the autogamous plant Hordeum euclaston (Poaceae)

Abstract

ABSTRACT. Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carried out to investigate 12 strains at five ontogenetic stages, to determine the patterns of expression of these genes during development. The differences in the presence versus absence of certain isoenzyme bands may be due to differential regulatory activation in response to environmental differences, as all plants showed the same structural genes, although these genes were active in different tissues and/or times of development. These results indicate the importance of differential gene activation in the metabolic phenotype variability of this strictly autogamous, highly homozygous species. The same structural alleles for isoenzymes showed the active form of the enzymes (phenotypic expression) to be present in different tissues and/or stages of development. Differential isoenzyme gene activation was shown to be directly responsible for the enzymatic variability (metabolic phenotype) presented by the plants, which seem to possess almost no heterozygosis.

Chromosomal Localization and Contribution of Three Homoeologous Genes to Biosynthesis of Cytosolic Aspartate Aminotransferase in Common Wheat

“…Similar pattern was observed in case of different plants closely related to wheat as: maize [8], rice [9], millet [10], barley [11, 12] or finger millet [13]. Based on the zymographic analyses of AAT preparations from different subcellular fractions of wheat seedlings, it was found that AAT-1 zone (the one with the highest electrophoretic mobility towards the anode) is composed of mitochondrial allozymes, while AAT-2 and AAT-3 zones correspond to plastidial and cytosolic allozymes, respectively [7].…”

NJ cluster analysis of the SnRK2, PYR/PYL/RCAR, and ABF genes in Tibetan hulless barley

“…The following gene families have been identified and characterized in Oryza (SnRK2 family) (Saha et al, 2014), Arabidopsis (PYR/RCAR family) (Merilo et al, 2013), and Populus (ABF family) (Ji et al, 2013). Georg-Kraemer et al (2011) analyzed differential gene expression patterns in Hordeum euclaston. Zhou et al (2015) isolated a novel EsMcsu1 gene encoding a molybdenum cofactor from Eutrema salsugineum, which promotes the ABA biosynthesis and increases drought resistance in alfalfa.…”