Differential gene expression patterns and colocalization of ATP-gated P2X6/P2X4 ion channels during rat small intestine ontogeny

Padilla, Karla; Gonzalez-Mendoza, David; Berumen, Laura C.; Escobar-Cabrera, Jesica; Miledi, Ricardo; García-Alcocer, Guadulupe

doi:10.1016/j.gep.2016.08.002

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…The receptors P2X4 and P2X6 are expressed in the central nervous system, endothelial cells, placenta, and thymus [37,38]. Carbamazepine derivatives are potent agonists of the P2X4 receptor [39], and the allosteric modulator ivermectin increases ATP concentration and potentiates its effects [39].…”

Section: P2 Receptorsmentioning

confidence: 99%

“…ATP and 2-methylthio-ATP are full agonists for the P2X5 receptor, which in turn is inhibited by TNP-ATP, PPADS, and suramin [40,41]. The P2X6 receptor was discovered in rat brain and by homology [42], being only functionally expressed as a heteromultimer [38,43]. P2X7 receptor is expressed in immune cells, pancreas, skin, and microglia [35,44].…”

Section: P2 Receptorsmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of P2 receptors in hematopoiesis and hematopoietic disorders, and as pharmacological targets

Filippin

Souza

Júnior

et al. 2019

Purinergic Signalling

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Several reports have shown the presence of P2 receptors in hematopoietic stem cells (HSCs). These receptors are activated by extracellular nucleotides released from different sources. In the hematopoietic niche, the release of purines and pyrimidines in the milieu by lytic and nonlytic mechanisms has been described. The expression of P2 receptors from HSCs until maturity is still intriguing scientists. Several reports have shown the participation of P2 receptors in events associated with modulation of the immune system, but their participation in other physiological processes is under investigation. The presence of P2 receptors in HSCs and their ability to modulate this population have awakened interest in exploring the involvement of P2 receptors in hematopoiesis and their participation in hematopoietic disorders. Among the P2 receptors, the receptor P2X7 is of particular interest, because of its different roles in hematopoietic cells (e.g., infection, inflammation, cell death and survival, leukemias and lymphomas), making the P2X7 receptor a promising pharmacological target. Additionally, the role of P2Y12 receptor in platelet activation has been well-documented and is the main example of the importance of the pharmacological modulation of P2 receptor activity. In this review, we focus on the role of P2 receptors in the hematopoietic system, addressing these receptors as potential pharmacological targets.

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Section: P2 Receptorsmentioning

confidence: 99%

Section: P2 Receptorsmentioning

confidence: 99%

Involvement of P2 receptors in hematopoiesis and hematopoietic disorders, and as pharmacological targets

Filippin

Souza

Júnior

et al. 2019

Purinergic Signalling

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Colorectal Cancer and Purinergic Signalling: An Overview

Roliano

Azambuja

Brunetto

et al. 2022

Cancers

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Colorectal cancer (CRC) is among the most common cancers and exhibits a high fatality rate. Gut inflammation is related to CRC, with loss of homeostasis in immune cell activities. The cells of the innate and adaptive immune system, including macrophages, neutrophils, mast cells, and lymphocytes, are present in most solid tumors. Purinergic signaling allows for communication between immune cells within the tumor microenvironment (TME) and can alter the TME to promote tumor progression. This system is regulated by the availability of extracellular purines to activate purinoceptors (P1 and P2) and is tightly controlled by ectonucleotidases (E-NPP, CD73/CD39, ADA) and kinases, which interact with and modify nucleotides and nucleosides availability. In this review, we compiled articles detailing the relationship of the purinergic system with CRC progression. We found that increased expression of CD73 leads to the suppression of effector immune cell functions and tumor progression in CRC. The P1 family purinoceptors A1, A2A, and A2B were positively associated with tumor progression, but A2B resulted in increased cancer cell apoptosis. The P2 family purinoceptors P2X5, P2X7, P2Y2, P2Y6, and P2Y12 were factors primarily associated with promoting CRC progression. In summary, CD39/CD73 axis and the purinergic receptors exhibit diagnostic and prognostic value and have potential as therapeutic targets in CRC.

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Imperatorin Improves Obesity-Induced Cardiac Sympathetic Nerve Injury Mediated by P2X4 Receptor in Stellate Sympathetic Ganglion

Zhang

Wen

Liang

et al. 2023

IJMS

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Obesity can activate the inflammatory signal pathway, induce in the body a state of chronic inflammation, and increase the excitability of the sympathetic nervous system, which may induce sympathetic neuropathic injury. The stellate sympathetic ganglia (SG) can express the P2X4 receptor, and the abnormal expression of the P2X4 receptor is related to inflammation. Imperatorin (IMP) is a kind of furan coumarin plant which has anti-inflammatory effects. This project aimed to investigate whether IMP can affect the expression of P2X4 receptors in the SG of obese rats to display a protective effect from high-fat-triggered cardiac sympathetic neuropathic injury. Molecular docking through homology modelling revealed that IMP had good affinity for the P2X4 receptor. Our results showed that compared with the normal group, the administration of IMP or P2X4 shRNA decreased sympathetic excitement; reduced the serum levels of triglyceride, total cholesterol, and lactate dehydrogenase; downregulated the expression of P2X4 receptors in SG; and inhibited the expression of inflammatory factors in the SG and serum of obese rats significantly. In addition, the expression of factors associated with the cell pyroptosis GSDMD, caspase-1, NLRP-3, and IL-18 in obese rats were significantly higher than those of the normal rats, and such effects were decreased after treatment with IMP or P2X4 shRNA. Furthermore, IMP significantly reduced the ATP-activated currents in HEK293 cells transfected with P2X4 receptor. Thus, the P2X4 receptor may be a key target for the treatment of obesity-induced cardiac sympathetic excitement. IMP can improve obesity-induced cardiac sympathetic excitement, and its mechanism of action may be related to the inhibition of P2X4 receptor expression and activity in the SG, suppression of cellular pyroptosis in the SG, and reduction of inflammatory factor levels.

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Differential gene expression patterns and colocalization of ATP-gated P2X6/P2X4 ion channels during rat small intestine ontogeny

Cited by 3 publications

References 34 publications

Involvement of P2 receptors in hematopoiesis and hematopoietic disorders, and as pharmacological targets

Involvement of P2 receptors in hematopoiesis and hematopoietic disorders, and as pharmacological targets

Colorectal Cancer and Purinergic Signalling: An Overview

Imperatorin Improves Obesity-Induced Cardiac Sympathetic Nerve Injury Mediated by P2X4 Receptor in Stellate Sympathetic Ganglion

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