Differential gene expression pattern of neurotrophins and their receptors during neuronal differentiation of rat bone marrow stromal cells

Yaghoobi, Mohammad Mehdi; Mowla, Seyed Javad

doi:10.1016/j.neulet.2005.12.009

Cited by 47 publications

(20 citation statements)

References 24 publications

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“…Neuronal differentiation of rBMSCs was performed as described previously (Woodbury et al, 2002;Yaghoobi and Mowla, 2006) with modification. Briefly, rBMSCs were retrieved from sub-confluent culture by trypsinization, and plated at the density of 4 × 10 3 cells/cm 2 .…”

Section: Neuronal Induction Of Rbmscsmentioning

confidence: 99%

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Cho

Jang

et al. 2009

Exp Mol Med

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When we treated rat bone marrow stromal cells (rBMSCs) with neuronal differentiation induction media, typical unfolded protein response (UPR) was observed. BIP/GRP78 protein expression was time-dependently increased, and three branches of UPR were all activated. ATF6 increased the transcription of XBP1 which was successfully spliced by IRE1. PERK was phosphorylated and it was followed by eIF2α phosphorylation. Transcription of two downstream targets of eIF2α, ATF4 and CHOP/GADD153, were transiently up-regulated with the peak level at 24 h. Immunocytochemical study showed clear coexpression of BIP and ATF4 with NeuN and Map2, respectively. UPR was also observed during the neuro-

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Section: Neuronal Induction Of Rbmscsmentioning

confidence: 99%

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Cho

Jang

et al. 2009

Exp Mol Med

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“…Previous studies have demonstrated that, after chemical induction, BMSCs can significantly increase the expression of the genes associated with neural cells and express neuronal markers [8][9][10]18] . However, recently it has been suggested that morphological and phenotypic data cannot unequivocally indicate whether these cells are normally functioning neurons [19] .…”

Section: Discussionmentioning

confidence: 99%

Rat Bone Marrow Stromal Cells Express Retinal Phenotypic Markers Following Different Induction Protocols

Liu

Yin²,

Wu³

et al. 2009

Ophthalmic Res

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Purpose: To investigate and compare the effects of two different protocols on inducing bone marrow stromal cells (BMSCs) to differentiate into retinal neurons. Methods: BMSCs from adult rat femurs were cultured and passaged 3 times. The cells were then induced by either the basic fibroblast growth factor (bFGF) protocol or by co-culturing with neonatal rat retina. Morphological changes were monitored and immunocytochemistry was used to detect the expression of neuronal and retinal neuron-specific markers. Results: The bFGF protocol induced a maximum of 74% of the BMSCs to assume a neuronal-like morphology, but this was also associated with significant cell detachment during the 7-day culture period. By comparison, with the co-culture method only a maximum of 10% of cells became neuronal-like, but without marked cell detachment. The cells expressed microtubule-associated protein-2 (MAP-2, a neuronal marker) and Thy1.1 (a retinal ganglion cell marker) at all time points under both protocols. The percentage of MAP-2- and Thy1.1-positive cells was much higher following bFGF induction compared to co-culture induction. Following bFGF induction a small number of opsin-positive cells (a photoreceptor marker) were observed only on day 1. These cells were first observed at day 5 with co-culturing. The expression of protein kinase Cα (a bipolar cell marker) and glial fibrillary acidic protein was not detected after either protocol. Conclusion: BMSCs can express retinal neuron-specific phenotypic markers in response to bFGF induction or retinal co-cultures in vitro. When a short induction time is used the bFGF induction is superior, albeit with certain limitations, to retinal co-cultures for inducing BMSCs to express retinal neuron-specific markers.

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“…Currently, the most effectively characterized types of multipotent stem cells are from bone marrow. Bone marrow stem cells (BMSC) have been shown to differentiate into specialized cells, including hepatocytes [65][66][67] , neural cells [68][69][70][71] and mainly mesodermal derivatives such as bone, cartilage, cardiac muscles, skeletal muscle, and fat. If BMSC are placed on a proper bio-degradable scaffold and implanted, they can act as anti-fibrotic, angiogenic, antiapoptotic, and mitotic agents.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Mesenchymal Stem Cells for Cell Therapy and Tissue Regeneration in Urology

Shi¹,

Zhang²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

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Differential gene expression pattern of neurotrophins and their receptors during neuronal differentiation of rat bone marrow stromal cells

Cited by 47 publications

References 24 publications

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Rat Bone Marrow Stromal Cells Express Retinal Phenotypic Markers Following Different Induction Protocols

Mesenchymal Stem Cells for Cell Therapy and Tissue Regeneration in Urology

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