Differential gene expression of fatty acid binding proteins during porcine adipogenesis

Samulin, Johanna; Berget, Ingunn; Lien, Sigbjørn; Sundvold, Hilde

doi:10.1016/j.cbpb.2008.06.010

Cited by 68 publications

(51 citation statements)

References 53 publications

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“…Lycopene intake also significantly decreased the expression of PPARg and its target gene, FABP3, in the adrenal glands and kidney [Zaripheh et al, 2006]. FABP3 showed highly correlated expression patterns with porcine adipogenesis as well as expression patterns of PPARg [Samulin et al, 2008]. Therefore, FABP3 expression, a marker of adipogenesis, was also apparently regulated by PPARg [Xu et al, 2006].…”

Section: Discussionmentioning

confidence: 94%

“…In the current study, we showed that PPARg plays an important role in regulation process of FABP3 by Sirt1. However, recent study has suggested that PPARa expression has also been correlated to FABP3 expression (Samulin et al, 2008). Therefore, the exact mechanisms for the regulation of FABP3 by Sirt1 in the adipocytes are as yet unclear and require more in-depth investigation.…”

Section: Discussionmentioning

confidence: 99%

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Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Shan

Ren

et al. 2009

J of Cellular Biochemistry

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To investigate whether Sirt1 could modulate fatty acid-binding protein 3 (FABP3), we treated porcine adipocytes either with the Sirt1 inhibitor nicotinamide (NAM), with the Sirt1 activator resveratrol (RES), or by knockdown of Sirt1 by Sirt1-siRNA. NAM or knockdown with Sirt1-siRNA significantly inhibited Sirt1 mRNA expression, while increasing FABP3 mRNA levels. RES or RES + Sirt1-siRNA treatments further proved that Sirt1 negatively regulated FABP3 gene expression in adipocytes. We also found a similar Sirt1 regulation pattern for PPAR gamma to that of FABP3 in adipocytes. Furthermore, NAM/RES + PPAR gamma-siRNA treatments showed that Sirt1 may regulate the FABP3 gene expression partly through the PPAR gamma-mediated signals. In summary, Sirt1 regulates the expression of FABP3 gene in adipocytes, and PPAR gamma apparently plays an important role in this process.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Shan

Ren

et al. 2009

J of Cellular Biochemistry

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“…FABP1 delivers PPAR ligands to the nucleus to modulate PPAR-␣ and PPAR-␥ gene expression (16). More examples have been reported for FABP4 and -5 (7,15,17).…”

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confidence: 99%

Novel Functions of Lipid-binding Protein 5 in Caenorhabditis elegans Fat Metabolism

Joo

Paik

2011

Journal of Biological Chemistry

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The lipid-binding protein (LBP) family is conserved from Caenorhabditis elegans to mammals and essential for fatty acid homeostasis. RNAi-mediated knockdown of nine C. elegans lbp family members revealed that lbp-5 regulates fat accumulation. C. elegans LBP-5 bound directly to various fatty acids with varying affinities. lbp-5 expression in nhr-49(nr2041) worms was much lower than in N2 worms. Nhr-49 transcriptional activity also decreased with lbp-5 deletion, suggesting that they may work together as functional partners in fat metabolism. In support of this notion, LBP-5 translocated into nuclei, where it appeared to influence C. elegans NHR-49 target genes involved in energy metabolism. Interestingly, LBP-5 is required for stearic acid-induced transcription of NHR-49 target genes. Thus, this knowledge could help identify therapeutic targets to treat obesity and diseases associated with nematode-host interactions.

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“…Studies of primary SV cell cultures derived from 150-day-old pigs (as opposed to the typical 5-9-day-old pig) showed elevation of FABP3, FABP4, and FABP5 early in culture, with FABP3 and FABP4 increasing the most throughout adipogenesis (80). PPARg1 and PPARa, but not PPARg2, were elevated early, coupled with a modest increase in PPARa throughout culture (80,104).…”

Section: Preadipocyte Differentiationmentioning

confidence: 94%

Preadipocyte and Adipose Tissue Differentiation in Meat Animals: Influence of Species and Anatomical Location

Hausman¹,

Basu

Wei

et al. 2014

Annu. Rev. Anim. Biosci.

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Early in porcine adipose tissue development, the stromal-vascular (SV) elements control and dictate the extent of adipogenesis in a depot-dependent manner. The vasculature and collagen matrix differentiate before overt adipocyte differentiation. In the fetal pig, subcutaneous (SQ) layer development is predictive of adipocyte development, as the outer, middle, and inner layers of dorsal SQ adipose tissue develop and maintain layered morphology throughout postnatal growth of SQ adipose tissue. Bovine and ovine fetuses contain brown adipose tissue but SQ white adipose tissue is poorly developed structurally. Fetal adipose tissue differentiation is associated with the precocious expression of several genes encoding secreted factors and key transcription factors like peroxisome proliferator activated receptor (PPAR)γ and CCAAT/-enhancer-binding protein. Identification of adipocyte-associated genes differentially expressed by age, depot, and species in vivo and in vitro has been achieved using single-gene analysis, microarrays, suppressive subtraction hybridization, and next-generation sequencing applications. Gene polymorphisms in PPARγ, cathepsins, and uncoupling protein 3 have been associated with back fat accumulation. Genome scans have mapped several quantitative trait loci (QTL) predictive of adipose tissue-deposition phenotypes in cattle and pigs.

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Differential gene expression of fatty acid binding proteins during porcine adipogenesis

Cited by 68 publications

References 53 publications

Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Regulatory role of Sirt1 on the gene expression of fatty acid‐binding protein 3 in cultured porcine adipocytes

Novel Functions of Lipid-binding Protein 5 in Caenorhabditis elegans Fat Metabolism

Preadipocyte and Adipose Tissue Differentiation in Meat Animals: Influence of Species and Anatomical Location

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