Differential gene expression in the duodenum, jejunum and ileum among crossbred beef steers with divergent gain and feed intake phenotypes

Lindholm‐Perry, A. K.; Butler, Ashley; Kern, R.; Hill, R J; Kuehn, L. A.; Wells, John W.; Oliver, W. T.; Hales, K. E.; Foote, Andrew P; Freetly, H. C.

doi:10.1111/age.12440

Cited by 38 publications

(38 citation statements)

References 38 publications

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“…The animals with higher gains may be mitigating the effects of oxidative stress by reducing cellular levels of glutathione. Genes involved in oxidative stress have been previously identified in other tissues from these same animals [4,5,23,24]. A specific example of this includes the UQCRQ gene, which was identified as differentially expressed in the muscle tissue from these animals and displayed the same direction of expression in all cohorts [5].…”

Section: Genes/pathways Identified For Gainmentioning

confidence: 89%

Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers

et al. 2020

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Mesenteric fat is a visceral fat depot that increases with cattle maturity and can be influenced by diet. There may be a relationship between the accumulation of mesenteric fat and feed efficiency in beef cattle. The purpose of this study was to identify genes that may be differentially expressed in steers with high and low BW gain and feed intake. RNA-Seq was used to evaluate the transcript abundance of genes in the mesenteric fat from a total of 78 steers collected over 5 different cohorts. A meta-analysis was used to identify genes involved with gain, feed intake or the interaction of both phenotypes. The interaction analysis identified 11 genes as differentially expressed. For the main effect of gain, a total of 87 differentially expressed genes (DEG) were identified (P ADJ <0.05), and 24 were identified in the analysis for feed intake. Genes identified for gain were involved in functions and pathways including lipid metabolism, stress response/protein folding, cell proliferation/growth, axon guidance and inflammation. The genes for feed intake did not cluster into pathways, but some of the DEG for intake had functions related to inflammation, immunity, and/or signal transduction (JCHAIN, RIPK1, LY86, SPP1, LYZ, CD5, CD53, SRPX, and NF2). At P ADJ <0.1, only 4 genes (OLFML3, LOC100300716, MRPL15, and PUS10) were identified as differentially expressed in two or more cohorts, highlighting the importance of evaluating the transcriptome of more than one group of animals and incorporating a meta-analysis. This meta-analysis has produced many mesenteric fat DEG that may be contributing to gain and feed intake in cattle. OPEN ACCESSCitation: Lindholm-Perry AK, Freetly HC, Oliver WT, Rempel LA, Keel BN (2020) Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers. PLoS ONE 15(1): e0227154. https://

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Section: Genes/pathways Identified For Gainmentioning

confidence: 89%

Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers

et al. 2020

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“…To assess the differential expression of genes in the mesenteric fat of steers, the Affymetrix GeneAtlas System in conjunction with Bovine 1.1ST array strips were used, as described by Lindholm‐Perry et al . (). These arrays evaluate 24 341 genes using 526 810 probes.…”

Section: Methodsmentioning

confidence: 97%

“…; Lindholm‐Perry et al . ). Intestinal inflammation in human conditions such as ulcerative colitis and Crohn's disease have a relationship with the amount of mesenteric fat, suggesting possible cellular communication between these two tissues (Drouet et al .…”

Section: Introductionmentioning

confidence: 97%

Relationships between the genes expressed in the mesenteric adipose tissue of beef cattle and feed intake and gain

et al. 2017

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Mesenteric fat, a depot within the visceral fat, accumulates in cattle during maturation and finishing and may be a potential source of production inefficiency. The aim of this study was to determine whether the genes expressed in the mesenteric fat of steers were associated with body weight gain and feed intake. Sixteen steers chosen by their rank of distance from the bivariate mean for gain and feed intake were used for this study. Mesenteric fat was obtained and evaluated for differences in gene expression. A total of 1831 genes were identified as differentially expressed among steers with variation in feed intake and gain. Many of these genes were involved with metabolic processes such as proteolysis, transcription and translation. In addition, the Gene Ontology annotations including transport and localization were both over-represented among the differentially expressed genes. Pathway analysis was also performed on the differentially expressed genes. The superoxide radical degradation pathway was identified as over-represented based on the differential expression of the genes GPX7, SOD2 and TYRP1, suggesting a potential role for oxidative stress or inflammatory pathways among low gain-high intake animals. GPX7 and SOD2 were in lower transcript abundance, and TYRP1 was higher in transcript abundance among the low gain-high feed intake animals. The retinoate biosynthesis pathway was also enriched due to the differential expression of the genes AKR1C3, ALDH8A1, RDH8, RDH13 and SDR9C7. These genes were all more highly expressed in the low gain-high intake animals. The glycerol degradation and granzyme A signaling pathways were both associated with gain. Three glycerol kinase genes and the GZMA gene were differentially expressed among high vs. low gain animals. Mesenteric fat is a metabolically active tissue, and in this study, genes involved in proteolysis, transcription, translation, transport immune function, glycerol degradation and oxidative stress were differentially expressed among beef steers with variation in body weight gain and feed intake.

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“…; Lindholm‐Perry et al . ). This study was performed using a more targeted assay to determine whether some of those same genes were involved in gain in an unrelated population of steers.…”

Section: Descriptive Statistics For Initial Body Weight (Bw) Averagementioning

confidence: 97%

“…) than in those under normal, healthy circumstances (Lindholm‐Perry et al . ), and the animals chosen for this study showed no signs of illness.…”

Section: Descriptive Statistics For Initial Body Weight (Bw) Averagementioning

confidence: 99%

Relationships between inflammation‐ and immunity‐related transcript abundance in the rumen and jejunum of beef steers with divergent average daily gain

et al. 2017

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The bovine rumen papillae are in contact with a wide array of microorganisms and the metabolites they produce, which may activate an inflammatory and/or immune response. Cytokines, chemokines and their receptor genes were tested for differential expression in the rumen and jejunum of beef steers with greater and lesser average daily body weight gain (ADG) near the average daily dry matter intake (DMI) for the population. Angus-sired steers (n = 16) were used to represent the greater (ADG = 2.2 ± 0.07 kg/day; DMI = 10.1 ± 0.05 kg/day) and lesser (ADG = 1.7 ± 0.05 kg/day; DMI = 10.1 ± 0.05 kg/day) ADG groups with eight steers each. Rumen epithelium and jejunum mucosal samples were collected at slaughter, and gene expression was evaluated using a commercially available qRT-PCR array containing 84 genes representing chemokines, cytokines and their receptors. None of the genes on the array were differentially expressed in the jejunum of the steers with greater vs. lesser ADG. However, in the rumen, two chemokine genes (CCL11, CXCL5) and one receptor gene (IL10RA) were detected as differentially expressed (P < 0.05). The genes IL1A, BMP2, CXCL12 and TNFSF13 also displayed trends for differential expression (P < 0.10). All of the genes identified were lower in transcript abundance in the greater ADG animals. Thus, greater ADG steers have a lesser inflammatory response in the rumen papillae, which may lead to a more efficient use of nutrients.

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Differential gene expression in the duodenum, jejunum and ileum among crossbred beef steers with divergent gain and feed intake phenotypes

Cited by 38 publications

References 38 publications

Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers

Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers

Relationships between the genes expressed in the mesenteric adipose tissue of beef cattle and feed intake and gain

Relationships between inflammation‐ and immunity‐related transcript abundance in the rumen and jejunum of beef steers with divergent average daily gain

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