Differential gene expression in the small intestines of wildtype and W/W^V mice

Abstract

Seven known genes were differentially expressed in wildtype and W/W(V) mice. COX7B (cytochrome c oxidase, subunit VIIb) and SORCIN (encoding multidrug-resistance complex, class 4) were suppressed in both fed and fasted W/W(V) mice. Expression of another five genes was increased in W/W(V) mice: ADA (adenosine deaminase), MDH1 (malate dehydrogenase), RPL-8 (ribosomal protein L8), SPTB2 (spectrin, nonerythroid, beta subunit), and p6-5 (encoding phosphorylcholine [PC] T-cell suppressor factor [TsF]). Differential expression was the same in fasted and fed animals, suggesting that the differences were independent of the dietetic state. We conclude that several genes are differentially expressed in the small intestines of W/W(V) mice where the major lesion is loss of pacemaker ICC. Differential gene display may help develop a molecular profile of motility disorders in which ICC are lost.

Absence of peristalsis in the ileum of W/WV mutant mice that are selectively deficient in myenteric interstitial cells of Cajal

“…1B). These results are similar to those reported in previous studies (Malysz et al, 1996;Takayama et al, 2001Takayama et al, , 2002Nakagawa et al, 2005).…”

“…Furthermore, it has been reported that smooth muscle preparations of the small intestine (duodenum, jejunum or ileum) of W/W V mutant mice show a variety of electrical activities (Malysz et al, 1996), longitudinal cyclic contractions (Daniel et al, 2002) and distension-induced peristalsis . In the small intestine of W/W V mutant mice, while ICC-MY are absent, the ICC of the deep muscular plexus region (ICC-DMP) are present (Malysz et al, 1996;Takayama et al, 2001Takayama et al, , 2002. Recent studies reported the presence of synapse-like contacts between enteric nerve terminals and intramuscular ICC (ICC-IM) in the stomach, where ICC-MY have been shown to be the pacemaker ICC (Ward et al, 2000(Ward et al, , 2004.…”

Changes in contractile and electrical activity in the ileum of DSS-induced colitis model W/Wv mutant mice

“…We confirmed that there was no c-Kit positive immunoreactivity for ICC-MY in the ileum of W/W v DSS (-) mice, although there was for ICC-MY in the ileum of WT DSS (-) mice, as reported in previous studies (Malysz et al, 1996;Takayama et al, 2001;Nakagawa et al, 2005) (data not shown). There were no differences in the distribution of nNOS positive immunoreactive nerve fibers between WT DSS (-) mice and WT DSS (+) mice (n=2 each) in the ICC-DMP region of the circular smooth muscle (Fig.…”

Isolation of novel mouse genes that were differentially expressed in W/W V mouse fundus

“…We pursued the hypothesis that such genes might show differential expression in the GI tracts of wild-type mice and W/W V mice. [3][4][5][6] To determine the differential expression, an extensive analysis was performed to examine gene expression in the fundus of wild-type and W/W V mice. We identified dozens of differentially expressed unannotated transcripts, whose sequences were available only as expressed sequence tags (ESTs).…”