Differential gene expression in sugarcane leaf and internodal tissues of varying maturity

Carson, D.L.; Huckett, B. I.; Botha, Frederik C.

doi:10.1016/s0254-6299(15)30370-7

Cited by 22 publications

(24 citation statements)

References 29 publications

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“…A number of these motifs were also detected by Moyle and Birch (2013a, b) in the ScLSG promoter. The presence of these motifs is consistent with previous reports that transcripts that were up-regulated in the maturing stalk frequently had potential roles in defence or response to stresses (Carson et al 2002b;Casu et al 2004;Iskandar et al 2011). The highly conserved TC element was found approximately where a TATA box might generally be expected to be; it is interesting to note that the presence of this element in a promoter is associated with conditionspecific expression (Bernard et al 2010).…”

Section: Discussionsupporting

confidence: 89%

Tissue-specific transcriptome analysis within the maturing sugarcane stalk reveals spatial regulation in the expression of cellulose synthase and sucrose transporter gene families

et al. 2015

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Sugarcane (Saccharum spp. hybrids) accumulates high concentrations of sucrose in its mature stalk and a considerable portion of carbohydrate metabolism is also devoted to cell wall synthesis and fibre production. We examined tissue-specific expression patterns to explore the spatial deployment of pathways responsible for sucrose accumulation and fibre synthesis within the stalk. We performed expression profiling of storage parenchyma, vascular bundles and rind dissected from a maturing stalk internode of sugarcane, identifying ten cellulose synthase subunit genes and examining significant differences in the expression of their corresponding transcripts and those of several sugar transporters. These were correlated with differential expression patterns for transcripts of genes encoding COBRA-like proteins and other cell wall metabolism-related proteins. The sugar transporters genes ShPST2a, ShPST2b and ShSUT4 were significantly up-regulated in storage parenchyma while ShSUT1 was up-regulated in vascular bundles. Two co-ordinately expressed groups of cell wall related transcripts were also identified. One group, associated with primary cell wall synthesis (ShCesA1, ShCesA7, ShCesA9 and Shbk2l3), was up-regulated in parenchyma. The other group, associated with secondary cell wall synthesis (ShCesA10, ShCesA11, ShCesA12 and Shbk-2), was up-regulated in rind. In transformed sugarcane plants, the ShCesA7 promoter conferred stable expression of green fluorescent protein preferentially in the storage parenchyma of the maturing stalk internode. Our results indicate that there is spatial separation for elevated expression of these important targets in both sucrose accumulation and cell wall synthesis, allowing for increased clarity in our understanding of sucrose transport and fibre synthesis in sugarcane.

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Section: Discussionsupporting

confidence: 89%

Tissue-specific transcriptome analysis within the maturing sugarcane stalk reveals spatial regulation in the expression of cellulose synthase and sucrose transporter gene families

et al. 2015

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“…A survey of thousands of transcript sequences from maturing sugarcane culms revealed that transcripts for sugar metabolizing enzymes are relatively rare in maturing culms while transcripts for sugar transporters are very abundant (Carson et al, 2002; Casu et al, 2003). Also, mRNAs for a ShSUT1 transporter gene of a sugarcane hybrid were abundant in both source leaves and sink stems actively accumulating sucrose (Rae et al, 2005a, b).…”

Section: Sucrose Accumulation In Sugarcanementioning

confidence: 99%

Carbon partitioning in sugarcane (Saccharum species)

Wang¹,

Nayak²,

Koch³

et al. 2013

Front. Plant Sci.

140

100

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Focus has centered on C-partitioning in stems of sugarcane (Saccharum sp.) due to their high-sucrose accumulation features, relevance to other grasses, and rising economic value. Here we review how sugarcane balances between sucrose storage, respiration, and cell wall biosynthesis. The specific topics involve (1) accumulation of exceptionally high sucrose levels (up to over 500 mM), (2) a potential, turgor-sensitive system for partitioning sucrose between storage inside (cytosol and vacuole) and outside cells, (3) mechanisms to prevent back-flow of extracellular sucrose to xylem or phloem, (4) apparent roles of sucrose-P-synthase in fructose retrieval and sucrose re-synthesis, (5) enhanced importance of invertases, and (6) control of C-flux at key points in cell wall biosynthesis (UDP-glucose dehydrogenase) and respiration (ATP- and pyrophosphate-dependent phosphofructokinases). A combination of emerging technologies is rapidly enhancing our understanding of these points and our capacity to shift C-flux between sucrose, cell wall polymers, or other C-sinks.

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“…One interesting observation that was made in early gene expression profiling studies of the maturing sugarcane culm was that, despite the high concentrations of sucrose that accumulate in this tissue, transcripts that have functions related to sucrose metabolism are present at low abundance, and do not necessarily increase in abundance with sucrose concentration (Carson et al 2002;Casu et al 2003). The current model for sucrose accumulation suggests that sucrose moves from the phloem to the storage parenchyma via the symplast and that tight regulation of the cellular and sub-cellular partitioning of sugars is critical (Rae et al 2005b).…”

Section: Gene Expression Profiling In Sugarcanementioning

confidence: 99%