Differential gene expression in Poecilia vivipara exposed to diesel oil water accommodated fraction

Mattos, Jacó Joaquim; Siebert, Marília Nardelli; Lüchmann, Karim Hahn; Granucci, Ninna; Dorrington, Tarquin; Stoco, Patrícia Hermes; Grisard, Edmundo C.; Bainy, Afonso Celso Dias

doi:10.1016/j.marenvres.2009.11.002

Cited by 19 publications

(8 citation statements)

References 7 publications

(7 reference statements)

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“…Previous work on individual PAHs indicates that these substances can act as estrogen receptor agonists possibly due to cross-talk between the AhR- and ER-mediated pathways. ,, The significant and nearly significant differences compared to controls observed in these estrogen-responsive gene transcripts (i.e., increase in vepg mRNA in male livers, decrease of cyp19 mRNA in female caudal fin, and near significant decrease of vtg mRNA in female liver) are suggestive that there was a perturbation in the estrogen signaling pathway. Previous work using subtractive suppressive hybridization on the liver of female guppies, Poecilia vivipara , after a 24 h exposure to 10% (v/v) diesel WAF identified 27 differentially expressed gene fragments that included increased cyp1a and decreased vtg consistent with our findings.…”

Section: Resultssupporting

confidence: 89%

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Evaluation of Gene Bioindicators in the Liver and Caudal Fin of Juvenile Pacific Coho Salmon in Response to Low Sulfur Marine Diesel Seawater-Accommodated Fraction Exposure

Imbery

Buday

Miliano

et al. 2019

Environ. Sci. Technol.

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Low sulfur marine diesel (LSMD) is frequently involved in coastal spills and monitoring ecosystem damage, and the effectiveness of cleanup methods remains a challenge. The present study investigates the concentration and composition of polycyclic aromatic hydrocarbons (PAHs) dispersed in LSMD seawater accommodated fractions (WAFs) and assesses the effects of exposure on juvenile coho salmon (Onchorhynchus kisutch). Three WAFs were prepared with 333, 1067, and 3333 mg/L LSMD. The sum of 50 common PAHs and alkylated PAHs (tPAH50) measured by gas chromatography/triple quadrupole mass spectrometry showed saturation at ∼90 mg/L for all WAFs. These WAFs were diluted 30% for 96 h fish exposures. qPCR was performed on liver and caudal fin from the same genotypically sexed individuals to evaluate PAH exposure, general and oxidative stress, estrogenic activity, and defense against metals. Excluding metal response, our analyses reveal significant changes in gene expression following WAF exposure on juvenile salmon with differential sensitivity between males and females. The 3-methylcholanthrene responsive cytochrome P450-1a (cyp1a) transcript exhibited the greatest increase in transcript abundance in the caudal fin (10− 18-fold) and liver (6−10-fold). This demonstrates that cyp1a is a robust, sex-independent bioindicator of oil exposure in caudal fin, a tissue that is amenable to nonlethal sampling.

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Section: Resultssupporting

confidence: 89%

“…For example, there is some suggestion that diesel exposure can affect immune system components and metabolic functioning in guppies and coral reef fish. 63,65 Further in-depth RNA-seq analyses are in progress to identify additional transcripts and pathways affected in the liver and caudal fin upon LSMD WAF exposure.…”

Section: Environmental Science and Technologymentioning

confidence: 99%

Evaluation of Gene Bioindicators in the Liver and Caudal Fin of Juvenile Pacific Coho Salmon in Response to Low Sulfur Marine Diesel Seawater-Accommodated Fraction Exposure

Imbery

Buday

Miliano

et al. 2019

Environ. Sci. Technol.

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“…Consequently, P. nobilis along with two other benthic invertebrates (Cerastoderma glaucum and Ostrea edulis) has shown higher bioaccumulation of hydrocarbons in locations close to port and wastewater effluents (León et al, 2013) reflecting effects on the physiognomy of these organisms. In addition, diesel seems to potentially influence gene expression in fishes (Mattos et al, 2010). Also Nayar et al (2004) stated that accidental spillage of motorboat fuel affects negatively periphytic algal biomass.…”

Section: Discussionmentioning

confidence: 99%

Evaluating stable isotopic signals in bivalve Pinna nobilis under different human pressures

Alomar

Vázquez‐Luis

Magraner

et al. 2015

Journal of Experimental Marine Biology and Ecology

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“…Conversely, CCO activity was significantly elevated among floc exposed P. biscaya (lfc 1.9, adj. p-value 0.005; DeLeo et al, 2018), and among the liver/gills of fish following longer exposures (24 and 96 h) to WAF-oil, alone or dispersed (Cohen et al, 2001;Mattos et al, 2010). Elevated CCO activity among these animals may be associated with ongoing oxidative stress responses during short-term exposures to oil WAFs, that could ultimately lead to the hypoxic signature observed in our study.…”

Section: Bulk Exposure Seriesmentioning

confidence: 60%

Transcriptomic Responses of Deep-Sea Corals Experimentally Exposed to Crude Oil and Dispersant

et al. 2021

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Resource extraction from the ocean comes with ecosystem-wide risks, including threats to its biota such as the habitat forming corals that support elevated biomass and biodiversity. Despite catastrophic incidents like the Deepwater Horizon oil spill (DWHOS) disaster that occurred in 2010, offshore oil and gas drilling continues to occur around the world. Previous work investigating the toxicity of oil and the chemical dispersant used in an attempt to mitigate the effects of the DWHOS revealed that the dispersant elicits a stronger, negative physiological response than oil alone. However, little is known regarding the specific ways in which these anthropogenic pollutants impact organisms at the cellular level. To investigate the impacts of each pollutant and their synergistic effects on corals, we analyzed the transcriptional responses of the deep-sea octocorals Callogorgia delta and Paramuricea type B3 following 12 h of exposure to oil, dispersant, and mixtures of oil and dispersant. Analyses revealed that the highest levels of significant differential gene expression were found among the treatments containing dispersant, which corresponds to the significant effects observed in physiological experiments. Functional analyses of annotated transcripts further suggest both species- and colony-specific responses to the exposures, likely due to underlying cellular and physiological differences. However, some commonalities were observed among the responses to chemical stress across treatments and species, including immune and cellular stress responses, altered energy metabolism, and oxidative stress, elucidating how corals respond to chemical pollutants. As resource extraction is an ongoing threat, this study demonstrates the importance of considering the varied and diverse responses of biota to anthropogenic disturbances and the implications of introducing chemicals into vulnerable ecosystems like those associated with deep-sea corals.

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Differential gene expression in Poecilia vivipara exposed to diesel oil water accommodated fraction

Cited by 19 publications

References 7 publications

Evaluation of Gene Bioindicators in the Liver and Caudal Fin of Juvenile Pacific Coho Salmon in Response to Low Sulfur Marine Diesel Seawater-Accommodated Fraction Exposure

Evaluation of Gene Bioindicators in the Liver and Caudal Fin of Juvenile Pacific Coho Salmon in Response to Low Sulfur Marine Diesel Seawater-Accommodated Fraction Exposure

Evaluating stable isotopic signals in bivalve Pinna nobilis under different human pressures

Transcriptomic Responses of Deep-Sea Corals Experimentally Exposed to Crude Oil and Dispersant

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