Differential gene expression in neural stem cells and oligodendrocyte precursor cells: A cDNA microarray analysis

Hu, Jian; Fu, Sai-Li; Zhang, Kai Hua; Li, Ying; Yin, Lan; Lu, Pei-Hua; Xu, Xiao–Ming

doi:10.1002/jnr.20317

Cited by 49 publications

(45 citation statements)

References 44 publications

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“…Thus, our finding that high expression of Tf in OLs is consistent with previous report. In our previous study, we have shown that there was a 3-fold increase in ApoD expression in OLs as compared to NSCs (Hu et al 2004). Here, ApoD expression in Ols was found increasing more than 4-fold (but not type II astrocytes) as compared to OPCs.…”

Section: Candidate Genes That May Intrinsically Control Ol-specific Dsupporting

confidence: 47%

“…The high expression of HAMMR on OPCs implicated that HAMMR might be another CD44-like receptor which can mediate hyaluronan to maintain self-renewal of OPCs. Our previous study demonstrated that platelet derived growth factor receptor alpha polypeptide (PDGFRα) is differentially expressed (12-fold) during the induction from NSCs to OPCs (Hu et al 2004). Further study showed that PDGF-AA plays a dual role in mediating the number of OL lineage cells: an early instruction of OPC differentiation from NSCs and later proliferation of committed OPCs (Hu et al 2008).…”

Section: Gene Profiles Of Opcsmentioning

confidence: 99%

“…Microarray technology provides a powerful tool for large-scale gene expression studies (Hu et al 2004). In the present study, we cultured OPCs isolated from the spinal cords of embryonic rats at 16 days of gestation and induced them into OLs or type II astrocytes under different culture conditions, respectively.…”

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confidence: 99%

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Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Wang

Zhou

et al. 2011

Tohoku J. Exp. Med.

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Oligodendrocyte precursor cells (OPCs) are bipotential progenitor cells that can differentiate into myelin-forming oligodendrocytes or functionally undetermined type II astrocytes. Transplantation of OPCs is an attractive therapy for demyelinating diseases. However, due to their bipotential differentiation potential, the majority of OPCs differentiate into astrocytes at transplanted sites. It is therefore important to understand the molecular mechanisms that regulate the transition from OPCs to oligodendrocytes or astrocytes. In this study, we isolated OPCs from the spinal cords of rat embryos (16 days old) and induced them to differentiate into oligodendrocytes or type II astrocytes in the absence or presence of 10% fetal bovine serum, respectively. RNAs were extracted from each cell population and hybridized to GeneChip with 28,700 rat genes. Using the criterion of fold change > 4 in the expression level, we identified 83 genes that were up-regulated and 89 genes that were down-regulated in oligodendrocytes, and 92 genes that were up-regulated and 86 that were down-regulated in type II astrocytes compared with OPCs. The up-regulated genes, such as activating transcription factor 3 and myelin basic protein in oligodendrocytes or claudin 11 in type II astrocytes, might contribute to OPC differentiation and represent constitutive components of oligodendrocytes or type II astrocytes. The down-regulated genes in both oligodendrocytes and type II astrocytes, such as transcription factor 19, might be involved in maintaining self-renewal and/or represent the property of OPCs. These results provide new insights into the elucidation of the molecular mechanisms, by which OPCs differentiate to oligodendrocytes or type II astrocytes.

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Section: Candidate Genes That May Intrinsically Control Ol-specific Dsupporting

confidence: 47%

Section: Gene Profiles Of Opcsmentioning

confidence: 99%

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Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Wang

Zhou

et al. 2011

Tohoku J. Exp. Med.

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“…The list of 1,233 probe sets was then sorted by function, which identified 145 genes (174 probe sets) involved in development (SI Table 2). Analysis of this sublist, with special regard to genes involved in OL differentiation (24), OPC self-renewal (25,26), differentiation of NSCs to OPCs (27), OPC plasticity (10), or NSC self-renewal (11,12,28), revealed that eight of the most highly down-regulated genes upon treatment with TSA are associated with the differentiation of OPCs to OLs (Table 1). Interestingly, this group exhibited expression profiles nearly identical to those of BMP-2-treated OPCs and included proteins involved in myelin formation (MBP, MAG, PLP, OMG, and CNPase) (24) as well as other factors that promote the transition of OPCs to OLs (Nkx2.2, Sox10, and Fyn) (24,29,30).…”

Section: -L)mentioning

confidence: 99%

Inhibition of histone deacetylase activity induces developmental plasticity in oligodendrocyte precursor cells

Lyssiotis

Walker

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

116

103

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Recently, it was demonstrated that lineage-committed oligodendrocyte precursor cells (OPCs) can be converted to multipotent neural stem-like cells, capable of generating both neurons and glia after exposure to bone morphogenetic proteins. In an effort to understand and control the developmental plasticity of OPCs, we developed a high-throughput screen to identify novel chemical inducers of OPC reprogramming. Using this system, we discovered that inhibition of histone deacetylase (HDAC) activity in OPCs acts as a priming event in the induction of developmental plasticity, thereby expanding the differentiation potential to include the neuronal lineage. This conversion was found to be mediated, in part, through reactivation of sox2 and was highly reproducible at the clonal level. Further, genome-wide expression analysis demonstrated that HDAC inhibitor treatment activated sox2 and 12 other genes that identify or maintain the neural stem cell state while simultaneously silencing a large group of oligodendrocyte lineage-specific genes. This series of experiments demonstrates that global histone acetylation, induced by HDAC inhibition, can partially reverse the lineage restriction of OPCs, thereby inducing developmental plasticity.cell-based screen ͉ neural stem cells ͉ Sox2

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“…An alternative to genetic screening is to use recently developed gene profiling methods. Previous gene profiling studies of OPCs and OLs have focused on either the process of demyelination versus remyelination in CNS tissue derived from cuprizonedemyelinated animals (Jurevics et al, 2002;Arnett et al, 2003), or on the differences between uncommitted neural precursors and OPCs (Hu et al, 2004), as opposed to the terminal differentiation step of primary OPCs into mature OLs.…”

Section: Introductionmentioning

confidence: 99%

Functional Genomic Analysis of Oligodendrocyte Differentiation

Dugas¹,

Tai²,

Speed³

et al. 2006

J. Neurosci.

294

353

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To better understand the molecular mechanisms governing oligodendrocyte (OL) differentiation, we have used gene profiling to quantitatively analyze gene expression in synchronously differentiating OLs generated from pure oligodendrocyte precursor cells in vitro. By comparing gene expression in these OLs to OLs generated in vivo, we discovered that the program of OL differentiation can progress normally in the absence of heterologous cell-cell interactions. In addition, we found that OL differentiation was unexpectedly prolonged and occurred in at least two sequential stages, each characterized by changes in distinct complements of transcription factors and myelin proteins. By disrupting the normal dynamic expression patterns of transcription factors regulated during OL differentiation, we demonstrated that these sequential stages of gene expression can be independently controlled. We also uncovered several genes previously uncharacterized in OLs that encode transmembrane, secreted, and cytoskeletal proteins that are as highly upregulated as myelin genes during OL differentiation. Last, by comparing genomic loci associated with inherited increased risk of multiple sclerosis (MS) to genes regulated during OL differentiation, we identified several new positional candidate genes that may contribute to MS susceptibility. These findings reveal a previously unexpected complexity to OL differentiation and suggest that an intrinsic program governs successive phases of OL differentiation as these cells extend and align their processes, ensheathe, and ultimately myelinate axons.

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Differential gene expression in neural stem cells and oligodendrocyte precursor cells: A cDNA microarray analysis

Cited by 49 publications

References 44 publications

Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Differential Gene Expression in Oligodendrocyte Progenitor Cells, Oligodendrocytes and Type II Astrocytes

Inhibition of histone deacetylase activity induces developmental plasticity in oligodendrocyte precursor cells

Functional Genomic Analysis of Oligodendrocyte Differentiation

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