Differential gene expression in kernels and silks of maize lines with contrasting levels of ear rot resistance after Fusarium verticillioides infection

Lanubile, Alessandra; Pasini, Luca; Marocco, Adriano

doi:10.1016/j.jplph.2010.05.015

Cited by 66 publications

(42 citation statements)

References 40 publications

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“…These results indicate that resistance may be primarily due to constitutive defense mechanisms that prevent fungal infection (Campos-Bermudez et al, 2013). A similar result was obtained by studying resistant and susceptible maize genotypes (Alessandra et al, 2010). The assayed defense-related genes were found to be transcribed at high levels in the resistant line before infection, whereas these genes were expressed at basal levels in the susceptible line.…”

Section: Ear Rot and Stalk Rotsupporting

confidence: 72%

“Omics” of Maize Stress Response for Sustainable Food Production: Opportunities and Challenges

Gong

Yang

Tai

et al. 2014

OMICS: A Journal of Integrative Biology

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Maize originated in the highlands of Mexico approximately 8700 years ago and is one of the most commonly grown cereal crops worldwide, followed by wheat and rice. Abiotic stresses (primarily drought, salinity, and high and low temperatures), together with biotic stresses (primarily fungi, viruses, and pests), negatively affect maize growth, development, and eventually production. To understand the response of maize to abiotic and biotic stresses and its mechanism of stress tolerance, high-throughput omics approaches have been used in maize stress studies. Integrated omics approaches are crucial for dissecting the temporal and spatial systemlevel changes that occur in maize under various stresses. In this comprehensive analysis, we review the primary types of stresses that threaten sustainable maize production; underscore the recent advances in maize stress omics, especially proteomics; and discuss the opportunities, challenges, and future directions of maize stress omics, with a view to sustainable food production. The knowledge gained from studying maize stress omics is instrumental for improving maize to cope with various stresses and to meet the food demands of the exponentially growing global population. Omics systems science offers actionable potential solutions for sustainable food production, and we present maize as a notable case study.

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Section: Ear Rot and Stalk Rotsupporting

confidence: 72%

“Omics” of Maize Stress Response for Sustainable Food Production: Opportunities and Challenges

Gong

Yang

Tai

et al. 2014

OMICS: A Journal of Integrative Biology

View full text Add to dashboard Cite

show abstract

“…Abiotic stress, such as drought, can have a negative impact on the defense response (Wotton and Strange, 1987; Duke and Doehlert, 1996; Luo et al, 2010). Also, inherently low expression of defense and defense-associated genes may predispose the plant to greater infection (Chen et al, 2001; Alessandra et al, 2010). Genes expressed during infection may not necessarily be involved in resistance and could be causing increased susceptibility to fungal disease.…”

Section: Discussionmentioning

confidence: 99%

“…Studies investigating plant gene expression during pathogen attack have found the defense response goes beyond PR-proteins and involves transcription changes in both primary and secondary plant metabolic pathways and detoxification pathways (Boddu et al, 2007; Doehlemann et al, 2008; Alessandra et al, 2010). Phytohormones like salicyclic acid (SA), jasmonic acid (JA), ethylene (ET) have long been known to be an integral part of the defense response (Glazebrook, 2005; Jones and Dangl, 2006; Robert-Seilaniantz et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Aspergillus flavus infection induces transcriptional and physical changes in developing maize kernels

et al. 2014

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Maize kernels are susceptible to infection by the opportunistic pathogen Aspergillus flavus. Infection results in reduction of grain quality and contamination of kernels with the highly carcinogenic mycotoxin, aflatoxin. To understanding host response to infection by the fungus, transcription of approximately 9000 maize genes were monitored during the host-pathogen interaction with a custom designed Affymetrix GeneChip® DNA array. More than 4000 maize genes were found differentially expressed at a FDR of 0.05. This included the up regulation of defense related genes and signaling pathways. Transcriptional changes also were observed in primary metabolism genes. Starch biosynthetic genes were down regulated during infection, while genes encoding maize hydrolytic enzymes, presumably involved in the degradation of host reserves, were up regulated. These data indicate that infection of the maize kernel by A. flavus induced metabolic changes in the kernel, including the production of a defense response, as well as a disruption in kernel development.

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“…We previously constructed a genetic linkage map by using single nucleotide polymorphism (SNP) markers, generated from genotyping by sequencing (GBS) technology [Marocco, unpublished data]. Markers were developed on the segregating population derived from the cross between CO441 and CO354 maize lines, previously classified as resistant and susceptible to Fusarium ear rot [11, 16, 17]. Progress in breeding for resistance will be speeded up by analysis of new and consistent QTLs for Fusarium ear rot resistance and fumonisin accumulation and by a deeper knowledge of genetic mechanisms underlying maize- F. verticillioides interactions.…”

Section: Introductionmentioning

confidence: 99%

“…Microarray analysis was previously applied to characterize F. verticillioides -maize interaction [16, 17]. We reported that defense responses involved changes in the expression of a large number of genes in seeds of susceptible (CO354) and resistant (CO441) maize genotypes.…”

Section: Introductionmentioning

confidence: 99%

Functional genomic analysis of constitutive and inducible defense responses to Fusarium verticillioides infection in maize genotypes with contrasting ear rot resistance

et al. 2014

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BackgroundFusarium verticillioides causes ear rot in maize (Zea mays L.) and accumulation of mycotoxins, that affect human and animal health. Currently, chemical and agronomic measures to control Fusarium ear rot are not very effective and selection of more resistant genotypes is a desirable strategy to reduce contaminations. A deeper knowledge of molecular events and genetic basis underlying Fusarium ear rot is necessary to speed up progress in breeding for resistance.ResultsA next-generation RNA-sequencing approach was used for the first time to study transcriptional changes associated with F. verticillioides inoculation in resistant CO441 and susceptible CO354 maize genotypes at 72 hours post inoculation. More than 100 million sequence reads were generated for inoculated and uninoculated control plants and analyzed to measure gene expression levels. Comparison of expression levels between inoculated vs. uninoculated and resistant vs. susceptible transcriptomes revealed a total number of 6,951 differentially expressed genes. Differences in basal gene expression were observed in the uninoculated samples. CO441 genotype showed a higher level of expression of genes distributed over all functional classes, in particular those related to secondary metabolism category. After F. verticillioides inoculation, a similar response was observed in both genotypes, although the magnitude of induction was much greater in the resistant genotype. This response included higher activation of genes involved in pathogen perception, signaling and defense, including WRKY transcription factors and jasmonate/ethylene mediated defense responses. Interestingly, strong differences in expression between the two genotypes were observed in secondary metabolism category: pathways related to shikimate, lignin, flavonoid and terpenoid biosynthesis were strongly represented and induced in the CO441 genotype, indicating that selection to enhance these traits is an additional strategy for improving resistance against F. verticillioides infection.ConclusionsThe work demonstrates that the global transcriptional analysis provided an exhaustive view of genes involved in pathogen recognition and signaling, and controlling activities of different TFs, phytohormones and secondary metabolites, that contribute to host resistance against F. verticillioides. This work provides an important source of markers for development of disease resistance maize genotypes and may have relevance to study other pathosystems involving mycotoxin-producing fungi.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-710) contains supplementary material, which is available to authorized users.

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Differential gene expression in kernels and silks of maize lines with contrasting levels of ear rot resistance after Fusarium verticillioides infection

Cited by 66 publications

References 40 publications

“Omics” of Maize Stress Response for Sustainable Food Production: Opportunities and Challenges

“Omics” of Maize Stress Response for Sustainable Food Production: Opportunities and Challenges

Aspergillus flavus infection induces transcriptional and physical changes in developing maize kernels

Functional genomic analysis of constitutive and inducible defense responses to Fusarium verticillioides infection in maize genotypes with contrasting ear rot resistance

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