Differential gene expression in embryogenic and non-embryogenic clusters from cell suspension cultures ofCoffea arabica

Quiroz-Figueroa, Francisco Roberto; Méndez-Zeel, Marcela; Teyer, Lorenzo Felipe Sánchez; Rojas-Herrera, Rafael; Loyola‐Vargas, Víctor M.

doi:10.1078/0176-1617-00878

Cited by 34 publications

(21 citation statements)

References 20 publications

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“…Likewise, embryogenic and non-embryogenic tissues from Coffea arabica calli are discernible based on their color, with the former being brown and hard while the latter are pale and friable. This correlation between callus appearance and embryogenic competence also has been confirmed by histological studies (Quiroz-Figueroa et al, 2002).…”

supporting

confidence: 55%

Comparative anatomy of embryogenic and non-embryogenic calli frompimpinella brachycarpa

Kim

Kwack

et al. 2007

J. Plant Biol.

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Anatomical differences between embryogenic and non-embryogenic calli of Pimpinella brachycarpa were investigated by light microscopy and electron microscopy. Initial callus tissue emerged from explants after 14 d of culturing. The embryogenic calli (EC) were firm, rather opaque, and light yellow in color. The cells usually formed small, compact clusters. Nonembryogenic calli (NEC), however, were friable, semitransparent, and yellow or gray. These formed relatively larger and loosely held clusters. Scanning electron microscopy showed that EC were composed of individual compact and spherical cells that were rather regular in size and approximately 20 ~tm long. All were tightly held together and appeared to organize globular embryos. In contrast, the NEC comprised elongated and loosely held cells that were approximately 50 ~m long. Tubular and u-shaped NEC cells protruded irregularly, and were of varying heights along the cell aggregates. Transmission electron microscopy of the EC revealed typical eukaryotic cytoplasmic components, including nuclei, mitochondria, and vacuoles in the cytoplasm enclosed by an electron-transparent cell wall. Based on the numerous ribosomes within the cytoplasm, these cells appeared to be well-organized and metabolically active. The NEC cells were much larger and more highly vacuolated than those of the EC. In ultrathin sections, the former seemed to be almost devoid of other cellular contents except for plastids and nuclei. Furthermore, EC and NEC showed different regeneration capacities in their somatic embryo formation. Most EC produced hyperhydric somatic embryos, followed by normal somatic embryos; whereas only a few shooted or rooted somatic embryos arose from the NEC.

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supporting

confidence: 55%

Comparative anatomy of embryogenic and non-embryogenic calli frompimpinella brachycarpa

Kim

Kwack

et al. 2007

J. Plant Biol.

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“…In our own experience, embryogenic and non-embryogenic tissues from Coffea arabica calluses are discernible based on coloration; embryogenic calluses are brown (phenolized) and hard, while non-embryogenic are pale and friable ( Fig. 1); moreover, this correlation between calluses appearance and embryogenic competence has been confirmed by histological studies (Quiroz-Figueroa et al 2002b).…”

Section: Morphology Versus Embryogenic Capacitymentioning

confidence: 74%

Embryo production through somatic embryogenesis can be used to study cell differentiation in plants

Quiroz-Figueroa

Rojas-Herrera

Galáz-Ávalos

et al. 2006

Plant Cell Tiss Organ Cult

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221

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Somatic embryogenesis is the process by which somatic cells, under induction conditions, generate embryogenic cells, which go through a series of morphological and biochemical changes that result in the formation of a somatic embryo. Somatic embryogenesis differs from zygotic embryogenesis in that it is observable, its various culture conditions can be controlled, and a lack of material is not a limiting factor for experimentation. These characteristics have converted somatic embryogenesis into a model system for the study of morphological, physiological, molecular and biochemical events occurring during the onset and development of embryogenesis in higher plants; it also has potential biotechnological applications. The focus of this review is on embryo development through somatic embryogenesis and especially the factors affecting cell and embryo differentiation.

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“…The development of a globular structure in somatic embryogenesis is coupled to the concomitant development of the protodermis, the outermost layer of a developing embryo with evident anticlinal divisions and the presence of this layer is one of the characteristic features of somatic embryo development (Yeung 1995;von Arnold et al 2002). It has been proposed that the protodermis regulates further embryo development by applying physical and cell divisional limitations (Quiroz-Figueroa et al 2002). Interestingly, the protodermis was clearly visible in histological section at initial stages of somatic embryogenesis of saffron indicating that the emerging meristemoids were following the normal path of somatic embryos.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system

Blázquez¹,

Olmos

Hernández

et al. 2009

Plant Cell Tiss Organ Cult

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The ontogenetic developmental stages of saffron somatic embryogenesis have been studied and characterized using light microscopy and the biochemical determination of the antioxidant enzymatic system. The embryogenic callus underwent internal segmented divisions with the formation of globular embryos that were attached to the callus surface by a broad multicellular structure. Further development of the embryoids was characterized by the emergence of a shoot apical meristem and cotyledon (monopolar stage) with the subsequent differentiation of a minicorm at the basal part of the somatic embryo (dipolar stage). During the morphological differentiation of the somatic embryos changes in the antioxidant enzymatic system with increased superoxide dismutase (SOD) and catalase (CAT) activities were detected at the initial stages of somatic embryogenesis. The isoforms of SOD, including two Mn-SODs and four Cu, Zn-SODs, were also detected. Although all the isoforms were expressed during the successive stages of somatic embryogenesis, an increase in Mn-SODs and a decrease in Cu, Zn-SODs during the last two stages was observed. Significant changes were also detected in the antioxidant activities ascorbate peroxidase, dehydroascorbic acid reductase and glutathione reductase.

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Differential gene expression in embryogenic and non-embryogenic clusters from cell suspension cultures ofCoffea arabica

Cited by 34 publications

References 20 publications

Comparative anatomy of embryogenic and non-embryogenic calli frompimpinella brachycarpa

Comparative anatomy of embryogenic and non-embryogenic calli frompimpinella brachycarpa

Embryo production through somatic embryogenesis can be used to study cell differentiation in plants

Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system

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