Differential gene expression in cumulus oocyte complexes collected by ovum pick up from repeat breeder and normally fertile Holstein Friesian heifers

Puglisi, Roberto; Cambuli, Caterina; Capoferri, Rossana; Giannino, Laura; Lukaj, A.; Duchi, Roberto; Lazzari, Giovanna; Galli, Cesare; Feligini, Maria; Galli, Andrea; Bongioni, G.

doi:10.1016/j.anireprosci.2013.07.003

Cited by 16 publications

(12 citation statements)

References 51 publications

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“…Paczkowski et al [30] and Romar et al [35] reported significantly more transcripts of genes engaged in cell protection against oxidative stress (reductase and glutathione peroxidase) in more competent porcine oocytes. On the other hand, Puglisi [36] postulated that increased RA of the GSTA2 gene may be a response to cellular stress. Whether oocytes are being providently protected or subjected to stress conditions needs to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Whether oocytes are being providently protected or subjected to stress conditions needs to be elucidated. Considering the results of Romar [35] and Puglisi [36], increased GSTA2 expression in BCB+ oocytes may suggest a response to BCB test conditions. This hypothesis complements previous results showing that oocyte incubation for an additional 90 minutes in BCB solution exerted a negative effect on chromosome segregation [21].…”

Section: Discussionmentioning

confidence: 99%

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Differences in Cytoplasmic Maturation Between the BCB+ and Control Porcine Oocytes Do Not Justify Application of the BCB Test for a Standard IVM Protocol

Pawlak

Warzych

Chabowska

et al. 2014

Journal of Reproduction and Development

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The Brilliant Cresyl Blue (BCB) test relies on G6PDH activity and a simple protocol for the selection of higher quality oocytes. Although the BCB+ oocytes of all the species that have been investigated are characterized by superior quality when compared to BCB- counterparts, application of the test for embryo production still remains an open issue. The aim of our study was to compare BCB+ and the control oocytes (not subjected to the BCB test) in terms of selected aspects of cytoplasmic maturation (mtDNA copy number, mitochondria distribution, relative transcript abundance of six marker genes). The results of our study revealed more relevant differences within the BCB+ and the control oocytes (before and after IVM) than between the two categories of oocytes. There was no difference in the transcript abundance of the BCB+ and the control oocytes in 5 out of 6 analyzed genes (BMP15, GDF9, ATP5A1, EEF1A, ZAR1) and in mtDNA content (pre-IVM 179609 vs. 176595 and post-IVM 187243 vs. 246984, respectively). With regard to mitochondria distribution in pre- and post-IVM oocytes, there was nonsignificant tendency for a more frequent occurrence of the expected patterns in the BCB+ group. The results of the present study do not support the application of BCB staining in a routine IVM protocol due to relatively high similarity in selected parameters characterizing cytoplasmic maturation of BCB+ and control oocytes. This high similarity may results from the limited amount of less competent BCB- oocytes (10%) still present among nonselected oocytes of proper morphology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differences in Cytoplasmic Maturation Between the BCB+ and Control Porcine Oocytes Do Not Justify Application of the BCB Test for a Standard IVM Protocol

Pawlak

Warzych

Chabowska

et al. 2014

Journal of Reproduction and Development

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“…The MFGE8 (milk fat globule-EGF factor 8 protein), located in CSS-334, has been reported to be associated with an index assessing productivity and functional and conformation traits (Fontanesi et al, 2014 ), which may be relevant to the dual-purpose production characteristics of this breed. The same CSS also includes ISG20 (interferon stimulated exonuclease gene 20 kDa), which is involved in cumulus oocyte growth and may be related to fertility (Puglisi et al, 2013 ).…”

Section: Plausible Candidate Genes Underlying Css Regionsmentioning

confidence: 99%

An interpretive review of selective sweep studies in Bos taurus cattle populations: identification of unique and shared selection signals across breeds

2015

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This review compiles the results of 21 genomic studies of European Bos taurus breeds and thus provides a general picture of the selection signatures in taurine cattle identified by genome-wide selection-mapping scans. By performing a comprehensive summary of the results reported in the literature, we compiled a list of 1049 selection sweeps described across 37 cattle breeds (17 beef breeds, 14 dairy breeds, and 6 dual-purpose breeds), and four different beef-vs.-dairy comparisons, which we subsequently grouped into core selective sweep (CSS) regions, defined as consecutive signals within 1 Mb of each other. We defined a total of 409 CSSs across the 29 bovine autosomes, 232 (57%) of which were associated with a single-breed (Single-breed CSSs), 134 CSSs (33%) were associated with a limited number of breeds (Two-to-Four-breed CSSs) and 39 CSSs (9%) were associated with five or more breeds (Multi-breed CSSs). For each CSS, we performed a candidate gene survey that identified 291 genes within the CSS intervals (from the total list of 5183 BioMart-extracted genes) linked to dairy and meat production, stature, and coat color traits. A complementary functional enrichment analysis of the CSS positional candidates highlighted other genes related to pathways underlying behavior, immune response, and reproductive traits. The Single-breed CSSs revealed an over-representation of genes related to dairy and beef production, this was further supported by over-representation of production-related pathway terms in these regions based on a functional enrichment analysis. Overall, this review provides a comparative map of the selection sweeps reported in European cattle breeds and presents for the first time a characterization of the selection sweeps that are found in individual breeds. Based on their uniqueness, these breed-specific signals could be considered as “divergence signals,” which may be useful in characterizing and protecting livestock genetic diversity.

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“…This finding is interesting given the low heritability of traditional fertility traits, which are based on phenotype observation, and the recent trend toward an increasing use of genomic selection tools in dairy cattle breeding programs (25). The results of that study identified genes that are potential markers of fertility in dairy cattle, and those genes would prove useful when incorporated in genomic selection tools in dairy cattle breeding programs.…”

Section: Isg20 and Potential Biomarkersmentioning

confidence: 92%

“…Fertility in dairy cattle Microarray analysis and statistical validation of selected genes using qRT-PCR revealed that nine genes, including ISG20, were differentially expressed between repeat breeder (a normal estrous cycling animal that did not become pregnant after three inseminations despite the absence of clinically detectable reproductive disorders) and normally fertile Holstein Friesian heifers (25). This finding is interesting given the low heritability of traditional fertility traits, which are based on phenotype observation, and the recent trend toward an increasing use of genomic selection tools in dairy cattle breeding programs (25).…”

Section: Isg20 and Potential Biomarkersmentioning

confidence: 99%

Interferon-stimulated gene 20-kDa protein (ISG20) in infection and disease: Review and outlook

Zheng

Wang

Pan

2017

IRDR

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Differential gene expression in cumulus oocyte complexes collected by ovum pick up from repeat breeder and normally fertile Holstein Friesian heifers

Cited by 16 publications

References 51 publications

Differences in Cytoplasmic Maturation Between the BCB+ and Control Porcine Oocytes Do Not Justify Application of the BCB Test for a Standard IVM Protocol

Differences in Cytoplasmic Maturation Between the BCB+ and Control Porcine Oocytes Do Not Justify Application of the BCB Test for a Standard IVM Protocol

An interpretive review of selective sweep studies in Bos taurus cattle populations: identification of unique and shared selection signals across breeds

Interferon-stimulated gene 20-kDa protein (ISG20) in infection and disease: Review and outlook

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