Differential Gene Expression in B-Cell Non-Hodgkin's Lymphoma of SIV-Infected Monkey

Abstract

Infection with SIVmac251 in some rhesus monkeys (Macaca mulatta) leads to B-cell non-Hodgkin's lymphomas (B-NHL) clinically similar to that of HIV-infected AIDS patients. To further characterize the SIV-associated B-NHL we have generated genetic profiles of malignant cells by subtractive hybridization and Northern blot analysis. We have analyzed 21 clones of a subtracted cDNA library corresponding to overexpressed genes in diffuse large B-cell (DLBCL) SIV-associated monkey lymphoma. Eight of these clones represent a sequence homologous to an abundant transcript from KG-1 cells originally established from a human myelogenous leukemia. The protein encoded has a 60% similarity to a hypothetical glycine-rich transmembrane signal protein of Caenorhabditis elegans and 25% similarity to the ret finger protein. The other cDNA clones contained sequences of the serum amyloid A gene (SAA), the alpha1-acid glycoprotein gene (AGP), the ribosomal protein S3a (RPS3a) and L8 (RPL8) genes, the interferon-inducible gene (INF-ind), the metastasin gene (mts1), and the NADH dehydrogenase I gene (ND-I). The remaining cDNA clones consisted of yet unknown sequences. In addition, we detected an up-regulation of the cytochrome c oxidase II gene (COX-II), the ND-IV gene, and the SET oncogene by Northern blot hybridization in three SIV-associated NHLs of different histomorphological classification. All these genes have not previously been found to be overexpressed in B-NHL.

Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer

“…LDB1 is overexpressed in oral carcinoma (Mizunuma et al, 2003). Ribosomal proteins RPL8 (Tarantul et al, 2000), RPS13 (Denis et al, 1993), RPL5 (Frigerio et al, 1995), RPL23A (Jiang et al, 1997), RPL19 (Henry et al, 1993), RPL37 (Loging and Reisman, 1999), and RPS16 (Batra et al, 1991) are previously reported in association with multiple cancers other than NSCLC. Our data illustrate the success of our approach in discovering previously unidentified NSCLC-specific genes.…”

High Levels of TRIM14 Are Associated with Poor Prognosis in Hepatocellular Carcinoma

“…TRIM14 levels have been found to be upregulated in HIV-associated lymphomas in monkeys and in TSCC [4,5,10], suggesting a role of TRIM14 in cancer development and progression. However, the expression status of TRIM14 in HCC and its relationship with clinicopathological factors has not been identified.…”

“…TRIM14, previously known as KIAA0129, is an important member of the TRIM protein family. TRIM14 was first discovered in human immunodeficiency virus (HIV)-related human and simian non-Hodgkin's lymphomas [4,5]. Previous studies have shown that TRIM14 served as a mitochondrial adaptor that facilitates retinoic acidinducible gene-I-like receptor-mediated innate immune responses against viral infections [6].…”

The importance of mitochondria in the tumourigenic phenotype: Gliomas as the paradigm (Review)

“…Beheshti et al (19) found mtDNA amplification in neuroblastoma, and Haugen et al (20) found an increased expression of mtDNA-encoded genes in papillary thyroid carcinomas. Jia et al (21) showed increases in mtDNA levels with the mdr-1 genotype, and Tarantul et al (22) found evidence for amplified mitochondrial sequences in SIVinfected primate non-Hodgkin lymphoma (NHL). Similarly, Nikolaev et al (23) showed mtDNA increases in simian NHL, and Nenasheva et al (24) found that such sequences increased in SV40-transformed fibroblasts, observations suggesting that such modifications serve as early markers for the development and/or the pathogenesis of malignancy.…”