Differential Fragmentation of Mobility-Selected Glycans via Ultraviolet Photodissociation and Ion Mobility-Mass Spectrometry

Morrison, Kelsey A.; Clowers, Brian H.

doi:10.1007/s13361-017-1621-3

Cited by 28 publications

(35 citation statements)

References 26 publications

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“…Several recent studies showed that the integration of radical-induced dissociation with LC or IMS separation can be a powerful approach for characterization of glycan mixtures, including those containing structural isomers. 18–22…”

mentioning

confidence: 99%

De Novo Glycan Sequencing by Electronic Excitation Dissociation and Fixed-Charge Derivatization

Tang

Gao

et al. 2018

Anal. Chem.

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Detailed glycan structural characterization is frequently achieved by collisionally activated dissociation (CAD) based sequential tandem mass spectrometry (MS ) analysis of permethylated glycans. However, it is challenging to implement MS ( n > 2) during online glycan separation, and this has limited its application to analysis of complex glycan mixtures from biological samples. Further, permethylation can reduce liquid chromatographic (LC) resolution of isomeric glycans. Here, we studied the electronic excitation dissociation (EED) fragmentation behavior of native glycans with a reducing-end fixed charge tag and identified key spectral features that are useful for topology and linkage determination. We also developed a de novo glycan sequencing software that showed remarkable accuracy in glycan topology elucidation based on the EED spectra of fixed charge-derivatized glycans. The ability to obtain glycan structural details at the MS level, without permethylation, via a combination of fixed charge derivatization, EED, and de novo spectral interpretation, makes the present approach a promising tool for comprehensive and rapid characterization of glycan mixtures.

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confidence: 99%

De Novo Glycan Sequencing by Electronic Excitation Dissociation and Fixed-Charge Derivatization

Tang

Gao

et al. 2018

Anal. Chem.

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“…A third dimension can be added by perturbing the structure of native-like ions by increasing their internal energy. The most frequently used activation methods include collision-induced dissociation (CID) 34,35 , surface-induced dissociation (SID) 36,37 or ultraviolet photodissociation (UVPD) [38][39][40] . Other methods include electron capture dissociation (ECD) and electron transfer dissociation (ETD) 41 .…”

Section: Rd Dimension -Ion Reactivitymentioning

confidence: 99%

Software Requirements for the Analysis and Interpretation of Native Ion Mobility Mass Spectrometry Data

et al. 2020

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The last few years have seen a dramatic increase in applications of native mass and ion mobility spectrometry, especially for the study of proteins and protein complexes. This increase has been catalysed by the availability of commercial instrumentation capable of carrying out such analyses. Like in most fields, however, the software to process the data generated from new instrumentation lags behind. Recently, a number of research groups have started addressing this by developing software, but further improvements are still required in order to realise the full potential of the datasets generated. Here we describe practical aspects as well as challenges in processing native mass spectrometry (MS) and ion mobility-MS datasets, and provide a brief overview of currently available tools. We then set out our vision of future developments that would bring the community together and lead to the development of a common platform to expedite future computational developments, provide standardised processing approaches and serve as a location for the deposition of data for this emerging field.

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“…Moreover, they implemented synchrotron radiation laser producing extreme‐energy photons (10 nm < λ < 100 nm) to MS, to characterize the complex mixtures of oligosaccharides derived from red algae Porphyra umbilicalis . Also, to increase the UV photon adsorption to facilitate fragmentation, Morrison and Clowers used cobalt acetate as the adduct source .…”

Section: Advanced Dissociation Techniques For Glycomicsmentioning

confidence: 99%

Advances in mass spectrometry‐based glycomics

et al. 2018

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The diversification of the chemical properties and biological functions of proteins is attained through posttranslational modifications, such as glycosylation. Glycans, which are covalently attached to proteins, play a vital role in cell activities. The microheterogeneity and complexity of glycan structures associated with proteins make comprehensive glycomic analysis challenging. However, recent advancements in mass spectrometry (MS), separation techniques, and sample preparation methods have primarily facilitated structural elucidation and quantitation of glycans. This review focuses on describing recent advances in MS-based techniques used for glycomic analysis (2012-2018), including ionization, tandem MS, and separation techniques coupled with MS. Progress in glycomics workflow involving glycan release, purification, derivatization, and separation will also be highlighted here. Additionally, the recent development of quantitative glycomics through comparative and multiplex approaches will also be described.

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Differential Fragmentation of Mobility-Selected Glycans via Ultraviolet Photodissociation and Ion Mobility-Mass Spectrometry

Cited by 28 publications

References 26 publications

De Novo Glycan Sequencing by Electronic Excitation Dissociation and Fixed-Charge Derivatization

De Novo Glycan Sequencing by Electronic Excitation Dissociation and Fixed-Charge Derivatization

Software Requirements for the Analysis and Interpretation of Native Ion Mobility Mass Spectrometry Data

Advances in mass spectrometry‐based glycomics

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