Differential expressions of collagen types IV, III, and I during the development of invasive trophoblasts in rats

Abstract

We examined the differential expressions of collagen types IV, 111, and I in the developing feto-maternal placental tissue of pregnant rats by a combination of in situ hybridization and immunohistochemistry. At day 9.5 of gestation, polygonal invasive cytotrophoblasts from the ectoplacental cone, which was modifying the maternal central artery, revealed intensely expressed al(1V) and al(II1) collagen mRNAs. The localization patterns of these translated products, collagen type IV and procollagen type 111, were slightly different in the invasive cytotrophoblasts. Collagen type IV densely deposited intracellularly and intercellularly in the maternal central artery and in the thickened basement membranes of the cytotrophoblasts. However, expression of al(1) collagen mRNA and procollagen type I was hardly detectable in the cytotrophoblasts. At day 13 of gestation, a high level of al(1V) collagen mRNA was expressed in the cytotrophoblastic cell layer (trophospongium) and in the invasive large cytotrophoblasts. A moderate level of al(II1) collagen mRNA wag also expressed mainly in the cytotrophoblasts, while d(1) collagen mRNA was expressed at very low levels. Interestingly, procollagen type I11 failed to show linear immunoreactivity in the subepithelial extracellular matrix beneath the maternal artery with the invasive cytotrophoblasts. Additional quantitative analyses of these type IV, 111, and I collagen mRNA levels in in situ hybridization experiments between several cell types also revealed significant differences individually. Electron-microscopic study detected no cross-striated collagen fibers in the thickened basement membrane-like structures adjacent to the invasive cytotrophoblasts. Fibrillar and baaement membrane collagen gene expressions, their protein syntheses, and the processing of these procollagens seem to be developmentally regulated in the invasive cytotrophoblasts during the organization of feto-maternal placental tissue. The remodeling of the maternal central artery by the invasive cytotrophoblasts is important for ensur-0 1996 WILEY-LISS, INC.ing the adequate blood supply to the developing placenta and fetus. 6 1998 Wiley-Liss, Inc.

Uterine Spiral Artery Remodeling: The Role of Uterine Natural Killer Cells and Extravillous Trophoblasts in Normal and High‐Risk Human Pregnancies

“…Early human histological studies of uterine spiral arteries suggested that EVTs were the main contributors in arterial remodeling . More recent evidence demonstrates that the initiation of the remodeling process is carried out primarily by uNK cells, while the EVTs play more of a secondary role.…”

Double intratracheal instillation of keratinocyte growth factor prevents bleomycin-induced lung fibrosis in rats

“…[20][21][22][23] All these inserts were labelled with [ 35 S]deoxythymidine triphosphate using a conventional nick translation, 4,24,25 and specific radioactivities of these labelled probes were 2-3 10 8 cpm/ g DNA.…”

“…20,25 Affinity-purified anti-procollagen type I (Pro I) and anti-procollagen type III (Pro III) antibodies 20 and a polyclonal antibody against rat SP-A 26 were diluted 1:300, 1:100, and 1:300, respectively. Immunoreaction was performed using the Vectastain peroxidase rabbit ABC kit (Vector, Burlingame, CA, U.S.A.).…”