Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma

Kara, Murat; Yumrutaş, Önder; Özcan, Önder; Çelik, Özgür; Bozgeyik, Esra; Bozgeyik, İbrahim; Taşdemir, Şener

doi:10.1016/j.gene.2015.04.065

Cited by 134 publications

(109 citation statements)

References 45 publications

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“…Similar scenario is reported in case of miRNA. Thus, anti-onco miR-132 or -133b rather increased in tumor tissues (28)(29)(30). In addition, some reports showed that miR-34a, a representative anti-oncomir (31,32), was shown to be upregulated in cancer tissue rather than in normal mucosa (30,33).…”

Section: Discussionmentioning

confidence: 98%

Identification of microRNA-487b as a negative regulator of liver metastasis by regulation of KRAS in colorectal cancer

Hata

Mokutani

Takahashi

et al. 2016

International Journal of Oncology

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Abstract.Recent studies have shown that microRNAs (miRNAs) are involved in the progression of colorectal cancer (CRC). The aim of this study is to identify a novel miRNA that especially relates to liver metastasis and to explore the underlying mechanism. Differentially expressed miRNAs were analyzed using microarray, in primary CRC tumors without metastasis (n=16), those with liver metastasis (n=12), and liver metastatic lesions (n=8). We found that miR-487b level decreased in liver metastatic lesions, and qRT-PCR confirmed the results in the validating cohort (n=134). Survival analysis indicated that high expression of miR-487b was associated with better prognosis. In vitro studies were also performed to investigate the functional significance of miR-487b in human CRC cell lines. miR-487b showed an inhibitory effect on cell proliferation and invasion of CRC cells. miR-487b downregulated KRAS and inhibited its downstream signal pathways, and the luciferase reporter assay revealed that miR-487b directly targeted LRP6, a receptor for WNT/β-catenin signaling. These findings showed that decrease in miR-487b was related with liver metastasis. Our data suggest a possibility that miR-487b may suppress metastasis of CRC progression through inhibition of KRAS.

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Section: Discussionmentioning

confidence: 98%

Identification of microRNA-487b as a negative regulator of liver metastasis by regulation of KRAS in colorectal cancer

Hata

Mokutani

Takahashi

et al. 2016

International Journal of Oncology

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“…In a recent study using high-throughput qPCR method, the expression profile of some cancer-related genes and their regulatory miRNAs were identified in CRC patients, and miR-132 was found to be significantly deregulated in CRC [10]. Another previous study had reported that miR-132 could inhibit CRC cell invasion and epithelialmesenchymal transition (EMT) via targeting ZEB2, an EMT regulator [11].…”

Section: Cck-8 Assaymentioning

confidence: 99%

Decreased expression of miR-132 in CRC tissues and its inhibitory function on tumor progression

Chen

Han

et al. 2016

Open Life Sciences

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Objectives Colorectal cancer (CRC) is among the most common types of malignancies in the worldwide, and microRNAs (miRNAs) emerge as key regulators in carcinogenesis and tumor progression. Here we intended to address the expression and function of miR-132 in CRC cells. Methods Paired CRC tissues and several established cell lines were firstly collected. We performed qPCR to detect the expression of miR-132 in these tissues and cell lines. Cell proliferation and apoptosis were respectively monitored by CCK-8 assay and Annexin-V/PI staining followed by flow cytometry, after miR-132 was transiently overexpressed in RKO cells. Afterwards, Luciferase reporter assays were performed to examine the targeting of YAP1 by miR-132. Finally, qPCR and western blotting were also carried out to validate this targeting. Results MiR-132 was significantly decreased in CRC and its overexpression in RKO cells exerted tumor suppressing effects, including cell growth arrest and apoptosis promotion. Additionally, we proved that miR-132 could negatively regulate the expression of YAP1. Conclusion Our findings suggested that miR-132 was downregulated in CRC, and played as a tumor suppressor to inhibit cell proliferation and induce apoptosis. And these anti-tumor activities might be related with the targeting of YAP1 by miR-132.

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“…MiR-203a has been reported to be associated with various types of tumor, including esophageal squamous cell carcinoma (11), hepatocellular carcinoma (12), non-small cell lung cancer (13) and colorectal carcinoma (14). However, the function of miR-203a in GC remains unclear.…”

Section: Introductionmentioning

confidence: 99%

miR‑203a suppresses cell proliferation by targeting E2F transcription factor 3 in human gastric cancer

et al. 2017

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Abstract. MicroRNAs (miRs) are a class of short non-coding RNAs that serve an essential role in the tumorigenesis of gastric cancer (GC). MiR-203a has been reported as a tumor repressor in various types of human cancer. In the present study, the function of miR-203a on the proliferation of GC cells was investigated. Bioinformatics analyses revealed that miR-203a targets the 3'-untranslated region of E2F transcription factor 3 (E2F3) messenger RNA. A luciferase reporter assay and western blot analysis were performed to confirm whether E2F3 was a target of miR-203a. The relative luciferase activity was decreased when overexpressing miR-203a with E2F3-wild type pmirGLO-3'-untranslated region vector, compared with the control group in HEK293 cells. Overexpression of miR-203a suppressed cell proliferation and colony formation of SGC-7901 and AGS GC cells. Inhibition of miR-203a promoted the proliferation of GC cells. Collectively, the results indicated that miR-203a may function as a tumor suppressor in GC by targeting E2F3.

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Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma

Cited by 134 publications

References 45 publications

Identification of microRNA-487b as a negative regulator of liver metastasis by regulation of KRAS in colorectal cancer

Identification of microRNA-487b as a negative regulator of liver metastasis by regulation of KRAS in colorectal cancer

Decreased expression of miR-132 in CRC tissues and its inhibitory function on tumor progression

miR‑203a suppresses cell proliferation by targeting E2F transcription factor 3 in human gastric cancer

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