Differential expression profiling of estrogen receptor in the ovaries of two egg duck (Anas platyrhynchos) breeds

Abstract

AbstrAct:In the present study, quantitative real-time PCR was employed to investigate expression profiling and expression difference of ESR1 and ESR2 in ovaries of Shaoxing duck and Jingjiang duck during three laying stages. Results showed the expression levels of ESR1 and ESR2 in ovaries were increased from the age of the first egg to the age of 500 days in both duck breeds. The expression of ESR1 in Shaoxing duck was lower than that in Jingjiang duck for the age of the first egg and of 180 days, and for the age of 500 days it was higher in Shaoxing duck than in Jingjiang duck. The ESR2 showed converse expression profiling in the two duck breeds. The results suggest that ESR1 and ESR2 mediate the process of egg laying in ducks, and that ESR2 may play a more important role for the ovary during egg-laying stages and may be closely related to the laying performance of the ducks.

Effect of a Diet Supplemented with the Moringa oleifera Seed Powder on the Performance, Egg Quality, and Gene Expression in Japanese Laying Quail under Heat-Stress

“…Similarly, Riry et al [51] and Ahmad et al [38] reported the positive effects of M. oleifera seed meal on egg weight and egg production in laying quail and hens, respectively. Other studies have demonstrated the important role of ESR2 in the maintenance of ovarian function and effective reproduction, and a close relationship between ESR2 and laying performance was suggested [14,52]. Furthermore, Rangel et al [53] suggested that STAR plays a vital role in the circadian control of steroidogenesis in the F1 follicle and subsequently in the circadian timing of ovulation.…”

“…Heat stress directly affects ovarian function by altering folliculogenesis and the secretion of ovarian hormones, such as prolactin, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) [9]. Ovarian functions and folliculogenesis are mainly controlled by various genes, such as steroidogenic acute regulatory protein (STAR), inhibin subunit alpha (INHA), bone morphogenetic protein 15 (BMP-15), follicle-stimulating hormone receptor (FSHR), and estrogen receptors 1 and 2 (ESR1 and ESR2) [10][11][12][13][14].…”

Ovarian transcriptomic analysis and follicular development of Leizhou black duck

“…Other studies revealed that polymorphism in the PRL gene indicated PRL might be a candidate gene that influences egg-laying traits and reproduction in ducks ( Wang et al., 2011 ; Chuekwon and Boonlum, 2017 ; Bai et al., 2019 ). Estrogen receptor 2 has been studied to play vital roles in the ovary during egg-laying stages and may be closely related to laying performance in ducks and may regulate key genes in granulosa cells essential for follicle maturation and ovulation ( Wu et al., 2014 ; Khristi et al., 2018 ). Bone morphogenetic protein receptor beta is a member of the TGF-β superfamily which is firstly expressed in primordial follicles on the oocyte and granulosa cells of primary follicles throughout folliculogenesis ( Regan et al., 2018a ; Abdurahman et al., 2019 ; Zhang et al., 2019 ).…”

“…The steroid biosynthesis pathway is known as an important target for endocrine-disrupting chemicals that impair reproduction ( Sanderson, 2006 ). Ovarian steroid hormones such as estrogens ( ESR ) play an important signaling role in the female reproductive system and ensure normal follicular development by regulating the dynamic balance of follicular growth and development hormones ( Findlay et al., 2009 ; Vrtačnik et al., 2014 ; Wu et al., 2014 ; Khristi et al., 2018 ; Prasasya and Mayo, 2019 ). In this study, ESR 2 was significantly higher in HG than LG ducks which may contribute to higher egg-laying performance in HG ducks.…”

SNP analysis reveals estrogen receptor 1 (<i>ESR1</i>) gene variants associated with laying traits in quails

“…The role of ESR1 and ESR2 in the reproductive performance of poultry has been increasingly studied in recent years (Tang et al, 2009;Wang et al, 2012). ESR1 gene is one of the candidate genes for detecting polymorphisms associated with the reproductive and laying traits in pigs, chickens and other animals (Goliasova and Wolf, 2004;Humpolicek et al, 2007;Liu et al, 2010;Muñoz et al, 2007;Tang et al, 2009;Wang et al, 2012;Bi et al, 2005;Szreder and Zwierzchowski, 2007;Wu et al, 2014). The objectives of this study were to detect the polymorphism in five regions (exons 1, 2, 4, 8 and intron 1) of ESR gene in quail populations (the yellow-feather and chestnut-feather quails), which are bred in China and have relatively high laying performance and mature early (about 45 days), and to investigate its association with laying traits of two populations and further search the possibility of ESR1 gene being used as molecular genetic markers for laying traits.…”