Differential Expression Profile of MicroRNAs During Prolonged Storage of Platelet Concentrates As a Quality Measurement Tool in Blood Banks

Maués, Jersey Heitor da Silva; Moreira-Nunes, Caroline Aquino; Pontes, Thaís Brilhante; Vieira, Priscilla Cristina Moura; Montenegro, Raquel Carvalho; Lamarão, Letícia Martins; Lima, Eleonidas Moura; Burbano, Rommel Rodrı́guez

doi:10.1089/omi.2018.0126

Cited by 8 publications

(21 citation statements)

References 47 publications

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“…In addition, the occurrence of platelet apoptosis is characterized by the most common events such as cytoskeletal protein degradation, ATP depletion, caspase activation, and decreased mitochondrial membrane potential [12,17,18]. More recent studies revealed potential miRNAs as efficient biomarkers to identify the molecular mechanisms by which storage conditions for more than 5 days result in functional differences in human platelets stored in blood banks [7,19,20].…”

Section: Platelet Storage Lesionsmentioning

confidence: 99%

MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

Maués

Moreira-Nunes

Burbano

2019

Cells

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Background: Platelet concentrate (PC) is one of the main products used in a therapeutic transfusion. This blood component requires special storage at blood banks, however, even under good storage conditions, modifications or degradations may occur and are known as platelet storage lesions. Methods: This research was performed on scientific citation databases PubMed/Medline, ScienceDirect, and Web of Science, for publications containing platelet storage lesions. The results obtained mainly reveal the clinical applicability of miRNAs as biomarkers of storage injury and as useful tools for a problem affecting public and private health, the lack of PC bags in countries with few blood donors. The major studies listed in this review identified miRNAs associated with important platelet functions that are relevant in clinical practice as quality biomarkers of PC, such as miR-223, miR-126, miR-10a, miR-150, miR-16, miR-21, miR-326, miR-495, let-7b, let-7c, let-7e, miR-107, miR-10b, miR-145, miR-155, miR-17, miR-191, miR-197, miR-200b, miR-24, miR-331, miR-376. These miRNAs can be used in blood banks to identify platelet injury in PC bags. Conclusion: The studies described in this review relate the functions of miRNAs with molecular mechanisms that result in functional platelet differences, such as apoptosis. Thus, miRNA profiles can be used to measure the quality of storage PC for more than 5 days, identify bags with platelet injury, and distinguish those with functional platelets.

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Section: Platelet Storage Lesionsmentioning

confidence: 99%

MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

Maués

Moreira-Nunes

Burbano

2019

Cells

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“…These computational methodologies have resulted in more accurately identifying miRNAs located in different groups based on the days of storage [ 53 ]. In our previous study, we used these methodologies which identified changes in the expression profiles of 14 miRNAs that were associated with PSL [ 21 ]. In this current study, we confirmed that changes in the profiles of the new miRNAs correlated with the instability of the half-life of these transcripts on the fourth day, which coincided with the time of onset of PSL.…”

Section: Discussionmentioning

confidence: 99%

“…A limitation of this study is the relatively small number of miRNAs investigated, only 20 more expressed, among which, some have been intensively reviewed by the literature [ 7 , 30 , 64 , 65 , 66 ]. Our results also provided a panel of miRNAs that have had their functions elucidated in platelets such as miR-223 [ 24 , 66 ], miR-20a [ 23 ] miR-126 [ 67 ], miR-10a/miR-10b [ 68 ], miR-326 [ 69 ], miR-150/miR-501/miR-338-5p/miR-432-5p/miR-411-5p [ 21 ], miR-570 [ 18 ], miR-495 [ 70 ], and miR-21/miR-27b [ 71 ]. For example, specific cold storage conditions caused changes in miRNA expressions, increasing levels of miR-20a/miR-10a/miR-16-2/miR-223, which correlated with the quality of platelets under specific conditions of storage [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs (miRNAs) comprise a class of molecules with a biological role capable of acting together to mediate sequence-specific regulation by repressing or degrading mRNAs at specific non-translated binding sites [ 19 ] and are a relevant tool for understanding storage lesions, proven by studies on stored platelets that continue to translate mRNA proteins [ 20 , 21 , 22 , 23 ]. Platelets contain an abundance of RNAs, miRNAs and have fully functional mRNA splicing machinery.…”

Section: Introductionmentioning

confidence: 99%

“…We found that the inverse expression relationship between miR-127 and miR-320a allowed us to identify PC bags that could still have physiologically normal (non-activated) platelets [ 20 ]. In addition, we identified another 14 miRNAs expressed differentially, comparing a control PC from the first day of storage with the PCs on each of the subsequent five days of storage from day one to day seven [ 21 ]. In this study, we used a new approach with bioinformatics and computational biology methods to identify and characterize the profile of new miRNAs and their sequence variants in platelet concentrates stored for seven days in a blood bank.…”

Section: Introductionmentioning

confidence: 99%

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Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank

2020

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Platelet concentrate (PC) transfusions are widely used to save the lives of patients who experience acute blood loss. MicroRNAs (miRNAs) comprise a class of molecules with a biological role which is relevant to the understanding of storage lesions in blood banks. We used a new approach to identify miRNAs in normal human platelet sRNA-Seq data from the GSE61856 repository. We identified a comprehensive miRNA expression profile, where we detected 20 of these transcripts potentially expressed in PCs stored for seven days, which had their expression levels analyzed with simulations of computational biology. Our results identified a new collection of miRNAs (miR-486-5p, miR-92a-3p, miR-103a-3p, miR-151a-3p, miR-181a-5p, and miR-221-3p) that showed a sensitivity expression pattern due to biological platelet changes during storage, confirmed by additional quantitative real-time polymerase chain reaction (qPCR) validation on 100 PC units from 500 healthy donors. We also identified that these miRNAs could transfer regulatory information on platelets, such as members of the let-7 family, by regulating the YOD1 gene, which is a deubiquitinating enzyme highly expressed in platelet hyperactivity. Our results also showed that the target genes of these miRNAs play important roles in signaling pathways, cell cycle, stress response, platelet activation and cancer. In summary, the miRNAs described in this study, have a promising application in transfusion medicine as potential biomarkers to also measure the quality and viability of the PC during storage in blood banks.

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Role of MicroRNA-326 and its Target Genes Bcl-xL and Bak as Potential Markers in Platelet Storage Lesion in Blood Banks

Elgendy

Swelem

Aboudiba

et al. 2022

Indian J Hematol Blood Transfus

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Platelet transfusion is crucial in the management of various conditions such as quantitative and qualitative platelet disorders. A serious problem that impacts public health is the shortage of Platelet concentrates (PCs) that frequently affect few blood donors’ countries, such as Egypt. This has necessitated the need to establish novel standards for determining the quality of PC during storage. It was found that microRNAs (miRNA) differential expression profile is a helpful tool for recognition of physiological platelet changes during storage. The aim of the current study was to highlight the role of platelet miRNA-326 and its putative target apoptotic genes, Bcl-xL and Bak, and their role in platelet storage lesion (PSL). Differential expression of miRNA-326 and its target genes in the apoptotic pathway, Bcl-xL and Bak was done using quantitative real time PCR (QR-PCR) on different storage points at day 0, day 3 and day 5 in blood bank. The results of the current study revealed over expression of miRNA-326 throughout days of storage resulted in down regulation of Bcl-xL gene and subsequently up regulation of Bak gene. MiRNA-326 contributes to platelet apoptosis and PSL through inhibition of anti-apoptotic Bcl-xL expression and enhancing pro-apoptotic Bak expression. Differential miRNA-326 and its target gene, Bcl-xL and Bak, expression levels at different points of platelets storage are promising tools as biomarkers for platelets undergoing PSL in blood banks.

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Differential Expression Profile of MicroRNAs During Prolonged Storage of Platelet Concentrates As a Quality Measurement Tool in Blood Banks

Cited by 8 publications

References 47 publications

MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank

Role of MicroRNA-326 and its Target Genes Bcl-xL and Bak as Potential Markers in Platelet Storage Lesion in Blood Banks

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