Differential expression profile of innate immune genes in the liver of olive flounder (Paralichthys olivaceus) against viral haemorrhagic septicaemia virus (VHSV) at host susceptible and non-susceptible temperatures

Kole, Sajal; Avunje, Satheesha; Jung, Sung‐Ju

doi:10.1016/j.aquaculture.2018.12.085

Cited by 5 publications

(2 citation statements)

References 54 publications

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“…The viral peptides presented by MHC I are recognized by CD8 + T lymphocytes which proliferate and differentiate to become CTL that in turn recognize virus-infected cells and induce several cell death inducers of the apoptotic pathway (94). Earlier experiments in floundrs have revealed that the expression of CD8 was significantly repressed at 15°C, however, it showed an up-regulated expression at 20°C (95). Therefore, it is speculated that the decrease of b2M could reduce the synthesis of MHC I molecular complex, causing the decrease of CD8 + T lymphocytes proliferation.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Temperature on the Antiviral Response of mIgM+ B Lymphocytes Against Hirame Novirhabdovirus in Flounder (Paralichthys olivaceus)

Tang

Cao

et al. 2022

Front. Immunol.

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Hirame novirhabdovirus (HIRRV) is an ongoing threat to the aquaculture industry. The water temperature for the onset of HIRRV is below 15°C, the peak is about 10°C, but no mortality is observed over 20°C. Previous studies found the positive signal of matrix protein of HIRRV (HIRRV-M) was detected in the peripheral blood leukocytes of viral-infected flounder. Flow cytometry and indirect immunofluorescence assay showed that HIRRV-M was detected in mIgM+ B lymphocytes in viral-infected flounder maintained at 10°C and 20°C, and 22% mIgM+ B lymphocytes are infected at 10°C while 13% are infected at 20°C, indicating that HIRRV could invade into mIgM+ B lymphocytes. Absolute quantitative RT-PCR showed that the viral copies in mIgM+ B lymphocytes were significantly increased at 24 h post infection (hpi) both at 10°C and 20°C, but the viral copies in 10°C infection group were significantly higher than that in 20°C infection group at 72 hpi and 96 hpi. Furthermore, the B lymphocytes were sorted from HIRRV-infected flounder maintained at 10°C and 20°C for RNA-seq. The results showed that the differentially expression genes in mIgM+ B lymphocyte of healthy flounder at 10°C and 20°C were mainly enriched in metabolic pathways. Lipid metabolism and Amino acid metabolism were enhanced at 10°C, while Glucose metabolism was enhanced at 20°C. In contrast, HIRRV infection at 10°C induced the up-regulation of the Complement and coagulation cascades, FcγR-mediated phagocytosis, Platelets activation, Leukocyte transendothelial migration and Natural killer cell mediated cytotoxicity pathways at 72 hpi. HIRRV infection at 20°C induced the up-regulation of the Antigen processing and presentation pathway at 72 hpi. Subsequently, the temporal expression patterns of 16 genes involved in Antigen processing and presentation pathway were investigated by qRT-PCR, and results showed that the pathway was significantly activated by HIRRV infection at 20°C but inhibited at 10°C. In conclusion, HIRRV could invade into mIgM+ B lymphocytes and elicit differential immune response under 10°C and 20°C, which provide a deep insight into the antiviral response in mIgM+ B lymphocytes.

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Section: Discussionmentioning

confidence: 99%

The Influence of Temperature on the Antiviral Response of mIgM+ B Lymphocytes Against Hirame Novirhabdovirus in Flounder (Paralichthys olivaceus)

Tang

Cao

et al. 2022

Front. Immunol.

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“…The highest number of CD4-2 + T cells was observed in the liver at 7 dpi, which suggests direct involvement of virus-specific Th1 (CD4-2 + ) T cells during the peak of acute infection [ 40 ]. Although the liver-related immune response in teleost has not been investigated much yet, the hepatic immune of fish has recently been reporting including rainbow trout ( Oncorhynchus mykiss ), Arctic charr ( Salvelinus alpinus ), and olive flounder ( Paralichthys olivaceus ) [ 41 , 42 , 43 , 44 , 45 , 46 ]. Early infection with VHSV has been shown to increase transcription levels of some genes in the liver of rainbow trout, namely CD3 and CD4 [ 45 ], while CD4 and CD8 were seen to be increased in olive flounder [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)

et al. 2021

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Viral hemorrhagic septicemia (VHS), caused by viral hemorrhagic septicemia virus (VHSV), is a viral disease affecting teleosts, and is the major cause of virus-related deaths in olive flounder (Paralichthys olivaceus). Research has focused on ways to control VHS, and recently, the use of polyinosinic-polycytidylic acid poly (I:C)-potentiated vaccination has been investigated, whereby fish are injected with poly (I:C) and then with live pathogenic virus, resulting in a significant decrease in VHSV-related mortality. T cell responses were investigated in the present study after vaccinating olive flounder with poly (I:C)-potentiated vaccination to understand the ability of poly (I:C) to induce T cell immunity. Stimulation of T cell responses with the poly (I:C)-potentiated vaccination was confirmed by examining levels of CD3+ T cells, CD4-1+ T cells and CD4-2+ T cells. Higher levels of CD4-2+ T cells were found in vaccinated fish than CD4-1+ T cells, believed to result from a synergistic effect between poly (I:C) administration and pathogenic VHSV immunization. More importantly, the role of CD4-2+ T cells in the antiviral response was clearly evident. The results of this study suggest that the outstanding protection obtained with the poly (I:C)-potentiated vaccination is due to the robust immune response initiated by the CD4-2+ T cells.

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The persistence of viral hemorrhagic septicemia virus (VHSV) mRNA in juvenile olive flounder, Paralichthys olivaceus following live VHSV vaccination

Jang,

Kim,

Yoon

et al. 2023

Aquaculture Reports

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Differential expression profile of innate immune genes in the liver of olive flounder (Paralichthys olivaceus) against viral haemorrhagic septicaemia virus (VHSV) at host susceptible and non-susceptible temperatures

Cited by 5 publications

References 54 publications

The Influence of Temperature on the Antiviral Response of mIgM+ B Lymphocytes Against Hirame Novirhabdovirus in Flounder (Paralichthys olivaceus)

The Influence of Temperature on the Antiviral Response of mIgM+ B Lymphocytes Against Hirame Novirhabdovirus in Flounder (Paralichthys olivaceus)

Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)

The persistence of viral hemorrhagic septicemia virus (VHSV) mRNA in juvenile olive flounder, Paralichthys olivaceus following live VHSV vaccination

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