Differential expression patterns of the two paralogous Rec8 from Nile tilapia and their responsiveness to retinoic acid signaling

Luo, Yubing; Wang, Jie; Bai, Xu; Xiao, Hesheng; Zhou, Lei; Wang, Deshou; Wei, Jing

doi:10.1016/j.cbpb.2021.110563

Cited by 4 publications

(1 citation statement)

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“…Synaptonemal complex protein 3 (Scp3), encoded by scp3 , is a meiotic marker expressed in the nucleus of spermatocytes and is commonly used to trace the timing of gonadal differentiation in vertebrates [ 37 ]. Rec8 is a meiotic cohesion gene expressed in the testis but not in the kidney or liver; more precisely, it is expressed in spermatocytes and spermatids but not in spermatogonia or other somatic cells in vertebrates [ 38 , 39 ]. Under normal culture conditions, a low level of mRNAs of both genes was present in SG3 cells.…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Coilia nasus Gonadal Somatic Cell Line Capable of Sperm Induction In Vitro

Kan

Zhang

Jawad

et al. 2022

Biology

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Coilia nasus is an important economic anadromous migratory fish of the Yangtze River in China. In recent years, overfishing and the deterioration of the ecological environment almost led to the extinction of the wild resources of C.nasus. Thus, there is an urgent need to protect this endangered fish. Recently, cell lines derived from fish have proven a promising tool for studying important aspects of aquaculture. In this study, a stable C. nasus gonadal somatic cell line (CnCSC) was established and characterized. After over one year of cell culture (>80 passages), this cell line kept stable growth. RT-PCR results revealed that the CnGSC expressed some somatic cell markers such as clu, fshr, hsd3β, and sox9b instead of germ cell markers like dazl, piwi, and vasa. The strong phagocytic activity of CnGSC suggested that it contained a large number of Sertoli cells. Interestingly, CnGSC could induce medaka spermatogonial cells (SG3) to differentiate into elongated spermatids while co-cultured together. In conclusion, we established a C. nasus gonadal somatic cell line capable of sperm induction in vitro. This research provides scientific evidence for the long-term culture of a gonadal cell line from farmed fish, which would lay the foundation for exploring the regulatory mechanisms between germ cells and somatic cells in fish.

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Section: Resultsmentioning

confidence: 99%