Differential expression patterns of gangliosides in the tissues and cells of NIH-mini pig kidneys

Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2

“…However, our findings go beyond that by showing direct binding interactions between the RBD and gangliosides, that inhibition of UGCG has no effect in cells lacking Sia and that genetic ablation of UGCG also causes a decrease in viral infection by both pseudotyped and authentic virus. It is worth noting that HEK293 cells, which we used extensively in our experiments, contain predominantly GM3, GM2, GD3 and GT1b along with trace amounts of GM1 49 .…”

“…A high-energy C-trap dissociation (HCD) energy of 50 V (using nitrogen as collision gas) with an isolation window of m/z 3,000-4,000 was used for screening against RBD; an HCD energy of 300 V (using argon) with an isolation window of m/z 8,000-15,000 was used for screening against S protein. The different classes of oligosaccharides are distinguished by color: red, Lewis antigens (1-14); yellow, blood group A antigens(15)(16)(17)(18)(19)(20); orange, blood group B antigens(21)(22)(23)(24)(25)(26); green, blood group H antigens(27)(28)(29)(30)(31)(32); blue, sulfated compounds(34)(35)(36)(37)(38) and HS oligosaccharides(39)(40)(41); light blue, antigen-related glycans(42)(43)(44)(45)(46)(47)(48)(49)(50); purple, globo (51-60); pink, ganglioside oligosaccharides (61-76); white, HMOs and other glycans (77-128); light gray, Neu5Acα2-6-linked oligosaccharides (129-132); dark gray, rhamnose-containing compounds (133-139); NA, not applicable. An asterisk indicates that the relative abundances (in CaR-ESI-MS) were estimated from their relative (to 69) K d .…”

“…Measurements were performed in negative ion mode with a UHMR Orbitrap mass spectrometer at an HCD energy of 50 V. Aqueous ammonium acetate (100 mM, pH 6.9) solutions of SARS-CoV-2 RBD (10 μM) and glycan library (containing 50 nM of each glycan) at were used for screening. The different classes of oligosaccharides are distinguished by colour: red -Lewis antigens (1-14); yellow -blood group A antigens(15)(16)(17)(18)(19)(20); orange -blood group B antigens(21)(22)(23)(24)(25)(26); green -blood group H antigens(27)(28)(29)(30)(31)(32); blue -sulfated compounds(34)(35)(36)(37)(38) and HS oligosaccharides(39)(40)(41), light blue -antigen-related glycans(42)(43)(44)(45)(46)(47)(48)(49)(50), purple -globo (51-60), pink -ganglioside oligosaccharides (61-76), white -HMOs and other glycans (77-128), light grey -Neu5Acα2-6-linked oligosaccharides (129-132), and dark grey -rhamnose-containing compounds (133-139). NA, not applicable.…”

Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells

“…Gangliosides are ubiquitous components of the membranes of mammalian cells, and are thought to have roles in cell proliferation, adhesion, migration, differentiation, survival, and immunosuppressive activity [10,11,21-31] however, their role in the immune rejection response to xenotransplantation is not understood.…”