Differential Expression of Wnt Genes in Normal and Flat Variants of PC 12 Cells, a Cell Line Responsive to Ectopic Wnt1 Expression

Erdreich‐Epstein, Anat; Shackleford, Gregory M.

doi:10.3109/08977199809117190

Cited by 9 publications

(10 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although 10 isoforms of Fz receptors have been identified in mammals, their binding specificities with Wnt and functions are poorly understood. Here, we demonstrated that PC12 cells express most of the Fz receptors, except Fz 8, 9, and 10, which is in accordance with a previous study in which Wnt signaling was observed in PC12 cells (25). Fz receptors seem to have a functional role in PC12 cells since their activation by Wnt-3a recombinant protein induced an increase in β-catenin expression.…”

Section: Discussionsupporting

confidence: 92%

Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults

Kawamoto

Gleichmann

Yshii

et al. 2012

Braz J Med Biol Res

View full text Add to dashboard Cite

Wnt proteins are involved in tissue development and their signaling pathways play an important role during embryogenesis. Wnt signaling can promote cell survival, which is beneficial for neurons, but could also lead to tumor development in different tissues. The present study investigated the effects of a Wnt protein on the susceptibility of a neural tumor cell line (PC12 cells) to the cytotoxic compounds ferrous sulfate (10 mM), staurosporine (100 and 500 nM), 3-nitropropionic acid (5 mM), and amyloid β-peptide (Aβ25-35; 50 µM). Cells (1 × 106 cells/mL) were treated with the Wnt-3a recombinant peptide (200 ng/mL) for 24 h before exposure to toxic insults. The Wnt-3a protein partially protected PC12 cells, with a 6-15% increase in cell viability in the presence of toxic agents, similar to the effect measured using the MTT and lactate dehydrogenase cell viability assays. The Wnt-3a protein increased protein expression of β-catenin by 52% compared to control. These findings suggest that Wnt signaling can protect neural cells against apoptosis induced by toxic agents, which are relevant to the pathogenesis of Alzheimer's and Huntington's diseases.

show abstract

Section: Discussionsupporting

confidence: 92%

Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults

Kawamoto

Gleichmann

Yshii

et al. 2012

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…For example, both PC12 derivatives exhibit an up-regulation of FZ2 expression and down-regulation of FZ5 expression . Furthermore, unlike wild type cells, they both express Wnt-7b and the GLAST glutamate transporter, and they exhibit transporter-mediated glutamate uptake (Ramachandran et al, 1993;Edreich-Epstein and Shackleford, 1998). We report that Wnt-3a-expressing PC12 cells (PC12/Wnt-3a) are like PC12/Wnt-1 in that NGF-induced neurite outgrowth is inhibited but differ in not exhibiting a flattened morphology, transporter-mediated glutamate uptake, or increased proliferation rate.…”

Section: Introductionmentioning

confidence: 91%

Inhibition by Wnt-1 or Wnt-3a of nerve growth factor-induced differentiation of PC12 cells is reversed by bisindolylmaleimide-I but not by several other PKC inhibitors

Chou

Howard

2002

Oncogene

View full text Add to dashboard Cite

Wnt-1 and Wnt-3a exhibit redundancy in neural crest development. We have found that they do not produce the same effects on PC12 cells, which were obtained from the adrenal medulla, a neural crest derivative. However, both Wnt-1 or Wnt-3a inhibit nerve growth factor (NGF)-induced neurite outgrowth. The inhibition is reversed by the protein kinase C (PKC) inhibitor, bisindolylmaleimide-I, but it did not reverse Wnt-1-induced activation of the canonical Wnt pathway. The Wnt-1 inhibitory effect was not reversed by several other PKC inhibitors, by phorbol ester-induced down-regulation of PKC, or by pertussis toxin, which is known to inhibit another Wnt signaling pathway, the Wnt/Ca 2+ pathway. We suggest that bisindolylmaleimide-I acts by affecting either a pathway downstream from Lef-1/Tcf in the canonical pathway or a Wnt signaling pathway other than the canonical pathway. In either case, the bisindolylmaleimide-I sensitivity of this pathway should aid in its identification.

show abstract

“…Cells-PC12 cells are widely employed as a neuronal cell model, are known to activate the canonical WNT pathway in response to WNT1 (39 -44), and do not express WNT7A (45). We therefore selected this cell model to assay the capacity of WNT7A to activate the canonical WNT pathway.…”

Section: Wnt7a Signals Via the Canonical Pathway In Pc12mentioning

confidence: 99%

“…PC12 cells do not express WNT7A mRNA (see Ref. 45) and are therefore particularly suitable for the study of WNT7A responses. Because WNT proteins associate with glycosaminoglycans in the extracellular matrix and are tightly bound to the cell surface, it is often difficult to collect WNT from the culture medium (reviewed in Ref.…”

Section: A Fzd5⅐lrp6 Complex Can Function As a Wnt7a Receptor-twomentioning

confidence: 99%

Functional Characterization of WNT7A Signaling in PC12 Cells

Caricasole

Ferraro

Iacovelli

et al. 2003

Journal of Biological Chemistry

127

View full text Add to dashboard Cite

WNT factors represent key mediators of many processes in animal development and homeostasis and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). In mammals, 19 genes encoding Wingless and Int-related factor (WNTs), 10 encoding Frizzled, and 2 encoding LRP proteins have been identified, but little is known of the identities of individual Frizzled-LRP combinations mediating the effects of specific WNT factors. Additionally, several secreted modulators of WNT signaling have been identified, including at least three members of the Dickkopf family. WNT7A is a WNT family member expressed in the vertebrate central nervous system capable of modulating aspects of neuronal plasticity. Gene knock-out models in the mouse have revealed that WNT7A plays a role in cerebellar maturation, although its function in the development of distal limb structures and of the reproductive tract have been more intensely studied. To identify a receptor complex for this WNT family member, we have analyzed the response of the rat pheochromocytoma cell line PC12 to WNT7A. We find that PC12 cells are capable of responding to WNT7A as measured by increased ␤-catenin stability and activation of a T-cell factor-based luciferase reporter construct and that these cells express three members of the Frizzled family (Frizzled-2, -5, and -7) and LRP6. Our functional analysis indicates that WNT7A can specifically act via a Frizzled-5⅐LRP6 receptor complex in PC12 cells and that this activity can be antagonized by Dickkopf-1 and Dickkopf-3.Members of the WNT gene family encode structurally related secreted glycoprotein factors, modulating a vast array of processes during vertebrate and invertebrate embryonic development as well as several aspects of tissue homeostasis in the adult (1-4). In embryos, signaling by WNT factors controls the organization of the body plan during the early stages of development as well as organogenesis at later developmental stages. Postnatally, WNT signaling is involved in normal biological events such as tissue maturation and homeostasis and in several neoplastic pathologies (2, 5-8). For example, in the mammalian central nervous system (CNS) 1 WNT signal transduction is involved in neural induction and patterning in early embryogenesis (2, 4) as well as in organogenesis and neuronal homeostasis at later stages (9). In the adult, WNTs play a role in the control of neuronal plasticity and are implicated in CNS neoplasias such as medulloblastoma (10 -14). The analysis of the signaling events mediated by WNTs has uncovered at least three signal transduction pathways, each involved in the mediation of specific biological responses (2, 15). The most studied and best understood signaling cascade elicited by WNTs involves an interaction with a receptor complex comprising members of the Frizzled (FZD) class of 7-transmembrane receptors and a member of the low density lipoprotein receptor (LRP) family of single-pass membrane proteins (16). WNT interaction with its r...

show abstract

Differential Expression of Wnt Genes in Normal and Flat Variants of PC 12 Cells, a Cell Line Responsive to Ectopic Wnt1 Expression

Cited by 9 publications

References 51 publications

Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults

Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults

Inhibition by Wnt-1 or Wnt-3a of nerve growth factor-induced differentiation of PC12 cells is reversed by bisindolylmaleimide-I but not by several other PKC inhibitors

Functional Characterization of WNT7A Signaling in PC12 Cells

Contact Info

Product

Resources

About