Differential Expression of Three Distinct Potassium Currents in the  Ventral Cochlear Nucleus

Rothman, Jason S.; Manis, Paul B.

doi:10.1152/jn.00125.2002

Cited by 104 publications

(130 citation statements)

References 65 publications

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“…To isolate I h , recordings were made in the presence of 2 mM 4-AP. Figure 2B shows that 2 mM 4-AP reduced I inst , perhaps as a result of blocking a small, lowvoltage activated K ϩ current (I KL ) (Rothman and Manis 2003). FIG. 1.…”

Section: Isolation and Estimation Of I Hmentioning

confidence: 94%

“…Of the resting conductances, ϳ15 nS in both types of stellate cells, ϳ20% is blocked by 2 mM 4-AP. 4-AP blocks low-voltage-activated K ϩ conductances (Bal and Oertel 2001;Banks et al 1993;Brew and Forsythe 1995;Manis and Marx 1991;Rathouz and Trussell 1998;Rothman and Manis 2003;Wu 1999) and a small proportion of g h (Bal and Oertel 2000;present experiments). The present experiments show that the voltage-sensitive g h accounts for ϳ10% of the input conductance and that the voltage-insensitive, ZD7288-sensitive conductance contributes roughly another 20%.…”

Section: Factors That Determine the Excitability Of T And D Stellate mentioning

confidence: 98%

“…R a was partially compensated on-line between 60 and 75% with a 20-s lag time. For analysis of the voltage dependence of activation, the voltage drop caused by the uncompensated R a was subtracted off-line from the applied voltage command to yield a precise value of transmembrane voltage (Rothman and Manis 2003). Voltage responses to current injection and current responses to voltage steps were recorded at 40 and 5 kHz and filtered at 10 and 1 kHz, respectively.…”

Section: Electrophysiological Recordingsmentioning

confidence: 99%

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Hyperpolarization-Activated Currents Regulate Excitability in Stellate Cells of the Mammalian Ventral Cochlear Nucleus

Rodrigues¹,

Oertel²

2006

Journal of Neurophysiology

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Rodrigues, Aldo Rogelis A. and Donata Oertel. Hyperpolarizationactivated currents regulate excitability in stellate cells of the mammalian ventral cochlear nucleus. J Neurophysiol 95: 76 -87, 2006. First published September 28, 2005 doi:10.1152/jn.00624.2005. The differing biophysical properties of neurons the axons of which form the different pathways from the ventral cochlear nucleus (VCN) determine what acoustic information they can convey. T stellate cells, excitatory neurons the axons of which project locally and to the inferior colliculus, and D stellate cells, inhibitory neurons the axons of which project to the ipsi-and contralateral cochlear nuclei, fire tonically when they are depolarized, and, unlike other cell types in the VCN, their firing rates are sensitive to small changes in resting currents. In both types of neurons, the hyperpolarization-activated current (I h ) reversed at -40 mV, was activated at voltages negative to Ϫ60 mV, and half-activated at approximately Ϫ88 mV; maximum hyperpolarization-activated conductances (g h max ) were 19.1 Ϯ 2.3 nS in T and 30.3 Ϯ 2.6 nS in D stellate cells (means Ϯ SE). Activation and deactivation were slower in T than in D stellate cells. In both types of stellate cells, 50 M 4(N-ethyl-N-phenylamino)1,2-dimethyl-6-(methylamino) pyridinium chloride (ZD7288) and 2 mM Cs ϩ blocked a 6-to 10-fold greater conductance than the voltage-dependent g h determined from Boltzmann analyses at -62 mV. The voltageinsensitive, ZD7288-sensitive conductance was proportional to g h max and g input . 8-Br-cAMP shifted the voltage dependence of I h in the depolarizing direction, increased the rate of activation, and slowed its deactivation in both T and D stellate cells. Reduction in temperature did not change the voltage dependence but reduced the maximal g h with a Q 10 of 1.3 and slowed the kinetics with a Q 10 of 3.3.

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Section: Isolation and Estimation Of I Hmentioning

confidence: 94%

Section: Factors That Determine the Excitability Of T And D Stellate mentioning

confidence: 98%

Section: Electrophysiological Recordingsmentioning

confidence: 99%

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Hyperpolarization-Activated Currents Regulate Excitability in Stellate Cells of the Mammalian Ventral Cochlear Nucleus

Rodrigues¹,

Oertel²

2006

Journal of Neurophysiology

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“…Our results on the temperature dependence of the spiking threshold reveal that, for the EPSC stimulus with a given frequency, there is a temperature sensitive range for the neuron, and that this range reduces as the stimulus frequency increases. These imply that there is a physiological temperature range which is beneficial to the information processing in auditory system.The model used in this work is presented by Rothman and Manis (RM) [10] for bushy cells in ventral cochlear nucleus of auditory midbrain based on electrophysiological experiments [19]. It consists of a single electrical compartment with a membrane capacitance (C) connected in parallel with a fast-activating slow-inactivating low-threshold K + current (I LT ), a high-threshold K + current (I HT ), a fast-inactivating TTX-sensitive Na + current (I N a ), a hyperpolarizationactivated cation current (I h ), a leakage current (I lk ), and an excitatory synaptic current (I E ).…”

mentioning

confidence: 99%

“…The model used in this work is presented by Rothman and Manis (RM) [10] for bushy cells in ventral cochlear nucleus of auditory midbrain based on electrophysiological experiments [19]. It consists of a single electrical compartment with a membrane capacitance (C) connected in parallel with a fast-activating slow-inactivating low-threshold K + current (I LT ), a high-threshold K + current (I HT ), a fast-inactivating TTX-sensitive Na + current (I N a ), a hyperpolarizationactivated cation current (I h ), a leakage current (I lk ), and an excitatory synaptic current (I E ).…”

mentioning

confidence: 99%

Influence of temperature on neuronal excitability in cochlear nucleus

2010

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The influence of temperature on neuronal excitability is studied by numerical simulations on the spiking threshold characteristics of bushy cells in cochlear nucleus periodically stimulated by synaptic currents. The results reveal that there is a cut-off frequency for the spiking of bushy cell in a specific temperature environment, corresponding to the existence of a critical temperature for the neuron to respond with real spikes to the synaptic stimulus of a given frequency, due to the finiteness of spike width. An optimal temperature range for neuronal spiking is also found for a specific stimulus frequency, and the temperature range span decreases with increasing stimulus frequency. These findings imply that there is a physiological temperature range which is beneficial for the information processing in auditory system. PACS numbers: 87. 19. Dd, 87. 10. +e, 87. 17. Aa, 87. 19. La Electric excitability has been attracting much attention for decades, for it is the basis of information processing and is essential to coding in neural systems. The excitability of a neuron originates from integrated effect of the kinetics [1], as well as the spatial distribution [2], of ion channels on the cellular membrane in proper environment, and determines the functional properties when responding to external stimulus, such as the frequency sensitivity [3,4] or selectivity [5], interaural time difference (ITD) sensitivity in auditory system [6], etc. Excitability and response properties of a neuron may vary in different environmental conditions of, say, temperature and pH values [7]. Recently, the influence of temperature on the excitability of rat suprachiasmatic nucleus neurons has been investigated experimentally, and the results reveal that there is a temperature-sensitive range for the neuronal activities; this may provide cues to the circadian synchronized rhythmicity [8,9]. Biophysically, temperature may influence the functioning of a neuron through the temperature dependence of various ion channel conductances and time constants of channel activation/inactivation variables [10]; hence changing temperature alters the basic properties of excitable neuron, such as the membrane potential, the input resistance, the shape and amplitude of action potentials, and the propagation of spikes [11,12,13,14]. Up to date, however, there has been little theoretical investigations of the influence of temperature on neuronal excitability in literature.Neuronal excitability can be described by the firing properties, like the spiking threshold of the neuron responding to periodic stimulus [5]. Characteristics of the spiking threshold of excitable neurons have been discussed in several studies [3,5,15,16,17], where the stimuli applied are mostly sinusoidal. More realistically, in fact, the stimulus to a post-synaptic neuron is often described by a current with alpha-function channel conductance [18], which is used in the present study. Comparable with Ref. [5], this work focuses on the effect of temperature on the spiking threshold of a...

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Structural arrangement of auditory brainstem nuclei in the bats Phyllostomus discolor and Carollia perspicillata

Pätz

Console-Meyer

Felmy

2022

J of Comparative Neurology

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The structure of the mammalian auditory brainstem is evolutionarily highly plastic, and distinct nuclei arrange in a species-dependent manner. Such anatomical variability is present in the superior olivary complex (SOC) and the nuclei of the lateral lemniscus (LL). Due to the structure-function relationship in the auditory brainstem, the identification of individual nuclei supports the understanding of sound processing. Here, we comparatively describe the nucleus arrangement and the expression of functional markers in the auditory brainstem of the two bat species Phyllostomus discolor and Carollia perspicillata. Using immunofluorescent labeling, we describe the arrangement and identity of the SOC and LL nuclei based on the expression of synaptic markers (vesicular glutamate transporter 1 and glycine transporter 2), calcium-binding proteins, as well as the voltage-gated ion channel subunits Kv1.1 and HCN1. The distribution of excitatory and inhibitory synaptic labeling appears similar between both species and matches with that of other mammals. The detection of calcium-binding proteins indicates species-dependent differences and deviations from other mammals. Kv1.1 and HCN1 show largely the same expression pattern in both species, which diverges from other mammals, indicating functional adaptations in the cellular physiology of bat neurons.

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Differential Expression of Three Distinct Potassium Currents in the Ventral Cochlear Nucleus

Cited by 104 publications

References 65 publications

Hyperpolarization-Activated Currents Regulate Excitability in Stellate Cells of the Mammalian Ventral Cochlear Nucleus

Hyperpolarization-Activated Currents Regulate Excitability in Stellate Cells of the Mammalian Ventral Cochlear Nucleus

Influence of temperature on neuronal excitability in cochlear nucleus

Structural arrangement of auditory brainstem nuclei in the bats Phyllostomus discolor and Carollia perspicillata

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