Differential expression of the soybean BiP gene family

Abstract

The soybean binding protein (BiP) gene family consists of at least four members designated soyBiPA, soyBiPB, soyBiPC and soyBiPD. We have performed immunoblotting of two-dimensional (2D) gels and RT-PCR assays with gene-specific primers to analyze the differential expression of this gene family in various soybean organs. The 2D gel profiles of the BiP forms from different organs were distinct and suggested that the BiP genes are under organ-specific regulation. In fact, while all four BiP transcripts were detected in leaves by gene-specific reverse transcriptase-polymerase chain reaction (RT-PCR) assays, different subsets were detected in the other organs. The soyBiPD was expressed in all organs, whereas the expression of the soyBiPB was restricted to leaves. The soyBiPA transcripts were detected in leaves, roots and seeds and soyBiPC RNA was confined to leaves, seeds and pods. Our data are consistent with organ-specific expression of the soybean BiP gene family.

Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins

“…1D) enhanced the solubility of total proteins compared with the other two methods. Cascardo and coworkers [18], Fig. 1.…”

“…This protocol was performed according to Cascardo and coworkers [18] with some modiWcations. The modiWcations included a diVerent amount of sample used, a diVerent incubation time, and a diVerent protein resolubilization buVer.…”

Evaluation of four protein extraction methods for proteomic analysis of mango peel

“…The protocol was performed according to Cascardo (Cascardo et al, 2001) with some modifications. The sample was added to ice-cold acetone containing 10% TCA (w/v), 0.07% b-mercaptoethanol (v/v) and homogenized on the ice.…”

Protein Separation in Functional Rice Grains Using Two-Dimensional Gel Electrophoresis

“…The proteins were extracted based on the method described of Cascardo et al (2001) with some modifications. Briefly, the white rice grains (100 mg) were frozen and ground into fine powder using liquid nitrogen.…”